Gene/Proteome Database (LMPD)

LMPD ID
LMP007433
Gene ID
853061
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
phosphatidylethanolamine N-methyltransferase
Gene Symbol
CHO2
Synonyms
PEM1
Chromosome
VII
EC Number
2.1.1.17

Proteins

phosphatidylethanolamine N-methyltransferase
Refseq ID NP_011673
Protein GI 6321596
UniProt ID P05374
mRNA ID NM_001181286
Length 869
MSSCKTTLSEMVGSVTKDRGTINVEARTRSSNVTFKPPVTHDMVRSLFDPTLKKSLLEKCIALAIISNFFICYWVFQRFGLQFTKYFFLVQYLFWRIAYNLGIGLVLHYQSHYETLTNCAKTHAIFSKIPQNKDANSNFSTNSNSFSEKFWNFIRKFCQYEIRSKMPKEYDLFAYPEEINVWLIFRQFVDLILMQDFVTYIIYVYLSIPYSWVQIFNWRSLLGVILILFNIWVKLDAHRVVKDYAWYWGDFFFLEESELIFDGVFNISPHPMYSIGYLGYYGLSLICNDYKVLLVSVFGHYSQFLFLKYVENPHIERTYGDGTDSDSQMNSRIDDLISKENYDYSRPLINMGLSFNNFNKLRFTDYFTIGTVAALMLGTIMNARFINLNYLFITVFVTKLVSWLFISTILYKQSQSKWFTRLFLENGYTQVYSYEQWQFIYNYYLVLTYTLMIIHTGLQIWSNFSNINNSQLIFGLILVALQTWCDKETRLAISDFGWFYGDFFLSNYISTRKLTSQGIYRYLNHPEAVLGVVGVWGTVLMTNFAVTNIILAVLWTLTNFILVKFIETPHVNKIYGKTKRVSGVGKTLLGLKPLRQVSDIVNRIENIIIKSLVDESKNSNGGAELLPKNYQDNKEWNILIQEAMDSVATRLSPYCELKIENEQVETNFVLPTPVTLNWKMPIELYNGDDWIGLYKVIDTRADREKTRVGSGGHWSATSKDSYMNHGLRHKESVTEIKATEKYVQGKVTFDTSLLYFENGIYEFRYHSGNSHKVLLISTPFEISLPVLNTTTPELFEKDLTEFLTKVNVLKDGKFRPLGNKFFGMDSLKQLIKNSIGVELSSEYMRRVNGDAHVISHRAWDIKQTLDSLA

Gene Information

Entrez Gene ID
853061
Gene Name
phosphatidylethanolamine N-methyltransferase
Gene Symbol
CHO2
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0004608 IDA:SGD F phosphatidylethanolamine N-methyltransferase activity
GO:0006656 IDA:SGD P phosphatidylcholine biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ko00564 Glycerophospholipid metabolism
sce00564 Glycerophospholipid metabolism
sce01100 Metabolic pathways
M00091 Phosphatidylcholine (PC) biosynthesis, PE => PC
sce_M00091 Phosphatidylcholine (PC) biosynthesis, PE => PC

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6825 phosphatidylcholine biosynthesis V
PWY-6825 phosphatidylcholine biosynthesis V

Domain Information

InterPro Annotations

Accession Description
IPR016219 Phosphatid-EA_MeTrfase_fun
IPR007318 Phospholipid methyltransferase

UniProt Annotations

Entry Information

Gene Name
phosphatidylethanolamine N-methyltransferase
Protein Entry
CHO2_YEAST
UniProt ID
P05374
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=60 uM for S-adenosyl-L-methionine {ECO:0000269|PubMed:2684666}; pH dependence: Optimum pH is 9.9. {ECO:0000269|PubMed:2684666};
Biophysicochemical Properties Kinetic parameters: KM=60 uM for S-adenosyl-L-methionine ; pH dependence: Optimum pH is 9.9. ;
Catalytic Activity S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.
Function Catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis. Preferentially converts di-C16:1 substrates. {ECO:0000269|PubMed:15258140, ECO:0000269|PubMed:2684666, ECO:0000269|PubMed:2850468, ECO:0000269|PubMed:3025587, ECO:0000269|PubMed:3066687, ECO:0000269|PubMed:6759124, ECO:0000269|PubMed:6988218}.
Induction Repressed by myo-inositol and choline
Induction Repressed by myo-inositol and choline. {ECO:0000269|PubMed:1650774, ECO:0000269|PubMed:1954254}.
Interaction P25376:AGP1; NbExp=1; IntAct=EBI-2049142, EBI-2357; P08456:CHO1; NbExp=1; IntAct=EBI-2049142, EBI-14055; P39540:ELO1; NbExp=1; IntAct=EBI-2049142, EBI-2049096; P10614:ERG11; NbExp=1; IntAct=EBI-2049142, EBI-5127; P41543:OST1; NbExp=1; IntAct=EBI-2049142, EBI-12651; P40975:PMP2; NbExp=1; IntAct=EBI-2049142, EBI-2043041; Q04947:RTN1; NbExp=1; IntAct=EBI-2049142, EBI-38020; P32368:SAC1; NbExp=1; IntAct=EBI-2049142, EBI-16210; P39986:SPF1; NbExp=1; IntAct=EBI-2049142, EBI-3128; P38800:YHR080C; NbExp=1; IntAct=EBI-2049142, EBI-24597;
Miscellaneous Present with 1810 molecules/cell in log phase SD medium
Miscellaneous Present with 1810 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Phospholipid metabolism; phosphatidylcholine biosynthesis.
Similarity Belongs to the class VI-like SAM-binding methyltransferase superfamily. CHO2 family
Similarity Belongs to the class VI-like SAM-binding methyltransferase superfamily. CHO2 family. {ECO:0000305}.
Subcellular Location Membrane; Multi-pass membrane protein.

Identical and Related Proteins

Unique RefSeq proteins for LMP007433 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6321596 RefSeq NP_011673 869 phosphatidylethanolamine N-methyltransferase

Identical Sequences to LMP007433 proteins

Reference Database Accession Length Protein Name

Related Sequences to LMP007433 proteins

Reference Database Accession Length Protein Name