LIPID MAPS

LMPD ID

LMP007413

Gene ID

853707

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

Mcr1p

Gene Symbol

MCR1

Synonyms

-

Chromosome

XI

EC Number

1.6.2.2

Mcr1p
Refseq ID	NP_012772
Protein GI	6322699
UniProt ID	P36060
mRNA ID	NM_001179716
Length	302
MFSRLSRSHSKALPIALGTVAIAAATAFYFANRNQHSFVFNESNKVFKGDDKWIDLPISKIEEESHDTRRFTFKLPTEDSEMGLVLASALFAKFVTPKGSNVVRPYTPVSDLSQKGHFQLVVKHYEGGKMTSHLFGLKPNDTVSFKGPIMKWKWQPNQFKSITLLGAGTGINPLYQLAHHIVENPNDKTKVNLLYGNKTPQDILLRKELDALKEKYPDKFNVTYFVDDKQDDQDFDGEISFISKDFIQEHVPGPKESTHLFVCGPPPFMNAYSGEKKSPKDQGELIGILNNLGYSKDQVFKF

Entrez Gene ID

853707

Gene Name

Mcr1p

Gene Symbol

MCR1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0031307	IDA:SGD	C	integral component of mitochondrial outer membrane
GO:0005758	IDA:SGD	C	mitochondrial intermembrane space
GO:0005741	IDA:SGD	C	mitochondrial outer membrane
GO:0004128	IDA:SGD	F	cytochrome-b5 reductase activity, acting on NAD(P)H
GO:0034599	IMP:SGD	P	cellular response to oxidative stress
GO:0006696	IDA:SGD	P	ergosterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID	Description
ko00520	Amino sugar and nucleotide sugar metabolism
sce00520	Amino sugar and nucleotide sugar metabolism
sce01110	Biosynthesis of secondary metabolites

InterPro Annotations

Accession	Description
IPR017927	Ferredoxin reductase-type FAD-binding domain
IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase
IPR001834	NADH:cytochrome b5 reductase (CBR)
IPR001433	Oxidoreductase FAD/NAD(P)-binding
IPR008333	Oxidoreductase, FAD-binding domain
IPR017938	Riboflavin synthase-like beta-barrel

Entry Information

Gene Name

Mcr1p

Protein Entry

MCR1_YEAST

UniProt ID

P36060

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5.
Cofactor	Name=FAD; Xref=ChEBI:CHEBI:57692;
Function	The outer membrane form may mediate the reduction of outer membrane cytochrome b5, and the soluble inter-membrane space form may transfer electrons from external NADH to cytochrome c, thereby mediating an antimycin-insensitive, energy-coupled oxidation of external NADH by yeast mitochondria. Involved in the reduction of D-erythroascorbyl free radicals
Function	The outer membrane form may mediate the reduction of outer membrane cytochrome b5, and the soluble inter-membrane space form may transfer electrons from external NADH to cytochrome c, thereby mediating an antimycin-insensitive, energy-coupled oxidation of external NADH by yeast mitochondria. Involved in the reduction of D-erythroascorbyl free radicals. {ECO:0000269\|PubMed:11420140}.
Induction	By osmotic stress
Induction	By osmotic stress. {ECO:0000269\|PubMed:15590813}.
Interaction	P39940:RSP5; NbExp=2; IntAct=EBI-10565, EBI-16219;
Mass Spectrometry	Mass=34137; Method=Unknown; Range=1-302; Evidence= ;
Mass Spectrometry	Mass=34137; Method=Unknown; Range=1-302; Evidence={ECO:0000269\|PubMed:16689936};
Miscellaneous	Present with 1920 molecules/cell in log phase SD medium
Miscellaneous	Present with 1920 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Ptm	There are two isoforms of NADH-cytochrome b5 reductase, a 34 kDa form (p34) and a 32 kDa form (p32). The p34 form becomes firmly anchored to the outer mitochondrial membrane after an incomplete translocation arrest. The p32 form is formed after translocation of the p34 precursor to the inner mitochondrial membrane, where it is processed by mitochondrial inner membrane peptidase (IMP) complex and released to the intermembrane space.
Similarity	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family
Similarity	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. {ECO:0000305}.
Similarity	Contains 1 FAD-binding FR-type domain
Similarity	Contains 1 FAD-binding FR-type domain. {ECO:0000255\|PROSITE-ProRule:PRU00716}.
Subcellular Location	NADH-cytochrome b5 reductase p32 form: Mitochondrion intermembrane space.
Subcellular Location	NADH-cytochrome b5 reductase p34 form: Mitochondrion outer membrane; Single-pass membrane protein.