LIPID MAPS

LMPD ID

LMP007344

Gene ID

851634

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

serine C-palmitoyltransferase LCB2

Gene Symbol

LCB2

Synonyms

SCS1; TSC1

Chromosome

IV

EC Number

2.3.1.50

serine C-palmitoyltransferase LCB2
Refseq ID	NP_010347
Protein GI	6320267
UniProt ID	P40970
mRNA ID	NM_001180370
Length	561
MSTPANYTRVPLCEPEELPDDIQKENEYGTLDSPGHLYQVKSRHGKPLPEPVVDTPPYYISLLTYLNYLILIILGHVHDFLGMTFQKNKHLDLLEHDGLAPWFSNFESFYVRRIKMRIDDCFSRPTTGVPGRFIRCIDRISHNINEYFTYSGAVYPCMNLSSYNYLGFAQSKGQCTDAALESVDKYSIQSGGPRAQIGTTDLHIKAEKLVARFIGKEDALVFSMGYGTNANLFNAFLDKKCLVISDELNHTSIRTGVRLSGAAVRTFKHGDMVGLEKLIREQIVLGQPKTNRPWKKILICAEGLFSMEGTLCNLPKLVELKKKYKCYLFIDEAHSIGAMGPTGRGVCEIFGVDPKDVDILMGTFTKSFGAAGGYIAADQWIIDRLRLDLTTVSYSESMPAPVLAQTISSLQTISGEICPGQGTERLQRIAFNSRYLRLALQRLGFIVYGVADSPVIPLLLYCPSKMPAFSRMMLQRRIAVVVVAYPATPLIESRVRFCMSASLTKEDIDYLLRHVSEVGDKLNLKSNSGKSSYDGKRQRWDIEEVIRRTPEDCKDDKYFVN

Entrez Gene ID

851634

Gene Name

serine C-palmitoyltransferase LCB2

Gene Symbol

LCB2

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0017059	IMP:SGD	C	serine C-palmitoyltransferase complex
GO:0035339	IDA:UniProtKB	C	SPOTS complex
GO:0030170	IEA:InterPro	F	pyridoxal phosphate binding
GO:0004758	IMP:SGD	F	serine C-palmitoyltransferase activity
GO:0030148	IMP:SGD	P	sphingolipid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID	Description
sce01100	Metabolic pathways
ko00600	Sphingolipid metabolism
sce00600	Sphingolipid metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
SPHINGOLIPID-SYN-PWY	sphingolipid biosynthesis
SPHINGOLIPID-SYN-PWY	sphingolipid biosynthesis (yeast)

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618023	Metabolism
5618091	Metabolism of lipids and lipoproteins
5618508	Sphingolipid de novo biosynthesis
5618509	Sphingolipid metabolism

InterPro Annotations

Accession	Description
IPR004839	Aminotransferase, class I/classII
IPR001917	Aminotransferase, class-II, pyridoxal-phosphate binding site
IPR015424	Pyridoxal phosphate-dependent transferase
IPR015421	Pyridoxal phosphate-dependent transferase, major region, subdomain 1
IPR015422	Pyridoxal phosphate-dependent transferase, major region, subdomain 2

Entry Information

Gene Name

serine C-palmitoyltransferase LCB2

Protein Entry

LCB2_YEAST

UniProt ID

P40970

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D- sphinganine + CO(2). {ECO:0000269\|PubMed:10713067, ECO:0000269\|PubMed:11781309, ECO:0000269\|PubMed:15485854, ECO:0000269\|PubMed:8058731, ECO:0000269\|PubMed:8063782}.
Cofactor	Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
Function	Catalytic subunit of serine palmitoyltransferase (SPT), which catalyzes the committed step in the synthesis of sphingolipids, the condensation of serine with palmitoyl CoA to form the long chain base 3-ketosphinganine.
Interaction	P25045:LCB1; NbExp=9; IntAct=EBI-10067, EBI-10059;
Miscellaneous	Present with 54500 molecules/cell in log phase SD medium
Miscellaneous	Present with 54500 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Lipid metabolism; sphingolipid metabolism.
Similarity	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family
Similarity	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. {ECO:0000305}.
Subcellular Location	Cytoplasm. Endoplasmic reticulum. Membrane {ECO:0000305}; Multi-pass membrane protein .
Subcellular Location	Cytoplasm. Endoplasmic reticulum. Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
Subunit	LCB1 and LCB2 encode essential subunits of the enzyme and form a heterodimer. Component of the SPOTS complex, at least composed of LCB1/2 (LCB1 and/or LCB2), ORM1/2 (ORM1 and/or ORM2), SAC1 and TSC3. Interacts with LCB1 and TSC3. {ECO:0000269\|PubMed:10713067, ECO:0000269\|PubMed:11781309, ECO:0000269\|PubMed:15485854, ECO:0000269\|PubMed:20182505, ECO:0000269\|PubMed:8058731}.