LIPID MAPS

LMPD ID

LMP007477

Gene ID

850964

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

sphinganine kinase LCB5

Gene Symbol

LCB5

Synonyms

-

Chromosome

XII

EC Number

2.7.1.91

sphinganine kinase LCB5
Refseq ID	NP_013361
Protein GI	6323289
UniProt ID	Q06147
mRNA ID	NM_001182147
Length	687
MTLKPSKRRKGRSRHSRKKQITSAILTEEGIMIKAKPSSPYTYANRMADKRSRSSIDNISRTSFQSNISRTSFQSNSDNNSIFETASLISCVTCLSDTDTIDRSETSTTDTSKDDLSANPKLHYPSVNGQLPANTVIPYGRILDARYIEKEPLHYYDANSSPSSPLSSSMSNISEKCDLDELESSQKKERKGNSLSRGSNSSSSLLTSRSPFTKLVEVIFARPRRHDVVPKRVSLYIDYKPHSSSHLKEEDDLVEEILKRSYKNTRRNKSIFVIINPFGGKGKAKKLFMTKAKPLLLASRCSIEVVYTKYPGHAIEIAREMDIDKYDTIACASGDGIPHEVINGLYQRPDHVKAFNNIAITEIPCGSGNAMSVSCHWTNNPSYSTLCLIKSIETRIDLMCCSQPSYAREHPKLSFLSQTYGLIAETDINTEFIRWMGPARFELGVAFNIIQKKKYPCEIYVKYAAKSKNELKNHYLEHKNKGSLEFQHITMNKDNEDCDNYNYENEYETENEDEDEDADADDEDSHLISRDLADSSADQIKEEDFKIKYPLDEGIPSDWERLDPNISNNLGIFYTGKMPYVAADTKFFPAALPSDGTMDMVITDARTSLTRMAPILLGLDKGSHVLQPEVLHSKILAYKIIPKLGNGLFSVDGEKFPLEPLQVEIMPRLCKTLLRNGRYVDTDFDSM

Entrez Gene ID

850964

Gene Name

sphinganine kinase LCB5

Gene Symbol

LCB5

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005794	IDA:SGD	C	Golgi apparatus
GO:0016020	IDA:SGD	C	membrane
GO:0005524	IEA:UniProtKB-KW	F	ATP binding
GO:0017050	IDA:SGD	F	D-erythro-sphingosine kinase activity
GO:0003951	IEA:InterPro	F	NAD+ kinase activity
GO:0004143	IEA:InterPro	F	diacylglycerol kinase activity
GO:0008481	IEA:UniProtKB-EC	F	sphinganine kinase activity
GO:0019722	IMP:SGD	P	calcium-mediated signaling
GO:0007205	IEA:InterPro	P	protein kinase C-activating G-protein coupled receptor signaling pathway
GO:0009408	IDA:SGD	P	response to heat
GO:0006665	IDA:SGD	P	sphingolipid metabolic process

KEGG Pathway Links

KEGG Pathway ID	Description
sce01100	Metabolic pathways
ko00600	Sphingolipid metabolism
sce00600	Sphingolipid metabolism
M00100	Sphingosine degradation
sce_M00100	Sphingosine degradation

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY-7119	sphingolipid recycling and degradation (yeast)
PWY-7119	sphingolipid recycling and degradation (yeast)

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618486	Association of TriC/CCT with target proteins during biosynthesis
5618480	Chaperonin-mediated protein folding
5618602	Glycosphingolipid metabolism
5618023	Metabolism
5618091	Metabolism of lipids and lipoproteins
5618074	Metabolism of proteins
5618481	Protein folding
5618100	Signal Transduction
5618195	Signaling by VEGF
5618508	Sphingolipid de novo biosynthesis
5618509	Sphingolipid metabolism
5618194	VEGFA-VEGFR2 Pathway
5618193	VEGFR2 mediated cell proliferation

InterPro Annotations

Accession	Description
IPR016064	ATP-NAD kinase-like domain
IPR001206	Diacylglycerol kinase, catalytic domain

Entry Information

Gene Name

sphinganine kinase LCB5

Protein Entry

LCB5_YEAST

UniProt ID

Q06147

Species

Yeast (S288c)

Comments

Comment Type	Description
Biophysicochemical Properties	Kinetic parameters: KM=2 uM for dihydrosphingosine {ECO:0000269\|PubMed:9677363};
Biophysicochemical Properties	Kinetic parameters: KM=2 uM for dihydrosphingosine ;
Catalytic Activity	ATP + phytosphingosine = ADP + phytosphingosine 1-phosphate.
Catalytic Activity	ATP + sphinganine = ADP + sphinganine 1- phosphate.
Function	Catalyzes the phosphorylation of the sphingoid long chain bases dihydrosphingosine (DHS or sphinganine) and phytosphingosine (PHS) to form dihydrosphingosine 1-phosphate (DHS-1P) and phytosphingosine 1-phosphate (PHS-1P) respectively. Redundant to LCB4, is only responsible for few percent of the total activity. Involved in the biosynthesis of sphingolipids and ceramides. Involved in heat-induced transient cell cycle arrest. Accumulation of phosphorylated sphingoid long chain bases (LCBPs) stimulates calcium influx and activates calcineurin signaling. Involved in heat-stress resistance. {ECO:0000269\|PubMed:11056159, ECO:0000269\|PubMed:11102354, ECO:0000269\|PubMed:11278643, ECO:0000269\|PubMed:12493772, ECO:0000269\|PubMed:9677363}.
Miscellaneous	Present with 1760 molecules/cell in log phase SD medium
Miscellaneous	Present with 1760 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity	Contains 1 DAGKc domain. {ECO:0000255\|PROSITE- ProRule:PRU00783}.
Subcellular Location	Golgi apparatus membrane ; Peripheral membrane protein .
Subcellular Location	Golgi apparatus membrane {ECO:0000269\|PubMed:12493772}; Peripheral membrane protein {ECO:0000269\|PubMed:12493772}.