LIPID MAPS

LMPD ID

LMP007534

Gene ID

854251

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

Lpx1p

Gene Symbol

LPX1

Synonyms

-

Chromosome

XV

EC Number

3.1.1.-

Lpx1p
Refseq ID	NP_014727
Protein GI	6324658
UniProt ID	Q12405
mRNA ID	NM_001183503
Length	387
MEQNRFKKETKTCSASWPRAPQSTLCATDRLELTYDVYTSAERQRRSRTATRLNLVFLHGSGMSKVVWEYYLPRLVAADAEGNYAIDKVLLIDQVNHGDSAVRNRGRLGTNFNWIDGARDVLKIATCELGSIDSHPALNVVIGHSMGGFQALACDVLQPNLFHLLILIEPVVITRKAIGAGRPGLPPDSPQIPENLYNSLRLKTCDHFANESEYVKYMRNGSFFTNAHSQILQNIIDFERTKASGDDEDGGPVRTKMEQAQNLLCYMNMQTFAPFLISNVKFVRKRTIHIVGARSNWCPPQNQLFLQKTLQNYHLDVIPGGSHLVNVEAPDLVIERINHHIHEFVLTSPLQSSHIPQLTLEERAVMFDRAFDSFKNEALVKTTKQKL

Entrez Gene ID

854251

Gene Name

Lpx1p

Gene Symbol

LPX1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005782	IDA:SGD	C	peroxisomal matrix
GO:0016298	IDA:SGD	F	lipase activity
GO:0004806	IDA:SGD	F	triglyceride lipase activity
GO:0007031	IEP:SGD	P	peroxisome organization
GO:0019433	IGI:SGD	P	triglyceride catabolic process

InterPro Annotations

Accession	Description
IPR029058	Alpha/Beta hydrolase fold

Entry Information

Gene Name

Lpx1p

Protein Entry

LPX1_YEAST

UniProt ID

Q12405

Species

Yeast (S288c)

Comments

Comment Type	Description
Biophysicochemical Properties	Kinetic parameters: KM=6.3 uM for p-nitrophenyl butyrate (PNB) {ECO:0000269\|PubMed:18199283}; Vmax=5.6 pmol/h/ug enzyme toward 1,2-dioleoyl-3-(pyren-1- yl)decanoyl-rac-glycerol (DPG) {ECO:0000269\|PubMed:18199283}; Vmax=7.9 pmol/h/ug enzyme toward 1,2-bis-(4,4-difluoro-5,7- dimethyl-4-bora-3a,4a-diaza-sindacene-3-undecanoyl)-sn-glycero- 3-phosphocholine (BPC) {ECO:0000269\|PubMed:18199283};
Biophysicochemical Properties	Kinetic parameters: KM=6.3 uM for p-nitrophenyl butyrate (PNB) ; Vmax=5.6 pmol/h/ug enzyme toward 1,2-dioleoyl-3-(pyren-1- yl)decanoyl-rac-glycerol (DPG) ; Vmax=7.9 pmol/h/ug enzyme toward 1,2-bis-(4,4-difluoro-5,7- dimethyl-4-bora-3a,4a-diaza-sindacene-3-undecanoyl)-sn-glycero- 3-phosphocholine (BPC) ;
Disruption Phenotype	Aberrant morphology characterized by intraperoxisomal vesicles or invaginations
Disruption Phenotype	Aberrant morphology characterized by intraperoxisomal vesicles or invaginations. {ECO:0000269\|PubMed:18199283}.
Function	Has acyl esterase, lipase and phospholipase A activity
Function	Has acyl esterase, lipase and phospholipase A activity. {ECO:0000269\|PubMed:18199283}.
Induction	By oleic acid. Transcriptionally up-regulated by YRM1 along with genes involved in multidrug resistance. Expression is also dependent on RSF1 and RSF2 for transcriptional induction during growth on glycerol-based medium. {ECO:0000269\|PubMed:12135984, ECO:0000269\|PubMed:14512416, ECO:0000269\|PubMed:18199283, ECO:0000269\|PubMed:19235764}.
Miscellaneous	Present with 2350 molecules/cell in log phase SD medium
Miscellaneous	Present with 2350 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Subcellular Location	Peroxisome matrix . Note=Peroxisomal import is dependent on the peroxisomal targeting signal type 1 (PTS1) receptor PEX5 and on self-interaction.
Subcellular Location	Peroxisome matrix {ECO:0000269\|PubMed:12135984, ECO:0000269\|PubMed:18199283}. Note=Peroxisomal import is dependent on the peroxisomal targeting signal type 1 (PTS1) receptor PEX5 and on self-interaction.