Gene/Proteome Database (LMPD)

LMPD ID
LMP007548
Gene ID
948541
Species
Escherichia coli K-12 (E. coli)
Gene Name
glycerol-3-phosphate O-acyltransferase
Gene Symbol
plsB
Synonyms
ECK4033; JW4001
Summary
HT_Cmplx46_Mem: [More information is available at EcoGene: EG10740]. Membrane-bound glycerol-3-phosphate acyltransferase catalyzes the first step in phospholipid biosynthesis. [More information is available at EcoCyc: EG10740].
Orthologs
View orthologs and multiple alignments for plsB

Proteins

glycerol-3-phosphate O-acyltransferase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_418465
Protein GI 90111678
UniProt ID P0A7A7
Length 807
RefSeq Status REVIEWED
MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLGRDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLTIESATQGEG

Gene Information

Entrez Gene ID
948541
Gene Name
glycerol-3-phosphate O-acyltransferase
Gene Symbol
plsB
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005887 IDA:EcoliWiki C integral component of plasma membrane
GO:0004366 IMP:EcoliWiki F glycerol-3-phosphate O-acyltransferase activity
GO:0016024 IEA:UniProtKB-UniPathway P CDP-diacylglycerol biosynthetic process
GO:0006631 IMP:EcoliWiki P fatty acid metabolic process
GO:0008654 IMP:EcoliWiki P phospholipid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco00561 Glycerolipid metabolism
ko00561 Glycerolipid metabolism
eco00564 Glycerophospholipid metabolism
ko00564 Glycerophospholipid metabolism
eco01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-5667 CDP-diacylglycerol biosynthesis I
PWY-5667 CDP-diacylglycerol biosynthesis I
PWY-5667 CDP-diacylglycerol biosynthesis I
PWY0-1319 CDP-diacylglycerol biosynthesis II
PWY0-1319 CDP-diacylglycerol biosynthesis II
PWY0-1319 CDP-diacylglycerol biosynthesis II
PWY4FS-7 phosphatidylglycerol biosynthesis I (plastidic)
PWY4FS-8 phosphatidylglycerol biosynthesis II (non-plastidic)
PHOSLIPSYN-PWY phospholipid biosynthesis I
PHOSLIPSYN-PWY phospholipid biosynthesis I
PHOSLIPSYN-PWY phospholipid biosynthesis I

Domain Information

InterPro Annotations

Accession Description
IPR022284 Glycerol-3-phosphate O-acyltransferase/Dihydroxyacetone phosphate acyltransferase
IPR028354 Glycerol-3-phosphate acyltransferase, PlsB
IPR002123 Phospholipid/glycerol acyltransferase

UniProt Annotations

Entry Information

Gene Name
glycerol-3-phosphate O-acyltransferase
Protein Entry
PLSB_ECOLI
UniProt ID
P0A7A7
Species
E. coli

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=49 uM for glycerol-3-phosphate {ECO:0000269|PubMed:10231527, ECO:0000269|PubMed:7026564}; Vmax=13.1 nmol/min/mg enzyme with glycerol-3-phosphate as substrate {ECO:0000269|PubMed:10231527, ECO:0000269|PubMed:7026564}; pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:10231527, ECO:0000269|PubMed:7026564};
Catalytic Activity Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1- acyl-sn-glycerol 3-phosphate.
Domain The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
Function Catalyzes the transfer of an acyl group from acyl-ACP to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme can utilize either acyl-CoA or acyl-ACP as the fatty acyl donor. {ECO:0000269|PubMed:17645809, ECO:0000269|PubMed:6997313}.
Interaction P0A8Z3:ybgC; NbExp=3; IntAct=EBI-542961, EBI-543276;
Pathway Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Sequence Caution Sequence=AAC43135.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
Similarity Belongs to the GPAT/DAPAT family. {ECO:0000305}.
Subcellular Location Cell inner membrane {ECO:0000269|PubMed:16294310, ECO:0000269|PubMed:4943977}; Peripheral membrane protein {ECO:0000269|PubMed:16294310, ECO:0000269|PubMed:4943977}; Cytoplasmic side {ECO:0000269|PubMed:16294310, ECO:0000269|PubMed:4943977}.
Subunit Interacts with ACP, YbgC and PssA, forming altogether a complex at the inner membrane. {ECO:0000269|PubMed:16294310}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007548 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
90111678 RefSeq NP_418465 807 glycerol-3-phosphate O-acyltransferase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007548 proteins

Reference Database Accession Length Protein Name
GI:90111678 GenBank KHJ00703.1 807 glycerol-3-phosphate acyltransferase [Escherichia coli]
GI:90111678 GenBank KHJ07229.1 807 glycerol-3-phosphate acyltransferase [Escherichia coli]
GI:90111678 GenBank KHJ11952.1 807 glycerol-3-phosphate acyltransferase [Escherichia coli]
GI:90111678 GenBank KHJ20901.1 807 glycerol-3-phosphate acyltransferase [Escherichia coli]
GI:90111678 GenBank KHJ26014.1 807 glycerol-3-phosphate acyltransferase [Escherichia coli]
GI:90111678 gnl IGS 807 glycerol-3-phosphate O-acyltransferase [Escherichia coli ER2796]

Related Sequences to LMP007548 proteins

Reference Database Accession Length Protein Name
GI:90111678 DBBJ BAG79857.1 827 glycerol-3-phosphate acyltransferase [Escherichia coli SE11]
GI:90111678 GenBank EIE55376.1 827 glycerol-3-phosphate O-acyltransferase [Escherichia coli AI27]
GI:90111678 GenBank EIG90612.1 827 glycerol-3-phosphate O-acyltransferase [Escherichia coli 97.0246]
GI:90111678 gnl zhongguo 827 Glycerol-3-phosphate acyltransferase [Escherichia coli P12b]
GI:90111678 RefSeq YP_002295608.1 827 glycerol-3-phosphate acyltransferase [Escherichia coli SE11]
GI:90111678 SwissProt Q1R3P5.1 827 RecName: Full=Glycerol-3-phosphate acyltransferase; Short=GPAT [Escherichia coli UTI89]