Gene/Proteome Database (LMPD)
LMPD ID
LMP007719
Gene ID
Species
Escherichia coli K-12 (E. coli)
Gene Name
colanic biosynthesis UDP-glucose lipid carrier transferase
Gene Symbol
Synonyms
ECK2041; JW2032
Summary
Overexpression causes abnormal biofilm architecture. [More information is available at EcoGene: EG13575]. Inactivation of wcaJ results in an increase in the cellular pool of the colanic acid (CA) synthesis building block GDP-fucose, suggesting that WcaJ is involved in colanic acid synthesis . [More information is available at EcoCyc: G7098].
Orthologs
Proteins
| colanic biosynthesis UDP-glucose lipid carrier transferase [Escherichia coli str. K-12 substr. MG1655] | |
|---|---|
| Refseq ID | NP_416551 |
| Protein GI | 16129987 |
| UniProt ID | P71241 |
| Length | 464 |
| RefSeq Status | REVIEWED |
| MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYALTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLMESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGARVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRAEDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY | |
Gene Information
Entrez Gene ID
Gene Name
colanic biosynthesis UDP-glucose lipid carrier transferase
Gene Symbol
Species
Escherichia coli K-12
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0005886 | IEA:UniProtKB-KW | C | plasma membrane |
| GO:0016757 | IEA:UniProtKB-KW | F | transferase activity, transferring glycosyl groups |
| GO:0009242 | IMP:EcoCyc | P | colanic acid biosynthetic process |
| GO:0009103 | IEA:UniProtKB-KW | P | lipopolysaccharide biosynthetic process |
Domain Information
UniProt Annotations
Entry Information
Gene Name
colanic biosynthesis UDP-glucose lipid carrier transferase
Protein Entry
WCAJ_ECOLI
UniProt ID
Species
E. coli
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | UDP-glucose + ditrans,octacis-undecaprenyl phosphate = UMP + alpha-D-glucopyranosyl-diphospho- ditrans,octacis-undecaprenol. {ECO:0000269|PubMed:22408159}. |
| Disruption Phenotype | Cells lacking this gene show a non-mucoid phenotype. {ECO:0000269|PubMed:22408159}. |
| Function | Is the initiating enzyme for colanic acid (CA) synthesis. Catalyzes the transfer of the glucose-1-phosphate moiety from UDP-Glc onto the carrier lipid undecaprenyl phosphate (C55-P), forming a phosphoanhydride bond yielding to glucosyl- pyrophosphoryl-undecaprenol (Glc-PP-C55). Also possesses a weak galactose-1-P transferase activity. {ECO:0000269|PubMed:22408159}. |
| Miscellaneous | Complements holdfast synthesis in the C.crescentus mutant lacking hfsE, pssY and pssZ genes. Can also partially complement the O-antigen defect in the S.typhimurium wbaP deletion strain (PubMed:22408159). {ECO:0000305|PubMed:22408159}. |
| Pathway | Exopolysaccharide biosynthesis; colanic acid biosynthesis. {ECO:0000269|PubMed:22408159}. |
| Similarity | Belongs to the bacterial sugar transferase family. {ECO:0000305}. |
| Subcellular Location | Cell inner membrane {ECO:0000269|PubMed:22408159}; Multi-pass membrane protein {ECO:0000269|PubMed:22408159}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007719 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 16129987 | RefSeq | NP_416551 | 464 | colanic biosynthesis UDP-glucose lipid carrier transferase [Escherichia coli str. K-12 substr. MG1655] |
Identical Sequences to LMP007719 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16129987 | EMBL | CDY59353.1 | 464 | predicted colanic acid biosynthsis UDP-glucose lipid carrier transferase [Escherichia coli] |
| GI:16129987 | EMBL | CDZ20868.1 | 464 | predicted colanic acid biosynthsis UDP-glucose lipid carrier transferase [Escherichia coli] |
| GI:16129987 | GenBank | KGA85591.1 | 464 | UDP-glucose lipid carrier transferase [Escherichia coli] |
| GI:16129987 | GenBank | KGL68985.1 | 464 | UDP-glucose lipid carrier transferase [Escherichia coli NCTC 50110] |
| GI:16129987 | gnl | PRJNA257976 | 464 | colanic biosynthesis UDP-glucose lipid carrier transferase [Escherichia coli BW25113] |
| GI:16129987 | gnl | IGS | 464 | colanic biosynthesis UDP-glucose lipid carrier transferase [Escherichia coli ER2796] |
Related Sequences to LMP007719 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16129987 | GenBank | EZJ21765.1 | 464 | undecaprenyl-phosphate glucose phosphotransferase [Escherichia coli 1-182-04_S4_C3] |
| GI:16129987 | GenBank | EZJ23493.1 | 464 | undecaprenyl-phosphate glucose phosphotransferase [Escherichia coli 1-176-05_S4_C3] |
| GI:16129987 | GenBank | EZJ37969.1 | 464 | undecaprenyl-phosphate glucose phosphotransferase [Escherichia coli 1-250-04_S4_C2] |
| GI:16129987 | GenBank | EZJ42071.1 | 464 | undecaprenyl-phosphate glucose phosphotransferase [Escherichia coli 1-182-04_S4_C2] |
| GI:16129987 | GenBank | EZJ54729.1 | 464 | undecaprenyl-phosphate glucose phosphotransferase [Escherichia coli 1-250-04_S4_C1] |
| GI:16129987 | RefSeq | WP_025670468.1 | 464 | UDP-glucose lipid carrier transferase [Escherichia coli] |