Gene/Proteome Database (LMPD)

LMPD ID
LMP007444
Gene ID
853613
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
branched-chain-amino-acid transaminase BAT2
Gene Symbol
BAT2
Synonyms
ECA40; TWT2
Alternate Names
branched-chain-amino-acid transaminase BAT2
Chromosome
X
EC Number
2.6.1.42

Proteins

branched-chain-amino-acid transaminase BAT2
Refseq ID NP_012682
Protein GI 6322608
UniProt ID P47176
mRNA ID NM_001181806
Length 376
RefSeq Status PROVISIONAL
MTLAPLDASKVKITTTQHASKPKPNSELVFGKSFTDHMLTAEWTAEKGWGTPEIKPYQNLSLDPSAVVFHYAFELFEGMKAYRTVDNKITMFRPDMNMKRMNKSAQRICLPTFDPEELITLIGKLIQQDKCLVPEGKGYSLYIRPTLIGTTAGLGVSTPDRALLYVICCPVGPYYKTGFKAVRLEATDYATRAWPGGCGDKKLGANYAPCVLPQLQAASRGYQQNLWLFGPNNNITEVGTMNAFFVFKDSKTGKKELVTAPLDGTILEGVTRDSILNLAKERLEPSEWTISERYFTIGEVTERSKNGELLEAFGSGTAAIVSPIKEIGWKGEQINIPLLPGEQTGPLAKEVAQWINGIQYGETEHGNWSRVVTDLN

Gene Information

Entrez Gene ID
853613
Gene Name
branched-chain-amino-acid transaminase BAT2
Gene Symbol
BAT2
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:SGD C cytoplasm
GO:0052656 IEA:UniProtKB-EC F L-isoleucine transaminase activity
GO:0052654 IEA:UniProtKB-EC F L-leucine transaminase activity
GO:0052655 IEA:UniProtKB-EC F L-valine transaminase activity
GO:0004084 IDA:SGD F branched-chain-amino-acid transaminase activity
GO:0019509 IEA:UniProtKB-UniPathway P L-methionine biosynthetic process from methylthioadenosine
GO:0009082 IDA:SGD P branched-chain amino acid biosynthetic process
GO:0009083 IMP:SGD P branched-chain amino acid catabolic process
GO:0009097 IEA:UniProtKB-UniPathway P isoleucine biosynthetic process
GO:0009098 IEA:UniProtKB-UniPathway P leucine biosynthetic process
GO:0009099 IEA:UniProtKB-UniPathway P valine biosynthetic process

Domain Information

InterPro Annotations

Accession Description
IPR001544 Aminotransferase, class IV
IPR018300 Aminotransferase, class IV, conserved site
IPR005786 Branched-chain amino acid aminotransferase II

UniProt Annotations

Entry Information

Gene Name
branched-chain-amino-acid transaminase BAT2
Protein Entry
BCA2_YEAST
UniProt ID
P47176
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity L-isoleucine + 2-oxoglutarate = (S)-3-methyl- 2-oxopentanoate + L-glutamate.
Catalytic Activity L-leucine + 2-oxoglutarate = 4-methyl-2- oxopentanoate + L-glutamate.
Catalytic Activity L-valine + 2-oxoglutarate = 3-methyl-2- oxobutanoate + L-glutamate.
Cofactor Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
Developmental Stage Highly expressed during stationary phase, down-regulated during logarithmic phase of growth.
Function Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Catalyzes the formation of methionine from 2-keto-4- methylthiobutyrate (KMTB) in the methionine salvage pathway primarily using branched chain amino acids (leucine, isoleucine, and valine) as well as lysine and proline as the amino donors. Involved in cell cycle regulation. {ECO:0000269|PubMed:18625006, ECO:0000269|PubMed:21267457}.
Induction Down-regulated in the presence of repressive nitrogen sources (glutamine) and derepressed in secondary non-repressive nitrogen sources such as GABA. Highly expressed on cultures with isoleucine, leucine and valine as sole nitrogen source (catabolic conditions). {ECO:0000269|PubMed:21267457}.
Interaction P22696:ESS1; NbExp=1; IntAct=EBI-3462, EBI-6679;
Miscellaneous Present with 25900 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Amino-acid biosynthesis; L-isoleucine biosynthesis; L- isoleucine from 2-oxobutanoate: step 4/4.
Pathway Amino-acid biosynthesis; L-leucine biosynthesis; L- leucine from 3-methyl-2-oxobutanoate: step 4/4.
Pathway Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 6/6.
Pathway Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.
Sequence Caution Sequence=CAA60376.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAA60376.1; Type=Frameshift; Positions=1, 170, 199, 207, 210, 214; Evidence={ECO:0000305};
Similarity Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. {ECO:0000305}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:21267457}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007444 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6322608 RefSeq NP_012682 376 branched-chain-amino-acid transaminase BAT2

Identical Sequences to LMP007444 proteins

Reference Database Accession Length Protein Name
GI:6322608 GenBank AEN29028.1 376 Sequence 238 from patent US 7993889
GI:6322608 GenBank AFL35599.1 376 Sequence 238 from patent US 8178328
GI:6322608 GenBank EIW09674.1 376 Bat2p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6322608 GenBank AFT61855.1 376 Sequence 238 from patent US 8273558
GI:6322608 GenBank AFX06641.1 376 Sequence 238 from patent US 8283144
GI:6322608 GenBank AGV92098.1 376 Sequence 12 from patent US 8530226

Related Sequences to LMP007444 proteins

Reference Database Accession Length Protein Name
GI:6322608 GenBank EDN63464.1 376 branched-chain amino acid transaminase [Saccharomyces cerevisiae YJM789]
GI:6322608 GenBank EDZ71108.1 376 YJR148Wp-like protein [Saccharomyces cerevisiae AWRI1631]
GI:6322608 GenBank EGA74234.1 376 Bat2p [Saccharomyces cerevisiae AWRI796]
GI:6322608 GenBank EWG88064.1 376 Bat2p [Saccharomyces cerevisiae P301]
GI:6322608 GenBank EWG95022.1 376 Bat2p [Saccharomyces cerevisiae R103]
GI:6322608 GenBank EWH17684.1 376 Bat2p [Saccharomyces cerevisiae P283]