Gene/Proteome Database (LMPD)

LMPD ID
LMP007404
Gene ID
851245
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
acetate--CoA ligase 1
Gene Symbol
ACS1
Synonyms
FUN44
Alternate Names
acetate--CoA ligase 1
Chromosome
I
EC Number
6.2.1.1

Proteins

acetate--CoA ligase 1
Refseq ID NP_009347
Protein GI 6319264
UniProt ID Q01574
mRNA ID NM_001178197
Length 713
RefSeq Status PROVISIONAL
MSPSAVQSSKLEEQSSEIDKLKAKMSQSAATAQQKKEHEYEHLTSVKIVPQRPISDRLQPAIATHYSPHLDGLQDYQRLHKESIEDPAKFFGSKATQFLNWSKPFDKVFIPDPKTGRPSFQNNAWFLNGQLNACYNCVDRHALKTPNKKAIIFEGDEPGQGYSITYKELLEEVCQVAQVLTYSMGVRKGDTVAVYMPMVPEAIITLLAISRIGAIHSVVFAGFSSNSLRDRINDGDSKVVITTDESNRGGKVIETKRIVDDALRETPGVRHVLVYRKTNNPSVAFHAPRDLDWATEKKKYKTYYPCTPVDSEDPLFLLYTSGSTGAPKGVQHSTAGYLLGALLTMRYTFDTHQEDVFFTAGDIGWITGHTYVVYGPLLYGCATLVFEGTPAYPNYSRYWDIIDEHKVTQFYVAPTALRLLKRAGDSYIENHSLKSLRCLGSVGEPIAAEVWEWYSEKIGKNEIPIVDTYWQTESGSHLVTPLAGGVTPMKPGSASFPFFGIDAVVLDPNTGEELNTSHAEGVLAVKAAWPSFARTIWKNHDRYLDTYLNPYPGYYFTGDGAAKDKDGYIWILGRVDDVVNVSGHRLSTAEIEAAIIEDPIVAECAVVGFNDDLTGQAVAAFVVLKNKSSWSTATDDELQDIKKHLVFTVRKDIGPFAAPKLIILVDDLPKTRSGKIMRRILRKILAGESDQLGDVSTLSNPGIVRHLIDSVKL

Gene Information

Entrez Gene ID
851245
Gene Name
acetate--CoA ligase 1
Gene Symbol
ACS1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:SGD C cytosol
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0005739 IEA:UniProtKB-KW C mitochondrion
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0003987 IDA:SGD F acetate-CoA ligase activity
GO:0016880 IDA:SGD F acid-ammonia (or amide) ligase activity
GO:0016208 IEA:InterPro F AMP binding
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0019654 IMP:SGD P acetate fermentation
GO:0006085 IDA:SGD P acetyl-CoA biosynthetic process
GO:0019427 IEA:InterPro P acetyl-CoA biosynthetic process from acetate
GO:0016573 IGI:SGD P histone acetylation

KEGG Pathway Links

KEGG Pathway ID Description
sce01200 Carbon metabolism
sce00010 Glycolysis / Gluconeogenesis
sce00680 Methane metabolism

Domain Information

InterPro Annotations

Accession Description
IPR011904 Acetate-CoA ligase
IPR020845 AMP-binding, conserved site
IPR025110 AMP-binding enzyme C-terminal domain
IPR000873 AMP-dependent synthetase/ligase

UniProt Annotations

Entry Information

Gene Name
acetate--CoA ligase 1
Protein Entry
ACS1_YEAST
UniProt ID
Q01574
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=0.32 mM for acetate {ECO:0000269|PubMed:8910545}; KM=1.4 mM for ATP {ECO:0000269|PubMed:8910545}; Vmax=1.1 umol/min/mg enzyme {ECO:0000269|PubMed:8910545};
Catalytic Activity ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.
Caution It is uncertain whether Met-1 or Met-25 is the initiator. {ECO:0000305}.
Function Catalyzes the production of acetyl-CoA. Provides the acetyl-CoA source for histone acetylation in the nucleus. "Aerobic" isozyme of acetyl-coenzyme A synthetase, which supports growth on nonfermentable carbon sources such as glycerol and ethanol. May be required for assimilation of ethanol and acetate. {ECO:0000269|PubMed:16857587, ECO:0000269|PubMed:8910545}.
Induction By acetate, acetaldehyde and ethanol. Subject to glucose catabolite repression. Inactivated and degraded after addition of glucose (at protein level). {ECO:0000269|PubMed:8910545, ECO:0000269|PubMed:9252575}.
Interaction Q02821:SRP1; NbExp=1; IntAct=EBI-2128, EBI-1797; P80235:YAT1; NbExp=1; IntAct=EBI-2128, EBI-3928; Q12110:YLR049C; NbExp=1; IntAct=EBI-2128, EBI-33635;
Miscellaneous Present with 2890 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Similarity Belongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}.
Subcellular Location Microsome {ECO:0000305}. Cytoplasm. Mitochondrion. Nucleus.

Identical and Related Proteins

Unique RefSeq proteins for LMP007404 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6319264 RefSeq NP_009347 713 acetate--CoA ligase 1

Identical Sequences to LMP007404 proteins

Reference Database Accession Length Protein Name
GI:6319264 GenBank AAC04979.1 713 Acs1p: acetyl CoA synthetase [Saccharomyces cerevisiae]
GI:6319264 GenBank ABZ27423.1 713 Sequence 1361 from patent US 7314974
GI:6319264 GenBank AGB63015.1 713 Sequence 30 from patent US 8288137
GI:6319264 SwissProt Q01574.2 713 RecName: Full=Acetyl-coenzyme A synthetase 1; AltName: Full=Acetate--CoA ligase 1; AltName: Full=Acyl-activating enzyme 1 [Saccharomyces cerevisiae S288c]
GI:6319264 Third Party Genbank DAA06934.1 713 TPA: acetate--CoA ligase 1 [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007404 proteins

Reference Database Accession Length Protein Name
GI:6319264 EMBL CAA47054.1 713 acetate--CoA ligase [Saccharomyces cerevisiae]
GI:6319264 EMBL CBN62157.1 713 unnamed protein product [Saccharomyces cerevisiae]
GI:6319264 EMBL CBN59940.1 713 unnamed protein product [Saccharomyces cerevisiae]
GI:6319264 GenBank AAU09675.1 713 YAL054C [Saccharomyces cerevisiae]
GI:6319264 GenBank EDN59709.1 713 acetyl CoA synthetase [Saccharomyces cerevisiae YJM789]
GI:6319264 GenBank AHH77100.1 713 Sequence 17 from patent US 8628922