Gene/Proteome Database (LMPD)
Proteins
| Och1p | |
|---|---|
| Refseq ID | NP_011477 |
| Protein GI | 6321400 |
| UniProt ID | P31755 |
| mRNA ID | NM_001180903 |
| Length | 480 |
| RefSeq Status | PROVISIONAL |
| MSRKLSHLIATRKSKTIVVTVLLIYSLLTFHLSNKRLLSQFYPSKDDFKQTLLPTTSHSQDINLKKQITVNKKKNQLHNLRDQLSFAFPYDSQAPIPQRVWQTWKVGADDKNFPSSFRTYQKTWSGSYSPDYQYSLISDDSIIPFLENLYAPVPIVIQAFKLMPGNILKADFLRYLLLFARGGIYSDMDTMLLKPIDSWPSQNKSWLNNIIDLNKPIPYKNSKPSLLSSDEISHQPGLVIGIEADPDRDDWSEWYARRIQFCQWTIQAKPGHPILRELILNITATTLASVQNPGVPVSEMIDPRFEEDYNVNYRHKRRHDETYKHSELKNNKNVDGSDIMNWTGPGIFSDIIFEYMNNVLRYNSDILLINPNLNKNDEEGSESATTPAKDVDNDTLSKSTRKFYKKISESLQSSNSMPWEFFSFLKEPVIVDDVMVLPITSFSPDVGQMGAQSSDDKMAFVKHMFSGSWKEDADKNAGHK | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0000137 | IDA:SGD | C | Golgi cis cisterna |
| GO:0005783 | IEA:UniProtKB-KW | C | endoplasmic reticulum |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0000009 | IDA:SGD | F | alpha-1,6-mannosyltransferase activity |
| GO:0033164 | IEA:UniProtKB-EC | F | glycolipid 6-alpha-mannosyltransferase activity |
| GO:0016757 | ISS:SGD | F | transferase activity, transferring glycosyl groups |
| GO:0097502 | IDA:GOC | P | mannosylation |
| GO:0006487 | IMP:SGD | P | protein N-linked glycosylation |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| sce01100 | Metabolic pathways |
| M00074 | N-glycan biosynthesis, high-mannose type |
| sce_M00074 | N-glycan biosynthesis, high-mannose type |
| sce00513 | Various types of N-glycan biosynthesis |
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:17042779}; |
| Catalytic Activity | Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha- (1->6)-D-mannosyl-D-mannose linkage. {ECO:0000269|PubMed:1628616, ECO:0000269|PubMed:17042779}. |
| Cofactor | Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:17042779}; |
| Disruption Phenotype | Stops growing at the early stage of bud formation and rapidly loses viability at the non-permissive temperature. Exhibits a defect in the initiation of the mannose outer chain. Leads to accumulation of free forms of oligosaccharides (fOSs). {ECO:0000269|PubMed:10880465, ECO:0000269|PubMed:1523886, ECO:0000269|PubMed:1628616, ECO:0000269|PubMed:21622726, ECO:0000269|PubMed:7649302, ECO:0000269|PubMed:8253757}. |
| Domain | The conserved DXD motif is involved in enzyme activity. {ECO:0000303|PubMed:17042779}. |
| Function | Mannosyltransferase involved in outer chain elongation of asparagine-linked oligosaccharides of the type Man(9)GlcNAc(2). Adds the first alpha-1,6-mannose to the Man(8)GlNAc(2) and Man(9)GlcNAc(2), but not Man(5)GlcNAc(2), endoplasmic reticulum intermediates. Represents the first enzymatic event required for synthesis of outer chain mannose linkages on yeast secretory proteins. Has also the potential to transfer a second alpha-1,6- mannose to the Man(8)GlNAc(2) core oligosaccharide. {ECO:0000269|PubMed:1523886, ECO:0000269|PubMed:1628616, ECO:0000269|PubMed:17042779, ECO:0000269|PubMed:21979948, ECO:0000269|PubMed:7649302, ECO:0000269|PubMed:8253757}. |
| Induction | Expression is regulated by SKN7, SLN1, and CDC4. {ECO:0000269|PubMed:11036637, ECO:0000269|PubMed:11754484, ECO:0000269|PubMed:11854400}. |
| Miscellaneous | Present with 9490 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Ptm | Glycosylated. {ECO:0000269|PubMed:1628616}. |
| Similarity | Belongs to the glycosyltransferase 32 family. {ECO:0000305}. |
| Subcellular Location | Endoplasmic reticulum membrane {ECO:0000269|PubMed:14676282, ECO:0000269|PubMed:1628616}; Single- pass type II membrane protein {ECO:0000269|PubMed:8601597, ECO:0000303|PubMed:1628616}. Golgi apparatus membrane {ECO:0000269|PubMed:14676282, ECO:0000269|PubMed:15229219, ECO:0000269|PubMed:1628616}; Single-pass type II membrane protein {ECO:0000303|PubMed:1628616}. Note=Is recycled between the trans- Golgi network and a late compartment of the endoplasmic reticulum. {ECO:0000269|PubMed:14676282}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007376 (as displayed in Record Overview)
Identical Sequences to LMP007376 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6321400 | GenBank | EHN07093.1 | 480 | Och1p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7] |
| GI:6321400 | GenBank | EIW10314.1 | 480 | Och1p [Saccharomyces cerevisiae CEN.PK113-7D] |
| GI:6321400 | GenBank | EWG90962.1 | 480 | Och1p [Saccharomyces cerevisiae P301] |
| GI:6321400 | GenBank | EWG96063.1 | 480 | Och1p [Saccharomyces cerevisiae R103] |
| GI:6321400 | GenBank | EWH18241.1 | 480 | Och1p [Saccharomyces cerevisiae P283] |
| GI:6321400 | gnl | McCuskerlabDuke | 480 | Och1p [Saccharomyces cerevisiae YJM993] |
Related Sequences to LMP007376 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6321400 | DBBJ | GAA23350.1 | 480 | K7_Och1p [Saccharomyces cerevisiae Kyokai no. 7] |
| GI:6321400 | GenBank | EDO16164.1 | 460 | hypothetical protein Kpol_1026p11 [Vanderwaltozyma polyspora DSM 70294] |
| GI:6321400 | GenBank | EGA62317.1 | 318 | Och1p [Saccharomyces cerevisiae FostersO] |
| GI:6321400 | GenBank | EGA86783.1 | 318 | Och1p [Saccharomyces cerevisiae VL3] |
| GI:6321400 | GenBank | EHN02430.1 | 561 | Och1p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7] |
| GI:6321400 | GenBank | EJT43745.1 | 472 | OCH1-like protein [Saccharomyces kudriavzevii IFO 1802] |