LIPID MAPS

LMPD ID

LMP007407

Gene ID

854371

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

putative lipoate synthase

Gene Symbol

LIP5

Synonyms

-

Chromosome

XV

EC Number

2.8.1.8

putative lipoate synthase
Refseq ID	NP_014839
Protein GI	6324770
UniProt ID	P32875
mRNA ID	NM_001183615
Length	414
MYRRSVGVLFVGRNTRWISSTIRCGTSATRPIRSNALNTDSDNASVRVPVGNSTEVENATSQLTGTSGKRRKGNRKRITEFKDALNLGPSFADFVSGKASKMILDPLEKARQNTEEAKKLPRWLKVPIPKGTNYHKLKGDVKELGLSTVCEEARCPNIGECWGGKDKSKATATIMLLGDTCTRGCRFCSVKTNRTPSKPDPMEPENTAEAIKRWGLGYVVLTTVDRDDLVDGGANHLAETVRKIKQKAPNTLVETLSGDFRGDLKMVDIMAQCGLDVYAHNLETVESLTPHVRDRRATYRQSLSVLERAKATVPSLITKTSIMLGLGETDEQITQTLKDLRNIQCDVVTFGQYMRPTKRHMKVVEYVKPEKFDYWKERALEMGFLYCASGPLVRSSYKAGEAFIENVLKKRNMK

Entrez Gene ID

854371

Gene Name

putative lipoate synthase

Gene Symbol

LIP5

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005739	IEA:UniProtKB-HAMAP	C	mitochondrion
GO:0051539	IEA:UniProtKB-HAMAP	F	4 iron, 4 sulfur cluster binding
GO:0016992	ISA:SGD	F	lipoate synthase activity
GO:0046872	IEA:UniProtKB-KW	F	metal ion binding
GO:0009249	IMP:SGD	P	protein lipoylation

KEGG Pathway Links

KEGG Pathway ID	Description
ko00785	Lipoic acid metabolism
sce00785	Lipoic acid metabolism
sce01100	Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY-7381	lipoate biosynthesis and incorporation (glycine cleavage system)
PWY-7381	lipoate biosynthesis and incorporation (glycine cleavage system, yeast)
PWY0-501	lipoate biosynthesis and incorporation I
PWY0-1275	lipoate biosynthesis and incorporation II
PWY-7382	lipoate biosynthesis and incorporation (pyruvate dehydrogenase and oxoglutarate dehydrogenase)
PWY-7382	lipoate biosynthesis and incorporation (pyruvate dehydrogenase and oxoglutarate dehydrogenase, yeast)
PWY-7386	superpathway of lipoate biosynthesis and incorporation (PDH, KGDH, GCV)
PWY-7386	superpathway of lipoate biosynthesis and incorporation (PDH, KGDH, GCV, yeast)

InterPro Annotations

Accession	Description
IPR013785	Aldolase-type TIM barrel
IPR006638	Elongator protein 3/MiaB/NifB
IPR003698	Lipoyl synthase
IPR007197	Radical SAM

Entry Information

Gene Name

putative lipoate synthase

Protein Entry

LIPA_YEAST

UniProt ID

P32875

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	Protein N(6)-(octanoyl)lysine + 2 sulfur- (sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)- (lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'- deoxyadenosine.
Cofactor	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000255\|HAMAP-Rule:MF_03123}; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L- methionine. {ECO:0000255\|HAMAP-Rule:MF_03123};
Cofactor	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence= ; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L- methionine. ;
Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives
Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. {ECO:0000305\|PubMed:19570983, ECO:0000305\|PubMed:8349643}.
Miscellaneous	Present with 1630 molecules/cell in log phase SD medium
Miscellaneous	Present with 1630 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2. {ECO:0000255\|HAMAP-Rule:MF_03123}.
Similarity	Belongs to the radical SAM superfamily. Lipoyl synthase family
Similarity	Belongs to the radical SAM superfamily. Lipoyl synthase family. {ECO:0000255\|HAMAP-Rule:MF_03123}.
Subcellular Location	Mitochondrion {ECO:0000255\|HAMAP- Rule:MF_03123, ECO:0000269\|PubMed:14562095, ECO:0000269\|PubMed:14576278}.