Gene/Proteome Database (LMPD)

LMPD ID
LMP007730
Gene ID
946665
Species
Escherichia coli K-12 (E. coli)
Gene Name
3-oxoacyl-[acyl-carrier-protein] synthase II
Gene Symbol
fabF
Synonyms
ECK1081; JW1081; cvc; fabJ; vtr
Summary
There are three -ketoacyl-ACP synthases (KAS) in Escherichia coli: |FRAME: [More information is available at EcoCyc: EG12606].
Orthologs
View orthologs and multiple alignments for fabF

Proteins

3-oxoacyl-[acyl-carrier-protein] synthase II [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_415613
Protein GI 16129058
UniProt ID P0AAI5
Length 413
RefSeq Status REVIEWED
MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTSAYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIGAAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISIATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQAASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPENGAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSSTKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGMEYTLCNSFGFGGTNGSLIFKKI

Gene Information

Entrez Gene ID
946665
Gene Name
3-oxoacyl-[acyl-carrier-protein] synthase II
Gene Symbol
fabF
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:UniProtKB C cytosol
GO:0004315 IDA:EcoCyc F 3-oxoacyl-[acyl-carrier-protein] synthase activity
GO:0033817 IDA:CACAO F beta-ketoacyl-acyl-carrier-protein synthase II activity
GO:0006633 IEA:UniProtKB-UniPathway P fatty acid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco00780 Biotin metabolism
ko00780 Biotin metabolism
eco00061 Fatty acid biosynthesis
ko00061 Fatty acid biosynthesis
eco_M00083 Fatty acid biosynthesis, elongation
M00083 Fatty acid biosynthesis, elongation
eco01212 Fatty acid metabolism
ko01212 Fatty acid metabolism
eco01100 Metabolic pathways
eco_M00572 Pimeloyl-ACP biosynthesis, BioC-BioH pathway, malonyl-ACP => pimeloyl-ACP
M00572 Pimeloyl-ACP biosynthesis, BioC-BioH pathway, malonyl-ACP => pimeloyl-ACP

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6519 7-keto-8-aminopelargonate biosynthesis I
BIOTIN-BIOSYNTHESIS-PWY biotin biosynthesis I
PWY-5973 cis-vaccenate biosynthesis
PWY-5973 cis-vaccenate biosynthesis
PWY-5966 fatty acid biosynthesis initiation II
PWY-5966 fatty acid biosynthesis initiation II
PWY-5966 fatty acid biosynthesis initiation II
PWY-5965 fatty acid biosynthesis initiation III
PWY-5965 fatty acid biosynthesis initiation III
PWY-5965 fatty acid biosynthesis initiation III
FASYN-ELONG-PWY fatty acid elongation -- saturated
FASYN-ELONG-PWY fatty acid elongation -- saturated
FASYN-ELONG-PWY fatty acid elongation -- saturated
PWY0-881 superpathway of fatty acid biosynthesis I (E. coli)
PWY0-881 superpathway of fatty acid biosynthesis I (E. coli)
FASYN-INITIAL-PWY superpathway of fatty acid biosynthesis initiation (E. coli)
FASYN-INITIAL-PWY superpathway of fatty acid biosynthesis initiation (E. coli)
FASYN-INITIAL-PWY superpathway of fatty acid biosynthesis initiation (E. coli)
PWY-6285 superpathway of fatty acids biosynthesis (E. coli)
PWY-6284 superpathway of unsaturated fatty acids biosynthesis (E. coli)
PWY-6284 superpathway of unsaturated fatty acids biosynthesis (E. coli)

Domain Information

InterPro Annotations

Accession Description
IPR017568 3-oxoacyl-[acyl-carrier-protein] synthase 2
IPR018201 Beta-ketoacyl synthase, active site
IPR014031 Beta-ketoacyl synthase, C-terminal
IPR014030 Beta-ketoacyl synthase, N-terminal
IPR016039 Thiolase-like
IPR016038 Thiolase-like, subgroup

UniProt Annotations

Entry Information

Gene Name
3-oxoacyl-[acyl-carrier-protein] synthase II
Protein Entry
FABF_ECOLI
UniProt ID
P0AAI5
Species
E. coli

Comments

Comment Type Description
Catalytic Activity (Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl- carrier-protein] + CO(2) + [acyl-carrier-protein]. {ECO:0000269|PubMed:9013860}.
Function Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Has a preference for short chain acid substrates and may function to supply the octanoic substrates for lipoic acid biosynthesis. {ECO:0000269|PubMed:6988423, ECO:0000269|PubMed:9013860}.
Interaction P0A6Y8:dnaK; NbExp=2; IntAct=EBI-542783, EBI-542092;
Pathway Lipid metabolism; fatty acid biosynthesis.
Similarity Belongs to the beta-ketoacyl-ACP synthases family. {ECO:0000305}.
Subunit Homodimer.

Identical and Related Proteins

Unique RefSeq proteins for LMP007730 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16129058 RefSeq NP_415613 413 3-oxoacyl-[acyl-carrier-protein] synthase II [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007730 proteins

Reference Database Accession Length Protein Name
GI:16129058 GenBank KHI94543.1 413 3-oxoacyl-ACP synthase [Escherichia coli]
GI:16129058 GenBank KHJ05630.1 413 3-oxoacyl-ACP synthase [Escherichia coli]
GI:16129058 GenBank KHJ10637.1 413 3-oxoacyl-ACP synthase [Escherichia coli]
GI:16129058 GenBank KHJ17522.1 413 3-oxoacyl-ACP synthase [Escherichia coli]
GI:16129058 GenBank KHJ22766.1 413 3-oxoacyl-ACP synthase [Escherichia coli]
GI:16129058 gnl IGS 413 3-oxoacyl-[acyl-carrier-protein] synthase II [Escherichia coli ER2796]

Related Sequences to LMP007730 proteins

Reference Database Accession Length Protein Name
GI:16129058 GenBank ETY51408.1 413 3-oxoacyl-[acyl-carrier-protein] synthase 2 [Escherichia coli BIDMC 49b]
GI:16129058 GenBank ETY54128.1 413 3-oxoacyl-[acyl-carrier-protein] synthase 2 [Escherichia coli BIDMC 49a]
GI:16129058 GenBank EYY26402.1 413 3-oxoacyl-ACP synthase [Escherichia coli O121:H19 str. 2011C-3072]
GI:16129058 gnl mgcchina 413 3-oxoacyl-[acyl-carrier-protein] synthase II [Shigella sonnei Ss046]
GI:16129058 RefSeq WP_000044674.1 413 3-oxoacyl-ACP synthase [Shigella sonnei]
GI:16129058 RefSeq WP_032140064.1 413 3-oxoacyl-ACP synthase [Escherichia coli]