Gene/Proteome Database (LMPD)

LMPD ID
LMP007662
Gene ID
945821
Species
Escherichia coli K-12 (E. coli)
Gene Name
cardiolipin synthase 1
Gene Symbol
clsA
Synonyms
ECK1243; JW1241; cls; nov; yciJ
Summary
Overexpression causes filamentous biofilm formation. [More information is available at EcoGene: EG11608].
Orthologs
View orthologs and multiple alignments for clsA

Proteins

cardiolipin synthase 1 [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_415765
Protein GI 16129210
UniProt ID P0A6H8
Length 486
RefSeq Status REVIEWED
MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAPLFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQVAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIYSCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAREYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAGVKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQDDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL

Gene Information

Entrez Gene ID
945821
Gene Name
cardiolipin synthase 1
Gene Symbol
clsA
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:EcoCyc C membrane
GO:0005886 IEA:UniProtKB-HAMAP C plasma membrane
GO:0008808 IDA:EcoCyc F cardiolipin synthase activity
GO:0032049 IMP:EcoCyc P cardiolipin biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco00564 Glycerophospholipid metabolism
ko00564 Glycerophospholipid metabolism
eco01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-5668 cardiolipin biosynthesis I
PWY-5668 cardiolipin biosynthesis I
PWY-5668 cardiolipin biosynthesis I
PHOSLIPSYN-PWY phospholipid biosynthesis I
PHOSLIPSYN-PWY phospholipid biosynthesis I
PHOSLIPSYN-PWY phospholipid biosynthesis I

Domain Information

InterPro Annotations

Accession Description
IPR027379 CLS_N
IPR022924 Cardiolipin_synthase
IPR001736 Phospholipase D/Transphosphatidylase

UniProt Annotations

Entry Information

Gene Name
cardiolipin synthase 1
Protein Entry
CLSA_ECOLI
UniProt ID
P0A6H8
Species
E. coli

Comments

Comment Type Description
Biophysicochemical Properties pH dependence: Optimum pH is 7.0. {ECO:0000269|PubMed:1663113};
Catalytic Activity 2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol. {ECO:0000255|HAMAP- Rule:MF_00190, ECO:0000269|PubMed:1663113, ECO:0000269|PubMed:19341704}.
Disruption Phenotype Mutants have increased sensitivity to novobiocin. Triple deletion of clsA, clsB and clsC results in a complete lack of cardiolipin, regardless of growth phase or growth conditions. {ECO:0000269|PubMed:19341704, ECO:0000269|PubMed:7665497}.
Function Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol. {ECO:0000255|HAMAP-Rule:MF_00190, ECO:0000269|PubMed:1663113, ECO:0000269|PubMed:19341704}.
Miscellaneous All three cardiolipin synthases (ClsA, ClsB and ClsC) contribute to CL synthesis in stationary phase. Only ClsA contributes to synthesis during logarithmic growth phase (PubMed:22988102). {ECO:0000305|PubMed:22988102}.
Ptm Seems to be post-translationally processed from a 53-55 kDa form to a 45-46 kDa form (PubMed:7665497 and PubMed:19341704).
Similarity Belongs to the phospholipase D family. Cardiolipin synthase subfamily. ClsA sub-subfamily. {ECO:0000255|HAMAP- Rule:MF_00190}.
Similarity Contains 2 PLD phosphodiesterase domains. {ECO:0000255|HAMAP-Rule:MF_00190}.
Subcellular Location Cell inner membrane {ECO:0000255|HAMAP- Rule:MF_00190, ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:1663113, ECO:0000269|PubMed:22988102}; Multi- pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00190, ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:1663113, ECO:0000269|PubMed:22988102}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007662 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16129210 RefSeq NP_415765 486 cardiolipin synthase 1 [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007662 proteins

Reference Database Accession Length Protein Name
GI:16129210 GenBank KHJ02877.1 486 cardiolipin synthetase [Escherichia coli]
GI:16129210 GenBank KHJ06688.1 486 cardiolipin synthetase [Escherichia coli]
GI:16129210 GenBank KHJ13572.1 486 cardiolipin synthetase [Escherichia coli]
GI:16129210 GenBank KHJ23534.1 486 cardiolipin synthetase [Escherichia coli]
GI:16129210 GenBank KHJ26294.1 486 cardiolipin synthetase [Escherichia coli]
GI:16129210 gnl IGS 486 cardiolipin synthase 1 [Escherichia coli ER2796]

Related Sequences to LMP007662 proteins

Reference Database Accession Length Protein Name
GI:16129210 GenBank EFX27078.1 486 cardiolipin synthetase [Escherichia coli O55:H7 str. USDA 5905]
GI:16129210 GenBank EKH96031.1 486 cardiolipin synthase [Escherichia coli 5905]
GI:16129210 gnl nkteda 486 Cardiolipin synthetase [Escherichia coli O55:H7 str. CB9615]
GI:16129210 gnl REF_nkteda 486 Cardiolipin synthetase [Escherichia coli O55:H7 str. CB9615]
GI:16129210 gnl USDA-ARS-WRRC-PSM 486 cardiolipin synthetase [Escherichia coli O55:H7 str. RM12579]
GI:16129210 RefSeq WP_000214518.1 486 cardiolipin synthetase [Escherichia coli]