Gene/Proteome Database (LMPD)

LMPD ID
LMP007595
Gene ID
945334
Species
Escherichia coli K-12 (E. coli)
Gene Name
undecaprenyl phosphate-alpha-L-ara4FN deformylase
Gene Symbol
arnD
Synonyms
ECK2249; JW2250; pbgP4; pmrJ; yfbH
Summary
An arnD mutant strain accumulates formylated C55-P-LAra4N and blocks C55-P-LAra4N production, supporting the deformaylase functional assignment (S.D. [More information is available at EcoGene: EG14092]. ArnD is predicted to act as a deformylase that removes the formyl group from undecaprenyl phosphate-L-Ara4FN . [More information is available at EcoCyc: G7169].
Orthologs
View orthologs and multiple alignments for arnD

Proteins

undecaprenyl phosphate-alpha-L-ara4FN deformylase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_416759
Protein GI 16130191
UniProt ID P76472
Length 296
RefSeq Status REVIEWED
MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDNMGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHEVGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIEAKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLLNRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVVRGNIAGREGWLGCQQIAGSR

Gene Information

Entrez Gene ID
945334
Gene Name
undecaprenyl phosphate-alpha-L-ara4FN deformylase
Gene Symbol
arnD
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016811 IEA:UniProtKB-HAMAP F hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
GO:0036108 IEA:UniProtKB-UniPathway P 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate biosynthetic process
GO:0009245 IEA:UniProtKB-HAMAP P lipid A biosynthetic process
GO:0009103 IEA:UniProtKB-UniPathway P lipopolysaccharide biosynthetic process
GO:0046677 IEA:UniProtKB-KW P response to antibiotic
GO:0010041 IGI:EcoCyc P response to iron(III) ion

Domain Information

InterPro Annotations

Accession Description
IPR023557 ArnD
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR002509 Polysac_deacetylase

UniProt Annotations

Entry Information

Gene Name
undecaprenyl phosphate-alpha-L-ara4FN deformylase
Protein Entry
ARND_ECOLI
UniProt ID
P76472
Species
E. coli

Comments

Comment Type Description
Catalytic Activity 4-deoxy-4-formamido-beta-L-arabinose di- trans,poly-cis-undecaprenyl phosphate + H(2)O = 4-amino-4-deoxy- alpha-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + formate. {ECO:0000255|HAMAP-Rule:MF_01870, ECO:0000269|PubMed:15695810}.
Function Catalyzes the deformylation of 4-deoxy-4-formamido-L- arabinose-phosphoundecaprenol to 4-amino-4-deoxy-L-arabinose- phosphoundecaprenol. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (Probable). {ECO:0000305|PubMed:15695810}.
Induction Induced by BasR. {ECO:0000250}.
Interaction P0A6F5:groL; NbExp=1; IntAct=EBI-1128209, EBI-543750;
Pathway Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01870, ECO:0000269|PubMed:17928292}.
Pathway Glycolipid biosynthesis; 4-amino-4-deoxy-alpha-L- arabinose undecaprenyl phosphate biosynthesis; 4-amino-4-deoxy- alpha-L-arabinose undecaprenyl phosphate from UDP-4-deoxy-4- formamido-beta-L-arabinose and undecaprenyl phosphate: step 2/2. {ECO:0000255|HAMAP-Rule:MF_01870, ECO:0000269|PubMed:17928292}.
Similarity Belongs to the polysaccharide deacetylase family. ArnD deformylase subfamily. {ECO:0000255|HAMAP-Rule:MF_01870}.
Similarity Contains 1 NodB homology domain. {ECO:0000255|HAMAP- Rule:MF_01870}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007595 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16130191 RefSeq NP_416759 296 undecaprenyl phosphate-alpha-L-ara4FN deformylase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007595 proteins

Reference Database Accession Length Protein Name
GI:16130191 GenBank KHJ03315.1 296 4-deoxy-4-formamido-L-arabinose-phospho-UDP deformylase [Escherichia coli]
GI:16130191 GenBank KHJ04100.1 296 4-deoxy-4-formamido-L-arabinose-phospho-UDP deformylase [Escherichia coli]
GI:16130191 GenBank KHJ10028.1 296 4-deoxy-4-formamido-L-arabinose-phospho-UDP deformylase [Escherichia coli]
GI:16130191 GenBank KHJ18201.1 296 4-deoxy-4-formamido-L-arabinose-phospho-UDP deformylase [Escherichia coli]
GI:16130191 GenBank KHJ24269.1 296 4-deoxy-4-formamido-L-arabinose-phospho-UDP deformylase [Escherichia coli]
GI:16130191 gnl IGS 296 undecaprenyl phosphate-alpha-L-ara4FN deformylase [Escherichia coli ER2796]

Related Sequences to LMP007595 proteins

Reference Database Accession Length Protein Name
GI:16130191 GenBank EJZ65277.1 296 hypothetical protein SF148580_2679 [Shigella flexneri 1485-80]
GI:16130191 GenBank ELF11754.1 296 undecaprenyl phosphate-alpha-L-ara4FN deformylase [Escherichia coli KTE142]
GI:16130191 RefSeq WP_001570110.1 296 undecaprenyl phosphate-alpha-L-ara4FN deformylase [Escherichia coli]
GI:16130191 RefSeq WP_024244011.1 296 4-deoxy-4-formamido-L-arabinose-phospho-UDP deformylase [Escherichia coli]
GI:16130191 SwissProt Q31YK1.1 296 RecName: Full=Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD [Shigella boydii Sb227]
GI:16130191 SwissProt B2TW39.1 296 RecName: Full=Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD [Shigella boydii CDC 3083-94]