LIPID MAPS

LMPD ID

LMP007486

Gene ID

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

ketol-acid reductoisomerase

Gene Symbol

ILV5

Synonyms

-

Chromosome

XII

EC Number

1.1.1.86

ketol-acid reductoisomerase
Refseq ID	NP_013459
Protein GI	6323387
UniProt ID	P06168
mRNA ID	NM_001182244
Length	395
MLRTQAARLICNSRVITAKRTFALATRAAAYSRPAARFVKPMITTRGLKQINFGGTVETVYERADWPREKLLDYFKNDTFALIGYGSQGYGQGLNLRDNGLNVIIGVRKDGASWKAAIEDGWVPGKNLFTVEDAIKRGSYVMNLLSDAAQSETWPAIKPLLTKGKTLYFSHGFSPVFKDLTHVEPPKDLDVILVAPKGSGRTVRSLFKEGRGINSSYAVWNDVTGKAHEKAQALAVAIGSGYVYQTTFEREVNSDLYGERGCLMGGIHGMFLAQYDVLRENGHSPSEAFNETVEEATQSLYPLIGKYGMDYMYDACSTTARRGALDWYPIFKNALKPVFQDLYESTKNGTETKRSLEFNSQPDYREKLEKELDTIRNMEIWKVGKEVRKLRPENQ

Entrez Gene ID

Gene Name

ketol-acid reductoisomerase

Gene Symbol

ILV5

Species

Saccharomyces cerevisiae S288c

GO ID	Source	Type	Description
GO:0042645	IDA:SGD	C	mitochondrial nucleoid
GO:0005739	IDA:SGD	C	mitochondrion
GO:0050662	IEA:InterPro	F	coenzyme binding
GO:0003690	IDA:SGD	F	double-stranded DNA binding
GO:0004455	IMP:SGD	F	ketol-acid reductoisomerase activity
GO:0046872	IEA:UniProtKB-KW	F	metal ion binding
GO:0009082	IMP:SGD	P	branched-chain amino acid biosynthetic process
GO:0009097	IEA:UniProtKB-UniPathway	P	isoleucine biosynthetic process
GO:0000002	IMP:SGD	P	mitochondrial genome maintenance
GO:0009099	IEA:UniProtKB-UniPathway	P	valine biosynthetic process

KEGG Pathway ID	Description
ko01210	2-Oxocarboxylic acid metabolism
sce01210	2-Oxocarboxylic acid metabolism
ko01230	Biosynthesis of amino acids
sce01230	Biosynthesis of amino acids
sce01110	Biosynthesis of secondary metabolites
M00570	Isoleucine biosynthesis, threonine => 2-oxobutanoate => isoleucine
sce_M00570	Isoleucine biosynthesis, threonine => 2-oxobutanoate => isoleucine
sce01100	Metabolic pathways
ko00770	Pantothenate and CoA biosynthesis
sce00770	Pantothenate and CoA biosynthesis
M00019	Valine/isoleucine biosynthesis, pyruvate => valine / 2-oxobutanoate => isoleucine
sce_M00019	Valine/isoleucine biosynthesis, pyruvate => valine / 2-oxobutanoate => isoleucine
ko00290	Valine, leucine and isoleucine biosynthesis
sce00290	Valine, leucine and isoleucine biosynthesis

BIOCYC Pathway ID	Description
ILEUSYN-PWY	isoleucine biosynthesis
BRANCHED-CHAIN-AA-SYN-PWY	superpathway of leucine, valine, and isoleucine biosynthesis
VALSYN-PWY	valine biosynthesis

Accession	Description
IPR008927	6-phosphogluconate dehydrogenase, C-terminal-like
IPR013023	AcH_isomrdctse
IPR000506	AcH_isomrdctse_C
IPR013328	Dehydrogenase, multihelical
IPR013116	IlvN
IPR016207	KetolA_reductoisomerase_fun

Gene Name

ketol-acid reductoisomerase

Protein Entry

ILV5_YEAST

UniProt ID

Species

Yeast (S288c)

Comment Type	Description
Catalytic Activity	(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Catalytic Activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Binds 2 magnesium ions per subunit. {ECO:0000250};
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ; Note=Binds 2 magnesium ions per subunit. ;
Miscellaneous	Present with 883000 molecules/cell in log phase SD medium
Miscellaneous	Present with 883000 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L- isoleucine from 2-oxobutanoate: step 2/4.
Pathway	Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Similarity	Belongs to the ketol-acid reductoisomerase family
Similarity	Belongs to the ketol-acid reductoisomerase family. {ECO:0000305}.
Subcellular Location	Mitochondrion.