Gene/Proteome Database (LMPD)

LMPD ID
LMP007305
Gene ID
856722
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
Ynd1p
Gene Symbol
YND1
Synonyms
APY1; YEJ5
Alternate Names
Ynd1p
Chromosome
V
EC Number
3.6.1.5

Proteins

Ynd1p
Refseq ID NP_010920
Protein GI 398364305
UniProt ID P40009
mRNA ID NM_001178896
Length 630
RefSeq Status PROVISIONAL
MLIENTNDRFGIVIDAGSSGSRIHVFKWQDTESLLHATNQDSQSILQSVPHIHQEKDWTFKLNPGLSSFEKKPQDAYKSHIKPLLDFAKNIIPESHWSSCPVFIQATAGMRLLPQDIQSSILDGLCQGLKHPAEFLVEDCSAQIQVIDGETEGLYGWLGLNYLYGHFNDYNPEVSDHFTFGFMDMGGASTQIAFAPHDSGEIARHRDDIATIFLRSVNGDLQKWDVFVSTWLGFGANQARRRYLAQLINTLPENTNDYENDDFSTRNLNDPCMPRGSSTDFEFKDTIFHIAGSGNYEQCTKSIYPLLLKNMPCDDEPCLFNGVHAPRIDFANDKFIGTSEYWYTANDVFKLGGEYNFDKFSKSLREFCNSNWTQILANSDKGVYNSIPENFLKDACFKGNWVLNILHEGFDMPRIDVDAENVNDRPLFQSVEKVEERELSWTLGRILLYASGSILAGNDDFMVGIAPSERRTKLTGKKFIPGKLLESDQLRKQSSSLSNKGFLMWFAIICCIFYLIFHRSHIIRRRFSGLYNITKDFKTGIRRRLKFLRRSDPFSRLEEGELGTDVDGFKDVYRMKSSSMFDLGKSSATMQREHEPQRTASQSANLAPSNLRPAFSMADFSKFKDSRLYD

Gene Information

Entrez Gene ID
856722
Gene Name
Ynd1p
Gene Symbol
YND1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0000139 IDA:SGD C Golgi membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:SGD C membrane
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0017110 IDA:SGD F nucleoside-diphosphatase activity
GO:0017111 IDA:SGD F nucleoside-triphosphatase activity
GO:0006486 IMP:SGD P protein glycosylation
GO:0006665 IEA:UniProtKB-KW P sphingolipid metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko00230 Purine metabolism
sce00230 Purine metabolism
sce00240 Pyrimidine metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-7177 UTP and CTP dephosphorylation II

Domain Information

InterPro Annotations

Accession Description
IPR000407 Nucleoside phosphatase GDA1/CD39

UniProt Annotations

Entry Information

Gene Name
Ynd1p
Protein Entry
YND1_YEAST
UniProt ID
P40009
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity A nucleoside 5'-triphosphate + 2 H(2)O = a nucleoside 5'-phosphate + 2 phosphate. {ECO:0000269|PubMed:10954728, ECO:0000269|PubMed:16227198}.
Cofactor Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250}; Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=A divalent cation Ca(2+), Mg(2+) or Mn(2+). {ECO:0000250};
Disruption Phenotype Cells are partially resistant to E4orf4 and can partially suppress the spindle checkpoint defect in CDC55 deletion mutant. Does not suppress the rapamycin-resistance of CDC55 deletion mutant. YND1 and CDC55 double mutant is fully resistant to E4orf4. Deletion mutant suppresses the synthetic lethality of triple mutants, where UPC2 and ECM22 are knocked out along with either HAP1, ERG6 or ERG28. {ECO:0000269|PubMed:16227198, ECO:0000269|PubMed:16702413}.
Enzyme Regulation Activity is inhibited both by interaction with VMA13 and by V-ATPase acidification of the lumen. The activity of VMA13 is not required for YND1 inhibition. {ECO:0000269|PubMed:10954728}.
Function Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Has equal high activity toward ADP/ATP, GDP/GTP, and UDP/UTP and approximately 50% less toward CDP/CTP and thiamine pyrophosphate. Has no activity toward GMP. Required for Golgi glycosylation and cell wall integrity. Together with CDC55, required for adenovirus E4orf4 (early region 4 open reading frame 4) induced toxicity, the apyrase activity is not required for this function. Plays a role in sphingolipid synthesis. {ECO:0000269|PubMed:10409709, ECO:0000269|PubMed:16227198, ECO:0000269|PubMed:16702413}.
Miscellaneous Present with 450 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Protein modification; protein glycosylation.
Similarity Belongs to the GDA1/CD39 NTPase family. {ECO:0000305}.
Subcellular Location Golgi apparatus {ECO:0000269|PubMed:10409709, ECO:0000269|PubMed:14562095}. Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Subunit Interacts with activator subunit VMA13 of vacuolar H(+)- ATPase. Interacts with CDC55; this interaction is disrupted by adenovirus E4orf4, which remains associated with both YND1 and CDC55. {ECO:0000269|PubMed:10954728, ECO:0000269|PubMed:16227198}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007305 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
398364305 RefSeq NP_010920 630 Ynd1p

Identical Sequences to LMP007305 proteins

Reference Database Accession Length Protein Name
GI:398364305 GenBank EIW11042.1 630 Ynd1p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:398364305 GenBank EWG86491.1 630 Ynd1p [Saccharomyces cerevisiae R008]
GI:398364305 GenBank EWG91393.1 630 Ynd1p [Saccharomyces cerevisiae P301]
GI:398364305 GenBank EWG96413.1 630 Ynd1p [Saccharomyces cerevisiae R103]
GI:398364305 GenBank EWH18763.1 630 Ynd1p [Saccharomyces cerevisiae P283]
GI:398364305 gnl McCuskerlabDuke 630 Ynd1p [Saccharomyces cerevisiae YJM993]

Related Sequences to LMP007305 proteins

Reference Database Accession Length Protein Name
GI:398364305 DBBJ GAA22828.1 630 K7_Ynd1p [Saccharomyces cerevisiae Kyokai no. 7]
GI:398364305 GenBank AAT92897.1 630 YER005W [Saccharomyces cerevisiae]
GI:398364305 GenBank EDN62971.1 630 NDPase/NTPase [Saccharomyces cerevisiae YJM789]
GI:398364305 GenBank EDZ72666.1 630 YER005Wp-like protein [Saccharomyces cerevisiae AWRI1631]
GI:398364305 GenBank EEU08693.1 630 Ynd1p [Saccharomyces cerevisiae JAY291]
GI:398364305 GenBank EGA83185.1 604 Ynd1p [Saccharomyces cerevisiae Lalvin QA23]