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Catalytic Activity
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Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
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Function
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Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
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Interaction
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Q99380:OST4; NbExp=2; IntAct=EBI-12680, EBI-12689; P39007:STT3; NbExp=2; IntAct=EBI-12680, EBI-18447;
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Miscellaneous
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Present with 4030 molecules/cell in log phase SD medium
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Miscellaneous
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Present with 4030 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
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Pathway
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Protein modification; protein glycosylation.
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Similarity
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Belongs to the OST3/OST6 family
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Similarity
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Belongs to the OST3/OST6 family. {ECO:0000305}.
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Similarity
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Contains 1 thioredoxin domain
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Similarity
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Contains 1 thioredoxin domain. {ECO:0000305}.
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Subcellular Location
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Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein .
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Subcellular Location
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Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
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Subunit
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Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST3, OST4 and STT3 probably form a subcomplex. May interact directly with OST1, OST2, OST4 and STT3. {ECO:0000269|PubMed:8175708, ECO:0000269|PubMed:9405463}.
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