Gene/Proteome Database (LMPD)
LMPD ID
LMP007712
Gene ID
Species
Escherichia coli K-12 (E. coli)
Gene Name
beta-ketoacyl-CoA thiolase, anaerobic, subunit
Gene Symbol
Synonyms
ECK2336; JW2339; yfcY
Summary
Also known as beta-oxidation complex II. [More information is available at EcoGene: EG14128]. During anaerobic beta-oxidation of fatty acids FadI, FadJ, and FadK serve functions parallel to those of FadA, FadB, and FadD in the aerobic pathway . [More information is available at EcoCyc: G7213].
Orthologs
Proteins
| beta-ketoacyl-CoA thiolase, anaerobic, subunit [Escherichia coli str. K-12 substr. MG1655] | |
|---|---|
| Refseq ID | NP_416844 |
| Protein GI | 16130275 |
| UniProt ID | P76503 |
| Length | 436 |
| RefSeq Status | REVIEWED |
| MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE | |
Gene Information
Entrez Gene ID
Gene Name
beta-ketoacyl-CoA thiolase, anaerobic, subunit
Gene Symbol
Species
Escherichia coli K-12
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IDA:EcoliWiki | C | cytoplasm |
| GO:0003857 | IDA:EcoliWiki | F | 3-hydroxyacyl-CoA dehydrogenase activity |
| GO:0003988 | IDA:EcoliWiki | F | acetyl-CoA C-acyltransferase activity |
| GO:0004300 | IDA:EcoliWiki | F | enoyl-CoA hydratase activity |
| GO:0033542 | IMP:EcoliWiki | P | fatty acid beta-oxidation, unsaturated, even number |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| eco00592 | alpha-Linolenic acid metabolism |
| ko00592 | alpha-Linolenic acid metabolism |
| eco00362 | Benzoate degradation |
| ko00362 | Benzoate degradation |
| eco_M00087 | beta-Oxidation |
| M00087 | beta-Oxidation |
| eco01110 | Biosynthesis of secondary metabolites |
| eco00071 | Fatty acid degradation |
| ko00071 | Fatty acid degradation |
| eco01212 | Fatty acid metabolism |
| ko01212 | Fatty acid metabolism |
| eco00281 | Geraniol degradation |
| ko00281 | Geraniol degradation |
| eco01100 | Metabolic pathways |
| eco01120 | Microbial metabolism in diverse environments |
| eco00280 | Valine, leucine and isoleucine degradation |
| ko00280 | Valine, leucine and isoleucine degradation |
BIOCYC Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR012806 | Ac-CoA_C-AcTrfase_FadI |
| IPR002155 | Thiolase |
| IPR020610 | Thiolase, active site |
| IPR020615 | Thiolase, acyl-enzyme intermediate active site |
| IPR020613 | Thiolase, conserved site |
| IPR020617 | Thiolase, C-terminal |
| IPR016039 | Thiolase-like |
| IPR016038 | Thiolase-like, subgroup |
| IPR020616 | Thiolase, N-terminal |
UniProt Annotations
Entry Information
Gene Name
beta-ketoacyl-CoA thiolase, anaerobic, subunit
Protein Entry
FADI_ECOLI
UniProt ID
Species
E. coli
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA. |
| Function | Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed. Strongly involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate and weakly involved in the aerobic degradation of long- chain fatty acids. {ECO:0000269|PubMed:12270828, ECO:0000269|PubMed:12535077}. |
| Induction | Unlike the aerobic pathway, the anaerobic pathway is not strongly repressed by FadR regulatory protein. |
| Pathway | Lipid metabolism; fatty acid beta-oxidation. |
| Similarity | Belongs to the thiolase family. {ECO:0000305}. |
| Subcellular Location | Cytoplasm {ECO:0000250}. |
| Subunit | Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI). {ECO:0000250}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007712 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 16130275 | RefSeq | NP_416844 | 436 | beta-ketoacyl-CoA thiolase, anaerobic, subunit [Escherichia coli str. K-12 substr. MG1655] |
Identical Sequences to LMP007712 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16130275 | GenBank | KHH61846.1 | 436 | 3-ketoacyl-CoA thiolase [Escherichia coli] |
| GI:16130275 | GenBank | KHH68447.1 | 436 | 3-ketoacyl-CoA thiolase [Escherichia coli] |
| GI:16130275 | GenBank | KHH89813.1 | 436 | 3-ketoacyl-CoA thiolase [Escherichia coli] |
| GI:16130275 | GenBank | KHI21515.1 | 436 | 3-ketoacyl-CoA thiolase [Escherichia coli] |
| GI:16130275 | GenBank | KHI60668.1 | 436 | 3-ketoacyl-CoA thiolase [Escherichia coli] |
| GI:16130275 | gnl | IGS | 436 | beta-ketoacyl-CoA thiolase, anaerobic, subunit [Escherichia coli ER2796] |
Related Sequences to LMP007712 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16130275 | GenBank | EYX09400.1 | 436 | 3-ketoacyl-CoA thiolase [Escherichia coli O69:H11 str. 07-4281] |
| GI:16130275 | GenBank | EYX75658.1 | 436 | 3-ketoacyl-CoA thiolase [Escherichia coli O111:NM str. 2011C-3679] |
| GI:16130275 | GenBank | EYX80857.1 | 436 | 3-ketoacyl-CoA thiolase [Escherichia coli O111:NM str. 2011C-3632] |
| GI:16130275 | GenBank | EYX91364.1 | 436 | 3-ketoacyl-CoA thiolase [Escherichia coli O111:NM str. 2011C-3573] |
| GI:16130275 | GenBank | KEN42706.1 | 436 | acetyl-CoA C-acyltransferase FadI [Escherichia coli 7-233-03_S4_C1] |
| GI:16130275 | RefSeq | WP_032295590.1 | 436 | 3-ketoacyl-CoA thiolase [Escherichia coli] |