Gene/Proteome Database (LMPD)
LMPD ID
LMP007606
Gene ID
Species
Escherichia coli K-12 (E. coli)
Gene Name
lipoate synthase
Gene Symbol
Synonyms
ECK0621; JW0623; lip
Summary
Radical SAM superfamily. [More information is available at EcoGene: EG11306]. LipA is required for the step of lipoate biosynthesis at which sulfur is inserted into the octanoyl side-chain of an octanoylated E2 domain, to form the lipoate moiety . [More information is available at EcoCyc: EG11306].
Orthologs
Proteins
| lipoate synthase [Escherichia coli str. K-12 substr. MG1655] | |
|---|---|
| Refseq ID | NP_415161 |
| Protein GI | 16128611 |
| UniProt ID | P60716 |
| Length | 321 |
| RefSeq Status | REVIEWED |
| MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIKLPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKSPQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IDA:EcoliWiki | C | cytoplasm |
| GO:0051539 | IDA:EcoliWiki | F | 4 iron, 4 sulfur cluster binding |
| GO:0003826 | IMP:CACAO | F | alpha-ketoacid dehydrogenase activity |
| GO:0016992 | IDA:EcoliWiki | F | lipoate synthase activity |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0009107 | IMP:EcoliWiki | P | lipoate biosynthetic process |
| GO:0055114 | IMP:GOC | P | oxidation-reduction process |
| GO:0009249 | IDA:EcoliWiki | P | protein lipoylation |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| eco00785 | Lipoic acid metabolism |
| ko00785 | Lipoic acid metabolism |
| eco01100 | Metabolic pathways |
BIOCYC Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Protein N(6)-(octanoyl)lysine + 2 sulfur- (sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)- (lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'- deoxyadenosine. {ECO:0000269|PubMed:15157071}. |
| Cofactor | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000269|PubMed:15362861}; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L- methionine. {ECO:0000269|PubMed:15362861}; |
| Enzyme Regulation | Inhibited by the AdoMet analog S-adenosyl homocysteine. |
| Function | Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. Free octanoate is not a substrate for LipA. {ECO:0000269|PubMed:11106496, ECO:0000269|PubMed:14700636, ECO:0000269|PubMed:15157071}. |
| Miscellaneous | The source of sulfurs is the LipA protein itself, most likely one 4Fe-4S cluster. |
| Pathway | Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2. {ECO:0000269|PubMed:15112987}. |
| Sequence Caution | Sequence=AAA24072.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
| Similarity | Belongs to the radical SAM superfamily. Lipoyl synthase family. {ECO:0000305}. |
| Subcellular Location | Cytoplasm. |
| Subunit | Monomer or homodimer. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007606 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 16128611 | RefSeq | NP_415161 | 321 | lipoate synthase [Escherichia coli str. K-12 substr. MG1655] |
Identical Sequences to LMP007606 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16128611 | GenBank | KHJ07799.1 | 321 | lipoyl synthase [Escherichia coli] |
| GI:16128611 | GenBank | KHJ11079.1 | 321 | lipoyl synthase [Escherichia coli] |
| GI:16128611 | GenBank | KHJ16921.1 | 321 | lipoyl synthase [Escherichia coli] |
| GI:16128611 | GenBank | KHJ23072.1 | 321 | lipoyl synthase [Escherichia coli] |
| GI:16128611 | GenBank | KHJ28879.1 | 321 | lipoyl synthase [Escherichia coli] |
| GI:16128611 | gnl | IGS | 321 | lipoate synthase [Escherichia coli ER2796] |
Related Sequences to LMP007606 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16128611 | GenBank | AAA66345.1 | 321 | LIPA protein [Escherichia coli str. K-12 substr. W3110] |
| GI:16128611 | GenBank | EFZ76530.1 | 321 | lipoic acid synthetase [Escherichia coli RN587/1] |
| GI:16128611 | GenBank | EQR04751.1 | 321 | lipoyl synthase [Escherichia coli HVH 115 (4-4465989)] |
| GI:16128611 | GenBank | EQR09633.1 | 321 | lipoyl synthase [Escherichia coli HVH 115 (4-4465997)] |
| GI:16128611 | RefSeq | WP_000042626.1 | 321 | lipoyl synthase [Escherichia coli] |
| GI:16128611 | RefSeq | WP_021529496.1 | 321 | lipoyl synthase [Escherichia coli] |