Gene/Proteome Database (LMPD)
LMPD ID
LMP007693
Gene ID
Species
Escherichia coli K-12 (E. coli)
Gene Name
tetraacyldisaccharide-1-P synthase
Gene Symbol
Synonyms
ECK0181; JW0177; pgsB
Summary
The lpxB1 mutation is E326K, the result of a change in codon 326 from GAA to AAA (C.R.H. [More information is available at EcoGene: EG10546]. Lipid A disaccharide synthase (LpxB) catalyzes the fifth step in lipid A biosynthesis. [More information is available at EcoCyc: EG10546].
Orthologs
Proteins
| tetraacyldisaccharide-1-P synthase [Escherichia coli str. K-12 substr. MG1655] | |
|---|---|
| Refseq ID | NP_414724 |
| Protein GI | 16128175 |
| UniProt ID | P10441 |
| Length | 382 |
| RefSeq Status | REVIEWED |
| MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGPRMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDFNITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVPCRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQLLRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASGTAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEPQKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ | |
Gene Information
Entrez Gene ID
Gene Name
tetraacyldisaccharide-1-P synthase
Gene Symbol
Species
Escherichia coli K-12
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IDA:EcoCyc | C | cytoplasm |
| GO:0005829 | IDA:EcoCyc | C | cytosol |
| GO:0019897 | IDA:EcoCyc | C | extrinsic component of plasma membrane |
| GO:0008915 | IDA:EcoliWiki | F | lipid-A-disaccharide synthase activity |
| GO:0005543 | IDA:EcoliWiki | F | phospholipid binding |
| GO:0009245 | IMP:EcoliWiki | P | lipid A biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| eco00540 | Lipopolysaccharide biosynthesis |
| ko00540 | Lipopolysaccharide biosynthesis |
| M00060 | Lipopolysaccharide biosynthesis, KDO2-lipid A |
| eco_M00060 | Lipopolysaccharide biosynthesis, KDO2-lipid A |
| eco01100 | Metabolic pathways |
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| NAGLIPASYN-PWY | lipid IVA biosynthesis |
| NAGLIPASYN-PWY | lipid IVA biosynthesis |
| KDO-NAGLIPASYN-PWY | superpathway of (KDO)2-lipid A biosynthesis |
| KDO-NAGLIPASYN-PWY | superpathway of (KDO)2-lipid A biosynthesis |
| LPSSYN-PWY | superpathway of lipopolysaccharide biosynthesis |
| LPSSYN-PWY | superpathway of lipopolysaccharide biosynthesis |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR003835 | Glycosyl transferase, family 19 |
UniProt Annotations
Entry Information
Gene Name
tetraacyldisaccharide-1-P synthase
Protein Entry
LPXB_ECOLI
UniProt ID
Species
E. coli
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D- 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. |
| Function | Condensation of UDP-2,3-diacylglucosamine and 2,3- diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. |
| Interaction | P0CE47:tufA; NbExp=2; IntAct=EBI-553692, EBI-301077; |
| Pathway | Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 5/6. |
| Similarity | Belongs to the LpxB family. {ECO:0000305}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007693 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 16128175 | RefSeq | NP_414724 | 382 | tetraacyldisaccharide-1-P synthase [Escherichia coli str. K-12 substr. MG1655] |
Identical Sequences to LMP007693 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16128175 | GenBank | KHI14800.1 | 382 | lipid-A-disaccharide synthase [Escherichia coli] |
| GI:16128175 | GenBank | KHI20637.1 | 382 | lipid-A-disaccharide synthase [Escherichia coli] |
| GI:16128175 | GenBank | KHI57995.1 | 382 | lipid-A-disaccharide synthase [Escherichia coli] |
| GI:16128175 | GenBank | KHI63512.1 | 382 | lipid-A-disaccharide synthase [Escherichia coli] |
| GI:16128175 | GenBank | KHJ15471.1 | 382 | lipid-A-disaccharide synthase [Escherichia coli] |
| GI:16128175 | gnl | IGS | 382 | tetraacyldisaccharide-1-P synthase [Escherichia coli ER2796] |
Related Sequences to LMP007693 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16128175 | EMBL | CCQ03794.1 | 382 | Lipid-A-disaccharide synthase [Escherichia coli O5:K4(L):H4 str. ATCC 23502] |
| GI:16128175 | GenBank | ELC64652.1 | 382 | lipid-A-disaccharide synthase [Escherichia coli KTE44] |
| GI:16128175 | GenBank | EQX00289.1 | 382 | lipid-A-disaccharide synthase [Escherichia coli UMEA 3139-1] |
| GI:16128175 | GenBank | ERB35237.1 | 382 | lipid-A-disaccharide synthase [Escherichia coli UMEA 3271-1] |
| GI:16128175 | GenBank | KDX87996.1 | 382 | lipid-A-disaccharide synthase [Escherichia coli 2-222-05_S4_C2] |
| GI:16128175 | RefSeq | WP_001529688.1 | 382 | lipid-A-disaccharide synthase [Escherichia coli] |