Gene/Proteome Database (LMPD)

LMPD ID
LMP007589
Gene ID
946327
Species
Escherichia coli K-12 (E. coli)
Gene Name
acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase)
Gene Symbol
fadD
Synonyms
ECK1803; JW1794; oldD
Summary
The FadD protein, encoding fatty acyl-CoA synthetase, can transport long chain fatty acids across the inner membrane in an in vitro system . [More information is available at EcoCyc: EG11530].
Orthologs
View orthologs and multiple alignments for fadD

Proteins

acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase) [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_416319
Protein GI 16129759
UniProt ID P69451
Length 561
RefSeq Status REVIEWED
MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVNMGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAKGTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELGGQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLVDDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSLTEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA

Gene Information

Entrez Gene ID
946327
Gene Name
acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase)
Gene Symbol
fadD
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:EcoliWiki C cytoplasm
GO:0009898 TAS:EcoCyc C cytoplasmic side of plasma membrane
GO:0005829 TAS:EcoCyc C cytosol
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0005504 IMP:EcoliWiki F fatty acid binding
GO:0004467 IDA:EcoCyc F long-chain fatty acid-CoA ligase activity
GO:0070538 IMP:EcoliWiki F oleic acid binding
GO:0006637 IDA:EcoCyc P acyl-CoA metabolic process
GO:0006635 IDA:EcoCyc P fatty acid beta-oxidation
GO:0006631 IMP:EcoliWiki P fatty acid metabolic process
GO:0006629 IMP:EcoliWiki P lipid metabolic process
GO:0001676 IDA:GOC P long-chain fatty acid metabolic process
GO:0008654 IMP:EcoliWiki P phospholipid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco_M00086 beta-Oxidation, acyl-CoA synthesis
M00086 beta-Oxidation, acyl-CoA synthesis
eco00061 Fatty acid biosynthesis
ko00061 Fatty acid biosynthesis
eco00071 Fatty acid degradation
ko00071 Fatty acid degradation
eco01212 Fatty acid metabolism
ko01212 Fatty acid metabolism
eco01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
FAO-PWY fatty acid beta-oxidation I
FAO-PWY fatty acid beta-oxidation I
FAO-PWY fatty acid beta-oxidation I
PWY-5136 fatty acid beta-oxidation II (core pathway)
PWY-561 superpathway of glyoxylate cycle

Domain Information

InterPro Annotations

Accession Description
IPR020845 AMP-binding, conserved site
IPR025110 AMP-binding enzyme C-terminal domain
IPR000873 AMP-dependent synthetase/ligase

UniProt Annotations

Entry Information

Gene Name
acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase)
Protein Entry
LCFA_ECOLI
UniProt ID
P69451
Species
E. coli

Comments

Comment Type Description
Catalytic Activity ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
Function Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids.
Miscellaneous Activity is the highest with fatty acid substrates of > 10 carbon atoms.
Similarity Belongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}.
Subcellular Location Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Partially membrane-associated. {ECO:0000250}.
Subunit Homodimer. {ECO:0000305}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007589 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16129759 RefSeq NP_416319 561 acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase) [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007589 proteins

Reference Database Accession Length Protein Name
GI:16129759 EMBL CDY58758.1 561 fatty acyl-CoA synthetase [Escherichia coli]
GI:16129759 EMBL CDZ20640.1 561 fatty acyl-CoA synthetase [Escherichia coli]
GI:16129759 GenBank KFB94217.1 561 long-chain-fatty-acid--CoA ligase [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
GI:16129759 GenBank KGL69316.1 561 long-chain-fatty-acid--CoA ligase [Escherichia coli NCTC 50110]
GI:16129759 gnl PRJNA257976 561 acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase) [Escherichia coli BW25113]
GI:16129759 gnl IGS 561 acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase) [Escherichia coli ER2796]

Related Sequences to LMP007589 proteins

Reference Database Accession Length Protein Name
GI:16129759 GenBank EQN03996.1 583 long-chain-fatty-acid-CoA ligase [Escherichia coli HVH 2 (4-6943160)]
GI:16129759 GenBank EQN19519.1 583 long-chain-fatty-acid-CoA ligase [Escherichia coli HVH 4 (4-7276109)]
GI:16129759 GenBank EQN34852.1 583 long-chain-fatty-acid-CoA ligase [Escherichia coli HVH 7 (4-7315031)]
GI:16129759 GenBank ERF53966.1 583 long-chain-fatty-acid-CoA ligase [Escherichia coli UMEA 3652-1]
GI:16129759 GenBank EYB63506.1 583 long-chain fatty acid--CoA ligase [Escherichia coli]
GI:16129759 GenBank KDW04846.1 561 long-chain-fatty-acid--CoA ligase [Escherichia coli 2-156-04_S1_C3]