Gene/Proteome Database (LMPD)

LMPD ID
LMP007440
Gene ID
851462
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
Pmt1p
Gene Symbol
PMT1
Synonyms
-
Alternate Names
Pmt1p
Chromosome
IV
EC Number
2.4.1.109

Proteins

Pmt1p
Refseq ID NP_010188
Protein GI 6320107
UniProt ID P33775
mRNA ID NM_001180154
Length 817
RefSeq Status PROVISIONAL
MSEEKTYKRVEQDDPVPELDIKQGPVRPFIVTDPSAELASLRTMVTLKEKLLVACLAVFTAVIRLHGLAWPDSVVFDEVHFGGFASQYIRGTYFMDVHPPLAKMLYAGVASLGGFQGDFDFENIGDSFPSTTPYVLMRFFSASLGALTVILMYMTLRYSGVRMWVALMSAICFAVENSYVTISRYILLDAPLMFFIAAAVYSFKKYEMYPANSLNAYKSLLATGIALGMASSSKWVGLFTVTWVGLLCIWRLWFMIGDLTKSSKSIFKVAFAKLAFLLGVPFALYLVFFYIHFQSLTLDGDGASFFSPEFRSTLKNNKIPQNVVADVGIGSIISLRHLSTMGGYLHSHSHNYPAGSEQQQSTLYPHMDANNDWLLELYNAPGESLTTFQNLTDGTKVRLFHTVTRCRLHSHDHKPPVSESSDWQKEVSCYGYSGFDGDANDDWVVEIDKKNSAPGVAQERVIALDTKFRLRHAMTGCYLFSHEVKLPAWGFEQQEVTCASSGRHDLTLWYVENNSNPLLPEDTKRISYKPASFISKFIESHKKMWHINKNLVEPHVYESQPTSWPFLLRGISYWGENNRNVYLLGNAIVWWAVTAFIGIFGLIVITELFSWQLGKPILKDSKVVNFHVQVIHYLLGFAVHYAPSFLMQRQMFLHHYLPAYYFGILALGHALDIIVSYVFRSKRQMGYAVVITFLAASVYFFKSFSPIIYGTPWTQELCQKSQWLSGWDYNCNTYFSSLEEYKNQTLTKRESQPAATSTVEEITIEGDGPSYEDLMNEDGKKIFKDTEGNELDPEVVKKMLEEEGANILKVEKRAVLE

Gene Information

Entrez Gene ID
851462
Gene Name
Pmt1p
Gene Symbol
PMT1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0097582 IDA:SGD C dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt2p dimer complex
GO:0097583 IDA:SGD C dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt3p dimer complex
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0004169 IDA:SGD F dolichyl-phosphate-mannose-protein mannosyltransferase activity
GO:0071712 IMP:SGD P ER-associated misfolded protein catabolic process
GO:0035269 IDA:SGD P protein O-linked mannosylation
GO:0032527 IGI:SGD P protein exit from endoplasmic reticulum
GO:1900101 IMP:SGD P regulation of endoplasmic reticulum unfolded protein response

KEGG Pathway Links

KEGG Pathway ID Description
ko00514 Other types of O-glycan biosynthesis
sce00514 Other types of O-glycan biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR027004 Dolichyl-phosphate-mannose-protein mannosyltransferase 1/5
IPR003342 Glycosyl transferase, family 39
IPR027005 Glycosyltransferase 39 like
IPR016093 MIR_motif

UniProt Annotations

Entry Information

Gene Name
Pmt1p
Protein Entry
PMT1_YEAST
UniProt ID
P33775
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity Dolichyl D-mannosyl phosphate + protein = dolichyl phosphate + O-D-mannosylprotein. {ECO:0000269|PubMed:10764776, ECO:0000269|PubMed:8367478}.
Disruption Phenotype Affects glycosylation of chitinase and increases sensitivity to calcofluor white and caffeine. {ECO:0000269|PubMed:8918452}.
Domain The large luminal loop 5 is essential for mannosyltransferase activity but not for PMT1-PMT2 complex formation. {ECO:0000269|PubMed:10764776}.
Function Protein O-mannosyltransferase involved in O- glycosylation which is essential for cell wall rigidity. Forms a heterodimeric complex with PMT2 and more rarely with PMT3 to transfer mannose from Dol-P-mannose to Ser or Thr residues on proteins. The PMT1-PMT2 complex participates to oxidative protein folding, ER-associated protein degradation (ERAD), as well as ER export. Required for incorporation of proteins in the cell wall. {ECO:0000269|PubMed:10764776, ECO:0000269|PubMed:18182384, ECO:0000269|PubMed:8367478, ECO:0000269|PubMed:8543034, ECO:0000269|PubMed:9466258}.
Interaction P31382:PMT2; NbExp=4; IntAct=EBI-13567, EBI-13573; P47190:PMT3; NbExp=3; IntAct=EBI-13567, EBI-13579;
Miscellaneous Present with 41500 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Protein modification; protein glycosylation. {ECO:0000305}.
Similarity Belongs to the glycosyltransferase 39 family. {ECO:0000305}.
Similarity Contains 3 MIR domains. {ECO:0000255|PROSITE- ProRule:PRU00131}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000303|PubMed:10085156}; Multi-pass membrane protein {ECO:0000269|PubMed:10085156}.
Subunit PMT1 and PMT2 form a functional heterodimer. The complex interacts with endoplasmic reticulum proteins EMP24, ERV25, ERP1, ERP2, CDC48, HRD1, USA1, YOS9, ERO1, PDI1, UBR1, Cue4, DFM1 and TED1. Forms also a minor complex with PMT3. {ECO:0000269|PubMed:12551906, ECO:0000269|PubMed:21147851, ECO:0000269|PubMed:8543034}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007440 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6320107 RefSeq NP_010188 817 Pmt1p

Identical Sequences to LMP007440 proteins

Reference Database Accession Length Protein Name
GI:6320107 EMBL CAA64917.1 817 PMT1 [Saccharomyces cerevisiae]
GI:6320107 EMBL CAA98663.1 817 PMT1 [Saccharomyces cerevisiae]
GI:6320107 EMBL CAA02295.1 817 PMT1 GENE [Saccharomyces cerevisiae]
GI:6320107 pat US 817 Sequence 2 from patent US 5714377
GI:6320107 SwissProt P33775.1 817 RecName: Full=Dolichyl-phosphate-mannose--protein mannosyltransferase 1 [Saccharomyces cerevisiae S288c]
GI:6320107 Third Party Genbank DAA11764.1 817 TPA: Pmt1p [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007440 proteins

Reference Database Accession Length Protein Name
GI:6320107 DBBJ GAA22154.1 817 K7_Pmt1p [Saccharomyces cerevisiae Kyokai no. 7]
GI:6320107 GenBank EIW11112.1 817 Pmt1p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6320107 GenBank EWG86986.1 817 Pmt1p [Saccharomyces cerevisiae R008]
GI:6320107 GenBank EWG91698.1 817 Pmt1p [Saccharomyces cerevisiae P301]
GI:6320107 GenBank EWG96910.1 817 Pmt1p [Saccharomyces cerevisiae R103]
GI:6320107 gnl McCuskerlabDuke 817 Pmt1p [Saccharomyces cerevisiae YJM993]