LIPID MAPS

LMPD ID

LMP007464

Gene ID

853902

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

phospholipase D

Gene Symbol

SPO14

Synonyms

PLD1

Chromosome

XI

EC Number

3.1.4.4

phospholipase D
Refseq ID	NP_012956
Protein GI	398365057
UniProt ID	P36126
mRNA ID	NM_001179821
Length	1683
MSNVSTASGTHFAPPQADRSVTEEVDRVNSRPDELENQEVLRQLPENGNLTSSLQREKRRTPNGKEAERKHALPKSFVDRNLSDVSPNHSLDHIMHSNEHDPRRGSDEENMHRLYNNLHSSNNNVHSKRNSKREEERAPQRRSSSVAYTQQQFNGWKKEFGHAFKKISAIGRLKSSVNSPTPAGSGHRHNQHQHQQVNEEDLYTQRLASDLLDSLLAGCPASLFASTQFLRDEHGKRRAPLLLAKLDVRVSPLKNDNNILDITNSNHNHRGNNNNNTGENSDRRPSIPRSSSIISISSNVAEFMYSRNENSLFRIHLEYGIDEDRLKWSIIRSYKDIKSLHHKLKIVAFQQLTISKLYSDNNRYHSLQLPHFPHYKEMVKERNVMEKKAENKPSSAASAPHTSENNNNDNGSNITSLETLSSSEISEFNIDNVKMKHLQDLIDEPDDFSQPIHLRLERYLRLLNIALCLRPHANRLFEFYELSPLGNLLSRESGFQGKQGYLVIRSTAKAQGWRVSHFGKHAFKDMIDRHTTKWFLVRNSYLTYVSDLSSTTPLDVFLIDWKFKVRFSGNKNNILDNENEINWIIHDPNLEINDELEEFGIENDANNILDKNGKSKTHQKKSNISSKLLLLTLENSERKLKIICKSESSLKQWMSSIIKMSTSTPWSKPNRFGSFAPVRTNSFCKFLVDGRDYFWSLSEALLMAKDVIYIHDWWLSPELYLRRPVKGNQGFRIDRMLKSCAEKGIKIFIVIYRNVGNIVGTDSLWTKHSMLNLHPNIHIIRSPNQWLQNTYFWAHHEKFVVIDETFAFIGGTDLCYGRYDTFEHVLRDDAESLLDQNFPGKDYSNARIADFHDLDKPFESMYDRKVIPRMPWHDVQMMTLGEPARDLARHFVQRWNYLLRAKRPSRLTPLLTPPSDLTAEELKSLPMFEILREKSTCETQILRSAGNWSLGLKETECSIQNAYLKLIEQSEHFIYIENQFFITSTVWNGTCVLNKIGDALVDRIVKANQEKKPWKAFILIPLMPGFDSPVDTAEASSLRLIMQFQYQSISRGEHSTFSKLKKLNIDPAQYIQFFSLRKWSTFAPNERLITEQLYVHAKILIADDRRCIIGSANINERSQLGNRDSEVAILIRDTDLIKTKMNGDDYYAGKFPWELRQRLMREHLGCDVDLVEFVEKKFERFEKFAAKNYEKLHTLSKEGDSGNNWSDREMIDSAMIELGYREIFGCKFSPQWKSGHGNSVDDGSTQCGINEKEVGREDENVYEKFFNSVDYGKSSRKRTPLPKHNFASLGLTFNHRAGIENVGIRDHKVLSTDPRLRKNDEHKKEVDGYGPDCWKKESNKKFKADATEQLKEWALNSLASKVLDDKEMIKSEIPEGFSNYLPNEKDLEMYLTDKTVTNRNKWSMLKRICYLQYLSHKLDERKTQRLKKIKDMRRHLSSSTESTRNGSNSLPLNEKSNEGESTNVDQDIEGDEYHRLHEDILKNQELDDGSLDDLLSQIIPKITNFNSGEIDDAKKEELLKLNFIDPYSFEDPLISSFSEGLWFTIALRNTLLYKLVFHCQPDNAVQNWKEYGEFTELEQEFQINQEKLIDLEAENINSTTTNVVDKDREKEKMRKAAELRMKLSGSLLYGFNQKVFDKHTAQRILERIHGHLVIFPTEWLAKEVESRNWIFNSDRLSPMEIYN

Entrez Gene ID

853902

Gene Name

phospholipase D

Gene Symbol

SPO14

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005768	IDA:SGD	C	endosome
GO:0005634	IDA:SGD	C	nucleus
GO:0005628	IDA:SGD	C	prospore membrane
GO:0070290	IEA:UniProtKB-EC	F	N-acylphosphatidylethanolamine-specific phospholipase D activity
GO:0032266	IDA:SGD	F	phosphatidylinositol-3-phosphate binding
GO:0004630	IDA:SGD	F	phospholipase D activity
GO:0031321	IMP:SGD	P	ascospore-type prospore assembly
GO:0000753	IMP:SGD	P	cell morphogenesis involved in conjugation with cellular fusion
GO:0006887	IGI:SGD	P	exocytosis
GO:0016042	IEA:UniProtKB-KW	P	lipid catabolic process
GO:0007126	IEA:UniProtKB-KW	P	meiotic nuclear division
GO:0006644	IDA:SGD	P	phospholipid metabolic process

KEGG Pathway Links

KEGG Pathway ID	Description
ko04144	Endocytosis
sce04144	Endocytosis
ko00565	Ether lipid metabolism
sce00565	Ether lipid metabolism
ko00564	Glycerophospholipid metabolism
sce00564	Glycerophospholipid metabolism
sce01100	Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY-3561	choline biosynthesis
LIPASYN-PWY-1	phospholipids degradation

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618411	Fcgamma receptor (FCGR) dependent phagocytosis
5618125	Glycerophospholipid biosynthesis
5618178	Immune System
5618189	Innate Immune System
5618023	Metabolism
5618091	Metabolism of lipids and lipoproteins
5618126	Phospholipid metabolism
5618410	Role of phospholipids in phagocytosis
5618124	Synthesis of PA
5618596	Synthesis of PG

InterPro Annotations

Accession	Description
IPR015679	Phospholipase D family
IPR016555	Phospholipase D, eukaryota
IPR001736	Phospholipase D/Transphosphatidylase
IPR001683	Phox homologous domain
IPR001849	Pleckstrin homology domain

Entry Information

Gene Name

phospholipase D

Protein Entry

SPO14_YEAST

UniProt ID

P36126

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	A phosphatidylcholine + H(2)O = choline + a phosphatidate.
Enzyme Regulation	Activity is dependent of phosphatidylinositol 4,5-bisphosphate and the regulator SRF1. Inhibited by magnesium
Enzyme Regulation	Activity is dependent of phosphatidylinositol 4,5-bisphosphate and the regulator SRF1. Inhibited by magnesium. {ECO:0000269\|PubMed:21347278}.
Function	Required for meiosis and spore formation. Seems to be involved in the coordinate induction of late meiotic events. PLD activity is induced under sporulation conditions and seems to be necessary to complete the meiotic cycle, but not for vegetative cell growth. {ECO:0000269\|PubMed:1582554, ECO:0000269\|PubMed:21347278}.
Miscellaneous	Present with 49 molecules/cell in log phase SD medium
Miscellaneous	Present with 49 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Sequence Caution	Sequence=AAA74938.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence= ;
Sequence Caution	Sequence=AAA74938.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Similarity	Belongs to the phospholipase D family
Similarity	Belongs to the phospholipase D family. {ECO:0000305}.
Similarity	Contains 1 PH domain
Similarity	Contains 1 PH domain. {ECO:0000305}.
Similarity	Contains 1 PX (phox homology) domain
Similarity	Contains 1 PX (phox homology) domain. {ECO:0000305}.
Similarity	Contains 2 PLD phosphodiesterase domains
Similarity	Contains 2 PLD phosphodiesterase domains. {ECO:0000255\|PROSITE-ProRule:PRU00153}.
Subunit	Interacts with SRF1
Subunit	Interacts with SRF1. {ECO:0000269\|PubMed:21347278}.