Gene/Proteome Database (LMPD)
Proteins
| Protein EGL-8, isoform a | |
|---|---|
| Refseq ID | NP_001021346 |
| Protein GI | 71981791 |
| UniProt ID | G5EBH0 |
| mRNA ID | NM_001026175 |
| Length | 1419 |
| RefSeq Status | REVIEWED |
| MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV | |
| Protein EGL-8, isoform b | |
|---|---|
| Refseq ID | NP_001021347 |
| Protein GI | 71981793 |
| UniProt ID | G5EBH0 |
| mRNA ID | NM_001026176 |
| Length | 1431 |
| RefSeq Status | REVIEWED |
| MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPRSGGSGGTGAPEEVDDDTSDDDDDPSVQTSLNVMRTIPTVNTTSNNGSNRSARSSLDTPSPSGGSLMVPDRATSTATSIKNAVLARSPNFSSLRQKLSFKRRQSPLAGDQRAHPEVEQPVSSSSPATPSISGPPPCATSSGSTSSITITTTGCSTSSSGPSKHILGGEMPAKENDEAHPELKQNFIAKNLKGFGFSKKQPVLTKEEEERIFAEYHYTGATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVDDSDIPDVPNTRNMALRHVKQPPRQNGSSADLLANNGQTGSARGDQTSSMASSTIRSPNEQPQPVAVDKFKVDPIEVDDLRRDKAFAKLLKRFQKELDDLRKKHQKQRDSIQKQQPARRRNSSIAWIQTNVDKLITNNRRSTKKEKGSRRSLTASVSSGCGSASGTVTVSVCSPSGASCSGYSTGGPSTPVACNSDGTGSPATIGSPVPQDLVNNDRVRSLVNTQTGEWSAMVRRHDEEEFELKKVQLKEQFDLLRKLMSEAQKNQMLALKLRLEAEGKDLKQTQTKKSMEDAKVIQLDKGIKTKAERDRRVKELNEKNLKMFVEERKRLAMKAQKHEEQLTKRHLDQLEQLDKDFHKALDAEVGNYKEEQLAAQPTSVV | |
| Protein EGL-8, isoform c | |
|---|---|
| Refseq ID | NP_001021348 |
| Protein GI | 71981799 |
| UniProt ID | G5EBH0 |
| mRNA ID | NM_001026177 |
| Length | 592 |
| RefSeq Status | REVIEWED |
| MAKEFQFNWKPTIIPELLHGSVFDRYDDESTCLELNAQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGGLPKDGRIMFELEQRGASETIAERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKERLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSPNRLRKKILIKNKRLKTDIERHQLDQFLREGKLDEEDELNETPEVVGEDSVSPREMPAKENDEAHPELKQNFIAKNLKGFGFSKKQETNKISNFNIVFKGFLFLLILILLSQIP | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0030054 | IEA:UniProtKB-KW | C | cell junction |
| GO:0042995 | IEA:UniProtKB-KW | C | cell projection |
| GO:0005886 | IDA:WormBase | C | plasma membrane |
| GO:0045202 | IEA:UniProtKB-KW | C | synapse |
| GO:0005509 | IEA:InterPro | F | calcium ion binding |
| GO:0004435 | IEA:UniProtKB-EC | F | phosphatidylinositol phospholipase C activity |
| GO:0004871 | IEA:UniProtKB-KW | F | signal transducer activity |
| GO:0006935 | IGI:WormBase | P | chemotaxis |
| GO:0007212 | IMP:WormBase | P | dopamine receptor signaling pathway |
| GO:0035556 | IEA:InterPro | P | intracellular signal transduction |
| GO:0016042 | IEA:UniProtKB-KW | P | lipid catabolic process |
| GO:0014057 | IMP:WormBase | P | positive regulation of acetylcholine secretion, neurotransmission |
| GO:0040017 | IMP:WormBase | P | positive regulation of locomotion |
| GO:0035418 | IGI:WormBase | P | protein localization to synapse |
| GO:0040012 | IGI:WormBase | P | regulation of locomotion |
| GO:0046662 | IMP:WormBase | P | regulation of oviposition |
| GO:0043051 | IMP:WormBase | P | regulation of pharyngeal pumping |
| GO:0043052 | IGI:WormBase | P | thermotaxis |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR000008 | C2 domain |
| IPR011992 | EF-hand domain pair |
| IPR009535 | Phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta, conserved site |
| IPR001192 | Phosphoinositide phospholipase C family |
| IPR000909 | Phospholipase C, phosphatidylinositol-specific , X domain |
| IPR001711 | Phospholipase C, phosphatidylinositol-specific, Y domain |
| IPR015359 | Phospholipase C, phosphoinositol-specific, EF-hand-like |
| IPR017946 | PLC-like phosphodiesterase, TIM beta/alpha-barrel domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=3; Name=b ; IsoId=G5EBH0-1; Sequence=Displayed; Name=a ; IsoId=G5EBH0-2; Sequence=VSP_053885; Name=c ; IsoId=G5EBH0-3; Sequence=VSP_053882, VSP_053883, VSP_053884; Note=No experimental confirmation available. ; |
| Catalytic Activity | 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. |
| Cofactor | Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence= ; |
| Disruption Phenotype | Mutant worms are similar in size to the wild-type but animals are bloated with eggs due to defective egg laying with decreased body flexion and sluggish locomotion. They have defects in the posterior body contraction step of the defecation motor program and become sluggish in the presence of exogenous betaine. Animals are resistant to aldicarb, a carbamate insecticide. Simultaneous knockout of egl-8 and snf-3 results in uncoordinated, hypercontracted and paralyzed animals. {ECO:0000269|PubMed:10571227, ECO:0000269|PubMed:10571228, ECO:0000269|PubMed:24212673}. |
| Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required in the nervous system to modulate neuronal activity. Facilitates synaptic transmission at neuromuscular junctions by regulating the release of acetylcholine from the motor neurons and thus affecting locomotion. Plays a role in efficient egg laying and defecation. {ECO:0000250|UniProtKB:P10687, ECO:0000269|PubMed:10571227, ECO:0000269|PubMed:10571228, ECO:0000269|PubMed:24212673}. |
| Similarity | Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}. |
| Similarity | Contains 1 PI-PLC X-box domain. {ECO:0000255|PROSITE- ProRule:PRU00270}. |
| Similarity | Contains 1 PI-PLC Y-box domain. {ECO:0000255|PROSITE- ProRule:PRU00271}. |
| Subcellular Location | Perikaryon . Cell projection, axon . Cell junction, synapse . Cell junction, adherens junction . Note=Present in neuronal cell somas and axon processes of the nerve ring in head ganglia. Present in the adherens junction of intestinal cells. |
| Tissue Specificity | Expressed in most or all neurons with high expression in the head and tail ganglia and low expression in the motor neurons of the ventral cord. Expressed in the intestine (at protein level). {ECO:0000269|PubMed:10571227, ECO:0000269|PubMed:10571228}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007876 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 71981791 | RefSeq | NP_001021346 | 1419 | Protein EGL-8, isoform a |
| 71981793 | RefSeq | NP_001021347 | 1431 | Protein EGL-8, isoform b |
| 71981799 | RefSeq | NP_001021348 | 592 | Protein EGL-8, isoform c |
Identical Sequences to LMP007876 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:71981793 | EMBL | CBU87825.1 | 1431 | unnamed protein product [Caenorhabditis elegans] |
| GI:71981793 | EMBL | CBU96772.1 | 1431 | unnamed protein product [Caenorhabditis elegans] |
| GI:71981791 | EMBL | CCD61782.1 | 1419 | Protein EGL-8, isoform a [Caenorhabditis elegans] |
| GI:71981793 | EMBL | CCD61783.1 | 1431 | Protein EGL-8, isoform b [Caenorhabditis elegans] |
| GI:71981799 | EMBL | CCD61784.1 | 592 | Protein EGL-8, isoform c [Caenorhabditis elegans] |
| GI:71981793 | GenBank | AGX54760.1 | 1431 | Sequence 9876 from patent US 8541208 |
| GI:71981793 | GenBank | AGX64200.1 | 1431 | Sequence 28510 from patent US 8541208 |
| GI:71981793 | SwissProt | G5EBH0.1 | 1431 | RecName: Full=1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8; AltName: Full=Egg-laying defective protein 8; AltName: Full=Phosphoinositide phospholipase C-beta egl-8; AltName: Full=Phospholipase C-beta egl-8; Short=PLC-beta egl-8 [Caenorhabditis elegans] |
Related Sequences to LMP007876 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:71981799 | EMBL | CBU87825.1 | 1431 | unnamed protein product [Caenorhabditis elegans] |
| GI:71981791 | EMBL | CBU87825.1 | 1431 | unnamed protein product [Caenorhabditis elegans] |
| GI:71981799 | EMBL | CBU96772.1 | 1431 | unnamed protein product [Caenorhabditis elegans] |
| GI:71981791 | EMBL | CBU96772.1 | 1431 | unnamed protein product [Caenorhabditis elegans] |
| GI:71981793 | EMBL | CCD61782.1 | 1419 | Protein EGL-8, isoform a [Caenorhabditis elegans] |
| GI:71981791 | EMBL | CCD61783.1 | 1431 | Protein EGL-8, isoform b [Caenorhabditis elegans] |
| GI:71981799 | EMBL | CCD61783.1 | 1431 | Protein EGL-8, isoform b [Caenorhabditis elegans] |
| GI:71981793 | GenBank | AAF01458.1 | 1418 | phospholipase C beta [Caenorhabditis elegans] |
| GI:71981791 | GenBank | AAF05701.1 | 1431 | phospholipase C beta homolog EGL-8 [Caenorhabditis elegans] |
| GI:71981799 | GenBank | AAF05701.1 | 1431 | phospholipase C beta homolog EGL-8 [Caenorhabditis elegans] |
| GI:71981793 | GenBank | EGT54056.1 | 1447 | hypothetical protein CAEBREN_25584 [Caenorhabditis brenneri] |
| GI:71981791 | GenBank | AGX54760.1 | 1431 | Sequence 9876 from patent US 8541208 |
| GI:71981799 | GenBank | AGX54760.1 | 1431 | Sequence 9876 from patent US 8541208 |
| GI:71981791 | GenBank | AGX64200.1 | 1431 | Sequence 28510 from patent US 8541208 |
| GI:71981799 | GenBank | AGX64200.1 | 1431 | Sequence 28510 from patent US 8541208 |
| GI:71981793 | RefSeq | NP_001021346.1 | 1419 | Protein EGL-8, isoform a [Caenorhabditis elegans] |
| GI:71981793 | RefSeq | XP_002647328.1 | 1431 | C. briggsae CBR-EGL-8 protein [Caenorhabditis briggsae] |
| GI:71981793 | RefSeq | XP_003115873.1 | 1437 | CRE-EGL-8 protein [Caenorhabditis remanei] |