Gene/Proteome Database (LMPD)

LMPD ID
LMP007362
Gene ID
850790
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
3-keto-steroid reductase
Gene Symbol
ERG27
Synonyms
-
Alternate Names
3-keto-steroid reductase
Chromosome
XII
EC Number
1.1.1.270

Proteins

3-keto-steroid reductase
Refseq ID NP_013201
Protein GI 6323129
UniProt ID Q12452
mRNA ID NM_001181987
Length 347
RefSeq Status PROVISIONAL
MNRKVAIVTGTNSNLGLNIVFRLIETEDTNVRLTIVVTSRTLPRVQEVINQIKDFYNKSGRVEDLEIDFDYLLVDFTNMVSVLNAYYDINKKYRAINYLFVNAAQGIFDGIDWIGAVKEVFTNPLEAVTNPTYKIQLVGVKSKDDMGLIFQANVFGPYYFISKILPQLTRGKAYIVWISSIMSDPKYLSLNDIELLKTNASYEGSKRLVDLLHLATYKDLKKLGINQYVVQPGIFTSHSFSEYLNFFTYFGMLCLFYLARLLGSPWHNIDGYKAANAPVYVTRLANPNFEKQDVKYGSATSRDGMPYIKTQEIDPTGMSDVFAYIQKKKLEWDEKLKDQIVETRTPI

Gene Information

Entrez Gene ID
850790
Gene Name
3-keto-steroid reductase
Gene Symbol
ERG27
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:SGD C endoplasmic reticulum
GO:0005789 IDA:SGD C endoplasmic reticulum membrane
GO:0005811 IDA:SGD C lipid particle
GO:0000253 IMP:SGD F 3-keto sterol reductase activity
GO:0006696 IMP:SGD P ergosterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
sce01100 Metabolic pathways
sce00100 Steroid biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID Description
ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5618346 Cholesterol biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
3-keto-steroid reductase
Protein Entry
ERG27_YEAST
UniProt ID
Q12452
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity A 3-beta-hydroxysteroid + NADP(+) = a 3- oxosteroid + NADPH.
Function Responsible for the reduction of the keto group on the C-3 of sterols. Also facilitates the association of ERG7 with lipid particles preventing its digestion in the endoplasmic reticulum and the lipid particles. {ECO:0000269|PubMed:10535978, ECO:0000269|PubMed:12842197}.
Interaction P53045:ERG25; NbExp=4; IntAct=EBI-38132, EBI-6506; P40030:ERG28; NbExp=6; IntAct=EBI-38132, EBI-22518; P38604:ERG7; NbExp=4; IntAct=EBI-38132, EBI-6572;
Pathway Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
Similarity Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Lipid droplet.
Subunit Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation- related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum. Interacts with ERG25 and ERG28. Also interacts with ERG7, but only in lipid particles. {ECO:0000269|PubMed:12119386, ECO:0000269|PubMed:12842197, ECO:0000269|PubMed:15995173}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007362 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6323129 RefSeq NP_013201 347 3-keto-steroid reductase

Identical Sequences to LMP007362 proteins

Reference Database Accession Length Protein Name
GI:6323129 GenBank ADS37606.1 347 Sequence 23 from patent US 7790428
GI:6323129 GenBank ADS37607.1 347 Sequence 24 from patent US 7790428
GI:6323129 GenBank EIW08719.1 347 Erg27p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6323129 GenBank EWG89886.1 347 Erg27p [Saccharomyces cerevisiae P301]
GI:6323129 GenBank EWG94709.1 347 Erg27p [Saccharomyces cerevisiae R103]
GI:6323129 Third Party Genbank DAA09417.1 347 TPA: 3-keto-steroid reductase [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007362 proteins

Reference Database Accession Length Protein Name
GI:6323129 GenBank EGA57803.1 347 Erg27p [Saccharomyces cerevisiae FostersB]
GI:6323129 GenBank EGA73948.1 347 Erg27p [Saccharomyces cerevisiae AWRI796]
GI:6323129 GenBank EGA85820.1 347 Erg27p [Saccharomyces cerevisiae VL3]
GI:6323129 GenBank EWG84369.1 347 Erg27p [Saccharomyces cerevisiae R008]
GI:6323129 GenBank EWH17265.1 347 Erg27p [Saccharomyces cerevisiae P283]
GI:6323129 gnl McCuskerlabDuke 347 Erg27p [Saccharomyces cerevisiae YJM993]