Gene/Proteome Database (LMPD)
Proteins
| Ubx2p | |
|---|---|
| Refseq ID | NP_013699 |
| Protein GI | 6323628 |
| UniProt ID | Q04228 |
| mRNA ID | NM_001182371 |
| Length | 584 |
| RefSeq Status | PROVISIONAL |
| MPVVNHEDSEFHLSHTEEDKLNEFQVITNFPPEDLPDVVRLLRNHGWQLEPALSRYFDGEWKGEPDQMGEPTQTSTPMAETLVPPALGPRPLLFTASLPVVRPLPANFRNDFRTIGLNGRSNTVWSMFESFSYDGNPFLFILLLIPRIINRLSATIFTFFCTLLSLHSISGGGNSGKPKISKVPKAPTRETHIPLAEILGDTKDKDAFCELKSFKPDISFNEALRIAKEEFKFMLLILVGDTYDTDTDTVDVNSKLLLEKILLNKKTLQYLRKIDNDLIIYLKCVHELEPWLVARQLGVRNTPEIFLIANVANKASHSETLPSQRLSILGKLKVNSLNRFLQSLTNVVEKYTPELVVNKTEMHELRMSREIKKLQEDAYKKSLEMDRIKAIEKEKSLKHAQDLKLNSTARQLKWLKACIDEIQPFETTGKQATLQFRTSSGKRFVKKFPSMTTLYQIYQSIGCHIYLAVYSSDPAEWSNALQDKIRQLSADDDMLCFKEGQLETATATTIEELGHIINNELTSFDLERGKLEFDFELVSPFPKYTVHPNEHMSVDQVPQLWPNGSLLVEALDEEDEEDEENEEQ | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0000837 | IDA:SGD | C | Doa10p ubiquitin ligase complex |
| GO:0000839 | IDA:SGD | C | Hrd1p ubiquitin ligase ERAD-L complex |
| GO:0030176 | IDA:SGD | C | integral component of endoplasmic reticulum membrane |
| GO:0005887 | IDA:SGD | C | integral component of plasma membrane |
| GO:0005811 | IDA:SGD | C | lipid particle |
| GO:0030674 | IPI:SGD | F | protein binding, bridging |
| GO:0030433 | IMP:SGD | P | ER-associated ubiquitin-dependent protein catabolic process |
| GO:0034389 | IMP:SGD | P | lipid particle organization |
| GO:0043161 | IMP:SGD | P | proteasome-mediated ubiquitin-dependent protein catabolic process |
KEGG Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR001012 | UBX domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Function | Integral endoplasmic reticulum membrane protein that coordinates the assembly of the ER-associated protein degradation (ERAD) machinery at the ER membrane. Mediates binding of CDC48 to the E3 ubiquitin ligases SSM4/DOA10 and HRD1, and to ERAD substrates. Component of the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains. ERAD-C substrates are ubiquitinated through DOA10 in conjunction with the E2 ubiquitin-conjugating enzymes UBC6 and UBC7-CUE1. Also component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). ERAD-L substrates are ubiquitinated through HRD1 in conjunction with the E2 ubiquitin-conjugating enzymes UBC1 and UBC7-CUE1. Ubiquitinated substrates are then removed to the cytosol via the action of the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex and targeted to the proteasome. {ECO:0000269|PubMed:15258615, ECO:0000269|PubMed:16179952, ECO:0000269|PubMed:16179953, ECO:0000269|PubMed:16873066}. |
| Interaction | P25694:CDC48; NbExp=11; IntAct=EBI-27730, EBI-4308; P40318:SSM4; NbExp=5; IntAct=EBI-27730, EBI-18208; P53044:UFD1; NbExp=5; IntAct=EBI-27730, EBI-19997; Q99220:YOS9; NbExp=3; IntAct=EBI-27730, EBI-34938; |
| Miscellaneous | Present with 12600 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity | Contains 1 UBX domain. {ECO:0000305}. |
| Subcellular Location | Endoplasmic reticulum membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Subunit | Component of the DOA10 complex which contains SSM4/DOA10, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the membrane integral E3 ligase SSM4/DOA10 and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recuited by UBX2/SEL1. Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, KAR2, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factors KAR2 and YOS9, and the heterotrimeric UFD1- NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1. Interacts with CDC48. {ECO:0000269|PubMed:15258615, ECO:0000269|PubMed:16179952, ECO:0000269|PubMed:16179953, ECO:0000269|PubMed:16873066}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007510 (as displayed in Record Overview)
Identical Sequences to LMP007510 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6323628 | EMBL | CAA89939.1 | 584 | unknown [Saccharomyces cerevisiae] |
| GI:6323628 | GenBank | EIW08245.1 | 584 | Ubx2p [Saccharomyces cerevisiae CEN.PK113-7D] |
| GI:6323628 | SwissProt | Q04228.1 | 584 | RecName: Full=UBX domain-containing protein 2; AltName: Full=Secretion lowering protein 1 [Saccharomyces cerevisiae S288c] |
| GI:6323628 | Third Party Genbank | DAA09885.1 | 584 | TPA: Ubx2p [Saccharomyces cerevisiae S288c] |
Related Sequences to LMP007510 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6323628 | DBBJ | GAA25432.1 | 584 | K7_Sel1p [Saccharomyces cerevisiae Kyokai no. 7] |
| GI:6323628 | GenBank | EDN64379.1 | 584 | conserved protein [Saccharomyces cerevisiae YJM789] |
| GI:6323628 | GenBank | EDV11491.1 | 584 | UBX domain-containing protein 2 [Saccharomyces cerevisiae RM11-1a] |
| GI:6323628 | GenBank | EGA85300.1 | 584 | Ubx2p [Saccharomyces cerevisiae VL3] |
| GI:6323628 | GenBank | EWG83919.1 | 584 | Ubx2p [Saccharomyces cerevisiae R008] |
| GI:6323628 | GenBank | EWG94336.1 | 584 | Ubx2p [Saccharomyces cerevisiae R103] |