Gene/Proteome Database (LMPD)

LMPD ID
LMP007565
Gene ID
945409
Species
Escherichia coli K-12 (E. coli)
Gene Name
cardiolipin synthase 2
Gene Symbol
clsB
Synonyms
ECK0778; JW0772; ybhO
Summary
None
Orthologs
View orthologs and multiple alignments for clsB

Proteins

cardiolipin synthase 2 [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_415310
Protein GI 16128757
UniProt ID P0AA84
Length 413
RefSeq Status REVIEWED
MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFEDDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLFGMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDILQFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVKGGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRDNLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDPPAQPTMETQDRVETENTGVKP

Gene Information

Entrez Gene ID
945409
Gene Name
cardiolipin synthase 2
Gene Symbol
clsB
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016020 IDA:EcoCyc C membrane
GO:0005886 IEA:UniProtKB-KW C plasma membrane
GO:0008808 IDA:EcoCyc F cardiolipin synthase activity
GO:0004630 IDA:EcoliWiki F phospholipase D activity
GO:0032049 IMP:EcoCyc P cardiolipin biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco00564 Glycerophospholipid metabolism
ko00564 Glycerophospholipid metabolism
eco01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-5668 cardiolipin biosynthesis I
PWY-5668 cardiolipin biosynthesis I
PHOSLIPSYN-PWY phospholipid biosynthesis I
PHOSLIPSYN-PWY phospholipid biosynthesis I

Domain Information

InterPro Annotations

Accession Description
IPR025202 Phospholipase D-like domain
IPR001736 Phospholipase D/Transphosphatidylase

UniProt Annotations

Entry Information

Gene Name
cardiolipin synthase 2
Protein Entry
CLSB_ECOLI
UniProt ID
P0AA84
Species
E. coli

Comments

Comment Type Description
Catalytic Activity 2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol. {ECO:0000255|HAMAP- Rule:MF_01917}.
Disruption Phenotype Triple deletion of clsA, clsB and clsC results in a complete lack of cardiolipin, regardless of growth phase or growth conditions. {ECO:0000269|PubMed:22988102}.
Enzyme Regulation Activated by phosphate. Inhibited by cardiolipin and phosphatidate. {ECO:0000269|PubMed:10634942}.
Function Catalyzes the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol. Can also catalyze phosphatidyl group transfer to water to form phosphatidate. {ECO:0000255|HAMAP-Rule:MF_01917, ECO:0000269|PubMed:10634942, ECO:0000269|PubMed:22988102}.
Miscellaneous All three cardiolipin synthases (ClsA, ClsB and ClsC) contribute to CL synthesis in stationary phase. Only ClsA contributes to synthesis during logarithmic growth phase (PubMed:22988102). {ECO:0000305|PubMed:22988102}.
Similarity Belongs to the phospholipase D family. Cardiolipin synthase subfamily. ClsB sub-subfamily. {ECO:0000255|HAMAP- Rule:MF_01917}.
Similarity Contains 2 PLD phosphodiesterase domains. {ECO:0000255|HAMAP-Rule:MF_01917}.
Subcellular Location Cell membrane {ECO:0000305|PubMed:10634942}; Peripheral membrane protein {ECO:0000305|PubMed:10634942}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007565 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16128757 RefSeq NP_415310 413 cardiolipin synthase 2 [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007565 proteins

Reference Database Accession Length Protein Name
GI:16128757 GenBank KHI99401.1 413 cardiolipin synthase 2 [Escherichia coli]
GI:16128757 GenBank KHJ10400.1 413 cardiolipin synthase 2 [Escherichia coli]
GI:16128757 GenBank KHJ14480.1 413 cardiolipin synthase 2 [Escherichia coli]
GI:16128757 GenBank KHJ21551.1 413 cardiolipin synthase 2 [Escherichia coli]
GI:16128757 GenBank KHJ25609.1 413 cardiolipin synthase 2 [Escherichia coli]
GI:16128757 gnl IGS 413 cardiolipin synthase 2 [Escherichia coli ER2796]

Related Sequences to LMP007565 proteins

Reference Database Accession Length Protein Name
GI:16128757 GenBank EOV14634.1 413 cardiolipin synthase 2 [Escherichia coli KTE40]
GI:16128757 GenBank EOW99200.1 413 cardiolipin synthase 2 [Escherichia coli KTE41]
GI:16128757 GenBank KDV38861.1 413 cardiolipin synthase 2 [Escherichia coli O146:H21 str. 2010C-3325]
GI:16128757 RefSeq YP_006167824.1 413 Putative cardiolipin synthetase ybhO [Escherichia coli P12b]
GI:16128757 RefSeq WP_000650369.1 413 cardiolipin synthase 2 [Escherichia coli]
GI:16128757 RefSeq WP_033882746.1 413 cardiolipin synthase 2 [Escherichia coli]