Gene/Proteome Database (LMPD)

LMPD ID
LMP007552
Gene ID
946626
Species
Escherichia coli K-12 (E. coli)
Gene Name
phosphatidylglycerol kinase, metal-dependent
Gene Symbol
yegS
Synonyms
ECK2082; JW2070
Summary
Lipid kinase activity demonstrated in vitro; physiological role uncertain (Bakali, 2007). [More information is available at EcoGene: EG14367]. YegS is a lipid kinase with phosphatidylglycerol kinase activity in vitro . [More information is available at EcoCyc: G7123].
Orthologs
View orthologs and multiple alignments for yegS

Proteins

phosphatidylglycerol kinase, metal-dependent [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_416590
Protein GI 16130026
UniProt ID P76407
Length 299
RefSeq Status REVIEWED
MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKGDAARYVEEARKFGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATSVGIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALGSVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALINDGLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHDITFNLDGEPLSGQNFHIEILPAALRCRLPPDCPLLR

Gene Information

Entrez Gene ID
946626
Gene Name
phosphatidylglycerol kinase, metal-dependent
Gene Symbol
yegS
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-HAMAP C cytoplasm
GO:0005524 IEA:UniProtKB-HAMAP F ATP binding
GO:0003951 IEA:InterPro F NAD+ kinase activity
GO:0004143 IEA:InterPro F diacylglycerol kinase activity
GO:0001727 IDA:EcoCyc F lipid kinase activity
GO:0000287 IEA:UniProtKB-HAMAP F magnesium ion binding
GO:0046834 IDA:GOC P lipid phosphorylation
GO:0008654 IEA:UniProtKB-HAMAP P phospholipid biosynthetic process
GO:0007205 IEA:InterPro P protein kinase C-activating G-protein coupled receptor signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR016064 ATP-NAD kinase-like domain
IPR001206 Diacylglycerol kinase, catalytic domain
IPR005218 Diacylglycerol/lipid kinase
IPR022433 Lip_kinase_YegS

UniProt Annotations

Entry Information

Gene Name
phosphatidylglycerol kinase, metal-dependent
Protein Entry
YEGS_ECOLI
UniProt ID
P76407
Species
E. coli

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=0.7 mM for phosphatidylglycerol; Vmax=29.7 nmol/min/mg enzyme; pH dependence: Optimum pH is 7.5. Active from pH 5 to 9.;
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:17351295}; Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:17351295}; Note=Binds 1 Mg(2+) ion per subunit. Ca(2+) may be able to substitute. {ECO:0000269|PubMed:17351295};
Function In vitro phosphorylates phosphatidylglycerol but not diacylglycerol; the in vivo substrate is unknown.
Interaction P0ABH7:gltA; NbExp=2; IntAct=EBI-1127478, EBI-547808;
Similarity Belongs to the diacylglycerol/lipid kinase family. YegS lipid kinase subfamily. {ECO:0000305}.
Similarity Contains 1 DAGKc domain. {ECO:0000305}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:17351295}.
Subunit Monomer. {ECO:0000269|PubMed:16511327, ECO:0000269|PubMed:17351295}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007552 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16130026 RefSeq NP_416590 299 phosphatidylglycerol kinase, metal-dependent [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007552 proteins

Reference Database Accession Length Protein Name
GI:16130026 EMBL CDY59446.1 299 lipid kinase [Escherichia coli]
GI:16130026 EMBL CDZ20907.1 299 lipid kinase [Escherichia coli]
GI:16130026 GenBank KGA85629.1 299 lipid kinase [Escherichia coli]
GI:16130026 GenBank KGL68946.1 299 lipid kinase [Escherichia coli NCTC 50110]
GI:16130026 GenBank KHH20582.1 299 lipid kinase [Escherichia coli]
GI:16130026 gnl IGS 299 phosphatidylglycerol kinase, metal-dependent [Escherichia coli ER2796]

Related Sequences to LMP007552 proteins

Reference Database Accession Length Protein Name
GI:16130026 GenBank KDT19578.1 299 lipid kinase YegS [Escherichia coli 2-052-05_S3_C1]
GI:16130026 GenBank KDT19989.1 299 lipid kinase YegS [Escherichia coli 2-011-08_S4_C3]
GI:16130026 GenBank KDT79566.1 299 lipid kinase YegS [Escherichia coli 3-373-03_S1_C2]
GI:16130026 GenBank KDT91860.1 299 lipid kinase YegS [Escherichia coli 3-475-03_S1_C1]
GI:16130026 PDB 2BON 332 Chain A, Structure Of An Escherichia Coli Lipid Kinase (Yegs)
GI:16130026 PDB 2BON 332 Chain B, Structure Of An Escherichia Coli Lipid Kinase (Yegs)