LIPID MAPS

LMPD ID

LMP007479

Gene ID

853455

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

Ost1p

Gene Symbol

OST1

Synonyms

NLT1

Chromosome

X

EC Number

2.4.99.18

Ost1p
Refseq ID	NP_012532
Protein GI	398364715
UniProt ID	P41543
mRNA ID	NM_001181436
Length	476
MRQVWFSWIVGLFLCFFNVSSAAQYEPPATWENVDYKRTIDVSNAYISETIEITIKNIASEPATEYFTAFESGIFSKVSFFSAYFTNEATFLNSQLLANSTTAPGDDGESEIRYGIIQFPNAISPQEEVSLVIKSFYNTVGIPYPEHVGMSEEQHLLWETNRLPLSAYDTKKASFTLIGSSSFEEYHPPNDESLLGKANGNSFEFGPWEDIPRFSSNETLAIVYSHNAPLNQVVNLRRDIWLSHWASTIQFEEYYELTNKAAKLSKGFSRLELMKQIQTQNMRQTHFVTVLDMLLPEGATDHYFTDLVGLVSTSHAERDHFFIRPRFPIFGGWNYNFTVGWTNKLSDFLHVSSGSDEKFVASIPILNGPPDTVYDNVELSVFLPEGAEIFDIDSPVPFTNVSIETQKSYFDLNKGHVKLTFSYRNLISQVANGQVLIKYDYPKSSFFKKPLSIACYIFTALMGVFVLKTLNMNVTN

Entrez Gene ID

853455

Gene Name

Ost1p

Gene Symbol

OST1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0008250	IPI:SGD	C	oligosaccharyltransferase complex
GO:0004579	IDA:SGD	F	dolichyl-diphosphooligosaccharide-protein glycotransferase activity
GO:0042802	IPI:IntAct	F	identical protein binding
GO:0006487	IDA:SGD	P	protein N-linked glycosylation
GO:0018279	IPI:SGD	P	protein N-linked glycosylation via asparagine

KEGG Pathway Links

KEGG Pathway ID	Description
sce01100	Metabolic pathways
ko00510	N-Glycan biosynthesis
sce00510	N-Glycan biosynthesis
M00072	N-glycosylation by oligosaccharyltransferase
sce_M00072	N-glycosylation by oligosaccharyltransferase
ko04141	Protein processing in endoplasmic reticulum
sce04141	Protein processing in endoplasmic reticulum
ko00513	Various types of N-glycan biosynthesis
sce00513	Various types of N-glycan biosynthesis

InterPro Annotations

Accession	Description
IPR007676	Ribophorin_I

Entry Information

Gene Name

Ost1p

Protein Entry

OST1_YEAST

UniProt ID

P41543

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
Function	Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Interaction	Self; NbExp=2; IntAct=EBI-12651, EBI-12651; P35179:SSS1; NbExp=2; IntAct=EBI-12651, EBI-16406;
Miscellaneous	Present with 11900 molecules/cell in log phase SD medium
Miscellaneous	Present with 11900 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Protein modification; protein glycosylation.
Similarity	Belongs to the OST1 family
Similarity	Belongs to the OST1 family. {ECO:0000305}.
Subcellular Location	Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Subunit	Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST1 and OST5 probably form a subcomplex. OST1 may interact directly with OST2 OST3, OST5, OST6, WBP1 AND SWP1. Interacts with SEC61, SBH1 and SSS1. {ECO:0000269\|PubMed:15831493, ECO:0000269\|PubMed:15886282, ECO:0000269\|PubMed:8175708, ECO:0000269\|PubMed:9405463}.