Gene/Proteome Database (LMPD)

LMPD ID
LMP007476
Gene ID
856862
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
NEDD4 family E3 ubiquitin-protein ligase
Gene Symbol
RSP5
Synonyms
MDP1; MUT2; NPI1; SMM1; UBY1
Chromosome
V
EC Number
6.3.2.-

Proteins

NEDD4 family E3 ubiquitin-protein ligase
Refseq ID NP_011051
Protein GI 398364769
UniProt ID P39940
mRNA ID NM_001179015
Length 809
MPSSISVKLVAAESLYKRDVFRSPDPFAVLTIDGYQTKSTSAAKKTLNPYWNETFKFDDINENSILTIQVFDQKKFKKKDQGFLGVVNVRVGDVLGHLDEDTATSSGRPREETITRDLKKSNDGMAVSGRLIVVLSKLPSSSPHSQAPSGHTASSSTNTSSTTRTNGHSTSSTRNHSTSHPSRGTAQAVESTLQSGTTAATNTATTSHRSTNSTSSATRQYSSFEDQYGRLPPGWERRTDNFGRTYYVDHNTRTTTWKRPTLDQTEAERGNQLNANTELERRQHRGRTLPGGSSDNSSVTVQVGGGSNIPPVNGAAAAAFAATGGTTSGLGELPSGWEQRFTPEGRAYFVDHNTRTTTWVDPRRQQYIRTYGPTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNVPQYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQEIMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAYDNYTIQINPNSGINPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVYNSLNWMLENSIDGVLDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWKCVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEVQQLPKSHTCFNRVDLPQYVDYDSMKQKLTLAVEETIGFGQE

Gene Information

Entrez Gene ID
856862
Gene Name
NEDD4 family E3 ubiquitin-protein ligase
Gene Symbol
RSP5
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005934 IDA:SGD C cellular bud tip
GO:0005737 IDA:SGD C cytoplasm
GO:0005856 IEA:UniProtKB-KW C cytoskeleton
GO:0010008 IDA:SGD C endosome membrane
GO:0031234 IDA:SGD C extrinsic component of cytoplasmic side of plasma membrane
GO:0005794 IDA:SGD C Golgi apparatus
GO:0005634 IDA:SGD C nucleus
GO:0000151 IPI:SGD C ubiquitin ligase complex
GO:0016874 IEA:UniProtKB-KW F ligase activity
GO:0035091 IDA:SGD F phosphatidylinositol binding
GO:0043130 IDA:SGD F ubiquitin binding
GO:0004842 IDA:SGD F ubiquitin-protein transferase activity
GO:0034644 IMP:SGD P cellular response to UV
GO:0006333 IMP:SGD P chromatin assembly or disassembly
GO:0032511 IMP:SGD P late endosome to vacuole transport via multivesicular body sorting pathway
GO:0007005 IMP:SGD P mitochondrion organization
GO:0045807 IMP:SGD P positive regulation of endocytosis
GO:0045723 IMP:SGD P positive regulation of fatty acid biosynthetic process
GO:0032436 IMP:SGD P positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0048260 IMP:SGD P positive regulation of receptor-mediated endocytosis
GO:0045944 IMP:SGD P positive regulation of transcription from RNA polymerase II promoter
GO:0043161 IPI:SGD P proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0051865 IGI:SGD P protein autoubiquitination
GO:0006513 IDA:SGD P protein monoubiquitination
GO:0000209 IDA:SGD P protein polyubiquitination
GO:0016567 IDA:SGD P protein ubiquitination
GO:0042787 IMP:SGD P protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0032956 IGI:SGD P regulation of actin cytoskeleton organization
GO:0010794 IMP:SGD P regulation of dolichol biosynthetic process
GO:0032443 IMP:SGD P regulation of ergosterol biosynthetic process
GO:0031384 IMP:SGD P regulation of initiation of mating projection growth
GO:0010793 IMP:SGD P regulation of mRNA export from nucleus
GO:0010796 IMP:SGD P regulation of multivesicular body size
GO:0006808 IGI:SGD P regulation of nitrogen utilization
GO:0019220 IGI:SGD P regulation of phosphate metabolic process
GO:0032880 IMP:SGD P regulation of protein localization
GO:2000203 IMP:SGD P regulation of ribosomal large subunit export from nucleus
GO:2000232 IMP:SGD P regulation of rRNA processing
GO:2000238 IMP:SGD P regulation of tRNA export from nucleus
GO:2000235 IMP:SGD P regulation of tRNA processing
GO:0010795 IMP:SGD P regulation of ubiquinone biosynthetic process
GO:0042493 IMP:SGD P response to drug
GO:0034517 IGI:SGD P ribophagy
GO:0070086 IMP:SGD P ubiquitin-dependent endocytosis
GO:0006511 IDA:MGI P ubiquitin-dependent protein catabolic process
GO:0043162 IMP:SGD P ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

KEGG Pathway Links

KEGG Pathway ID Description
ko04144 Endocytosis
sce04144 Endocytosis
ko04120 Ubiquitin mediated proteolysis
sce04120 Ubiquitin mediated proteolysis

Domain Information

InterPro Annotations

Accession Description
IPR000008 C2 domain
IPR024928 E3_ub_ligase_SMURF1
IPR000569 HECT
IPR001202 WW domain

UniProt Annotations

Entry Information

Gene Name
NEDD4 family E3 ubiquitin-protein ligase
Protein Entry
RSP5_YEAST
UniProt ID
P39940
Species
Yeast (S288c)

Comments

Comment Type Description
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other cell surface proteins like GAP1, FUR4, MAL61, PMA1 and STE2. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription, pressure-induced differential regulation of the two tryptophan permeases TAT1 and TAT2 and sorting efficiency into multivesicular bodies. Also acts on RBP1. Plays a role in tolerance to o- dinitrobenzene. Involved in actin cytoskeleton organization and dynamics. Ubiquitinates the LAS17-binding proteins LSB1 and PIN3/LSB2 without directing them for degradation and affects LAS17 levels in a SLA1-dependent and LSB1/2-independent manner. {ECO:0000269|PubMed:12163175, ECO:0000269|PubMed:12821147, ECO:0000269|PubMed:14560004, ECO:0000269|PubMed:15020711, ECO:0000269|PubMed:15247235, ECO:0000269|PubMed:15933713, ECO:0000269|PubMed:16864574, ECO:0000269|PubMed:17079730, ECO:0000269|PubMed:22000681, ECO:0000269|PubMed:7708685, ECO:0000269|PubMed:9858558, ECO:0000269|PubMed:9931424}.
Interaction Q07622:ACK1; NbExp=2; IntAct=EBI-16219, EBI-38674; P80210:ADE12; NbExp=2; IntAct=EBI-16219, EBI-14267; P36117:ALY1; NbExp=4; IntAct=EBI-16219, EBI-26358; P47029:ALY2; NbExp=5; IntAct=EBI-16219, EBI-25974; P18634:ART10; NbExp=4; IntAct=EBI-16219, EBI-27197; P53244:ART5; NbExp=4; IntAct=EBI-16219, EBI-23201; Q05979:BNA5; NbExp=2; IntAct=EBI-16219, EBI-10016; Q00684:CDC14; NbExp=2; IntAct=EBI-16219, EBI-4192; Q12734:CSR2; NbExp=2; IntAct=EBI-16219, EBI-32379; P15202:CTA1; NbExp=2; IntAct=EBI-16219, EBI-4061; P54005:DIA1; NbExp=4; IntAct=EBI-16219, EBI-27668; P53759:DUS1; NbExp=2; IntAct=EBI-16219, EBI-27885; P38167:ECM21; NbExp=2; IntAct=EBI-16219, EBI-21359; P00925:ENO2; NbExp=2; IntAct=EBI-16219, EBI-6475; P36141:FMP46; NbExp=2; IntAct=EBI-16219, EBI-26445; P06738:GPH1; NbExp=2; IntAct=EBI-16219, EBI-13389; P32347:HEM12; NbExp=2; IntAct=EBI-16219, EBI-5711; P53051:IMA1; NbExp=2; IntAct=EBI-16219, EBI-10464; P00817:IPP1; NbExp=2; IntAct=EBI-16219, EBI-9338; Q12502:LDB19; NbExp=3; IntAct=EBI-16219, EBI-2113927; P38998:LYS1; NbExp=3; IntAct=EBI-16219, EBI-10264; P49367:LYS4; NbExp=2; IntAct=EBI-16219, EBI-10276; P36060:MCR1; NbExp=2; IntAct=EBI-16219, EBI-10565; P30952:MLS1; NbExp=3; IntAct=EBI-16219, EBI-10428; Q01560:NPL3; NbExp=2; IntAct=EBI-16219, EBI-12114; P39683:NPT1; NbExp=2; IntAct=EBI-16219, EBI-12218; P10963:PCK1; NbExp=2; IntAct=EBI-16219, EBI-13770; P16862:PFK2; NbExp=3; IntAct=EBI-16219, EBI-9435; P36069:PMU1; NbExp=2; IntAct=EBI-16219, EBI-26862; P25044:PTP1; NbExp=2; IntAct=EBI-16219, EBI-14183; P11154:PYC1; NbExp=2; IntAct=EBI-16219, EBI-14358; P38212:RCR1; NbExp=3; IntAct=EBI-16219, EBI-21381; Q03446:RCR2; NbExp=2; IntAct=EBI-16219, EBI-18180; Q00453:RGM1; NbExp=2; IntAct=EBI-16219, EBI-15073; Q02805:ROD1; NbExp=3; IntAct=EBI-16219, EBI-15679; P43602:ROG3; NbExp=3; IntAct=EBI-16219, EBI-22976; P20436:RPB8; NbExp=3; IntAct=EBI-16219, EBI-15794; P14359:SNA3; NbExp=2; IntAct=EBI-16219, EBI-26122; Q07549:SNA4; NbExp=2; IntAct=EBI-16219, EBI-22078; P39015:STM1; NbExp=3; IntAct=EBI-16219, EBI-11238; Q07748:THI13; NbExp=2; IntAct=EBI-16219, EBI-36080; P43534:THI5; NbExp=2; IntAct=EBI-16219, EBI-19221; P23254:TKL1; NbExp=2; IntAct=EBI-16219, EBI-19291; Q08919:TRE1; NbExp=3; IntAct=EBI-16219, EBI-31915; Q12162:TY1A-PL; NbExp=2; IntAct=EBI-16219, EBI-36658; Q12472:TY2B-DR1; NbExp=2; IntAct=EBI-16219, EBI-35737; P33296:UBC6; NbExp=2; IntAct=EBI-16219, EBI-19745; P38081:YBR056W; NbExp=3; IntAct=EBI-16219, EBI-21453; P25561:YCL021W; NbExp=3; IntAct=EBI-16219, EBI-21696; P38835:YHR131C; NbExp=3; IntAct=EBI-16219, EBI-24724; P53108:YIP5; NbExp=2; IntAct=EBI-16219, EBI-24051; P40892:YJL218W; NbExp=2; IntAct=EBI-16219, EBI-26263; P47137:YJR096W; NbExp=3; IntAct=EBI-16219, EBI-25572; P36140:YKR047W; NbExp=2; IntAct=EBI-16219, EBI-26441;
Miscellaneous A cysteine residue is required for ubiquitin- thioester formation.
Pathway Protein modification; protein ubiquitination.
Ptm The ubiquitination appears to be the result of an intramolecular transfer of ubiquitin.
Similarity Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}.
Similarity Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain
Similarity Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. {ECO:0000255|PROSITE-ProRule:PRU00104}.
Similarity Contains 3 WW domains. {ECO:0000255|PROSITE- ProRule:PRU00224}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:22000681}. Nucleus {ECO:0000305}. Cytoplasm, cytoskeleton, actin patch {ECO:0000269|PubMed:22000681}.
Subcellular Location Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, actin patch .
Subunit Component of the RSP5-BUL1/2 ubiquitin ligase complex composed of at least RSP5 and BUL1 or BUL2. Forms also a ternary complex with RUP1 and UBP2. Interacts (via WW domains) with LSB1, PIN3/LSB2 and RCR1 (via PY motifs). Interacts with HSE1, LAS17, ROG3, ROD1 and RVS167. {ECO:0000269|PubMed:12163175, ECO:0000269|PubMed:15086794, ECO:0000269|PubMed:15933713, ECO:0000269|PubMed:17079730, ECO:0000269|PubMed:17213653, ECO:0000269|PubMed:21399621, ECO:0000269|PubMed:22000681, ECO:0000269|PubMed:8668140, ECO:0000269|PubMed:9931424}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007476 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
398364769 RefSeq NP_011051 809 NEDD4 family E3 ubiquitin-protein ligase

Identical Sequences to LMP007476 proteins

Reference Database Accession Length Protein Name

Related Sequences to LMP007476 proteins

Reference Database Accession Length Protein Name