Gene/Proteome Database (LMPD)

LMPD ID
LMP007889
Gene ID
180889
Species
Caenorhabditis elegans (C. elegans)
Gene Name
Protein DAF-9
Gene Symbol
daf-9
Synonyms
CELE_T13C5.1
Alternate Names
Protein DAF-9
Chromosome
X
EC Number
1.14.13.15

Proteins

Protein DAF-9, isoform a
Refseq ID NP_741807
Protein GI 71996277
UniProt ID H2KYS3
mRNA ID NM_171699
Length 572
RefSeq Status REVIEWED
MHLENRVLSSVLDYASKFYKRMSSLVFFSANSIQVQMNISQSSWIKCRDWMAFALSHHIIMGIYLLILRNFLPQVVPDFEWQHYFMRVFIVHIIYIIISYFIRITRYPPGPPPMAVFGNSPFVNILTPEQTFLEYREIYGPIFTLHLSQPTIILAEYKTIQEALVKNGQQTSGRSSAESFVLFTGDRLNGDGVILAMRQKWKDMRHEISRFMNKWYGAPMDELVLHHTRCLEQELAKIAETKSLIDLRDPLAGAIANVIQQITIGRNYMYQDQEFQTQLRDINAVVKEIMTAEVFFVNCYPWLRYLPEGILRKWTNYKRSGFRLQQWFRTILEEHHVNRHQGDFMSHMIDLQESKQEQFRDLSIILTCGDMWTGGMETTVTTLRWGIIYLLNNPEVQAKCQMEILDVFGNDIPDMGKMNQTPYVRATLSEIQRLANVLPWAIPHKTIEECNIGGYDIPVNTEIIPALGAVLFDPNVFESPKQFKPERFLDEEGKYRVMEEFRPFGLGPRVCLGERIARTELYLIFASLLQNFRFYLNRGDPIPVAERVIGGITAPPKPYATRVEYLGNRLIN
Protein DAF-9, isoform b
Refseq ID NP_001024903
Protein GI 71996285
UniProt ID H2KYS3
mRNA ID NM_001029732
Length 557
RefSeq Status REVIEWED
MPLVIGRFSSLSCERASIWCNMNISQSSWIKCRDWMAFALSHHIIMGIYLLILRNFLPQVVPDFEWQHYFMRVFIVHIIYIIISYFIRITRYPPGPPPMAVFGNSPFVNILTPEQTFLEYREIYGPIFTLHLSQPTIILAEYKTIQEALVKNGQQTSGRSSAESFVLFTGDRLNGDGVILAMRQKWKDMRHEISRFMNKWYGAPMDELVLHHTRCLEQELAKIAETKSLIDLRDPLAGAIANVIQQITIGRNYMYQDQEFQTQLRDINAVVKEIMTAEVFFVNCYPWLRYLPEGILRKWTNYKRSGFRLQQWFRTILEEHHVNRHQGDFMSHMIDLQESKQEQFRDLSIILTCGDMWTGGMETTVTTLRWGIIYLLNNPEVQAKCQMEILDVFGNDIPDMGKMNQTPYVRATLSEIQRLANVLPWAIPHKTIEECNIGGYDIPVNTEIIPALGAVLFDPNVFESPKQFKPERFLDEEGKYRVMEEFRPFGLGPRVCLGERIARTELYLIFASLLQNFRFYLNRGDPIPVAERVIGGITAPPKPYATRVEYLGNRLIN

Gene Information

Entrez Gene ID
180889
Gene Name
Protein DAF-9
Gene Symbol
daf-9
Species
Caenorhabditis elegans

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:WormBase C cytoplasm
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0043025 IDA:WormBase C neuronal cell body
GO:0048471 IDA:WormBase C perinuclear region of cytoplasm
GO:0047749 IEA:UniProtKB-EC F cholestanetriol 26-monooxygenase activity
GO:0020037 IEA:InterPro F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0007267 IMP:WormBase P cell-cell signaling
GO:0008203 IEA:UniProtKB-KW P cholesterol metabolic process
GO:0040024 IMP:WormBase P dauer larval development
GO:0030334 IMP:WormBase P regulation of cell migration

Domain Information

InterPro Annotations

Accession Description
IPR001128 Cytochrome P450
IPR002401 Cytochrome P450, E-class, group I
IPR017972 Cytochrome P450, conserved site

UniProt Annotations

Entry Information

Gene Name
Protein DAF-9
Protein Entry
DAF9_CAEEL
UniProt ID
H2KYS3
Species
C. elegans

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=a; IsoId=H2KYS3-1; Sequence=Displayed; Name=b; IsoId=H2KYS3-2; Sequence=VSP_045859;
Catalytic Activity 5-beta-cholestane-3-alpha,7-alpha,12-alpha- triol + 3 NADPH + 3 O(2) = (25R)-3-alpha,7-alpha,12-alpha- trihydroxy-5-beta-cholestan-26-oate + 3 NADP(+) + 4 H(2)O. {ECO:0000269|PubMed:16529801}.
Catalytic Activity 5-beta-cholestane-3-alpha,7-alpha,12-alpha- triol + NADPH + O(2) = (25R)-5-beta-cholestane-3-alpha,7-alpha,12- alpha,26-tetraol + NADP(+) + H(2)O. {ECO:0000269|PubMed:16529801}.
Function Sterol hydroxylase that catalyzes the successive oxidization of the side chains of the 3-keto steroids 4-cholesten- 3-one and lathosterone to form their carboxylic acid derivatives delta(4)-dafachronic acid and delta(7)-dafachronic acid respectively. Dafachronic acids bind directly to the nuclear hormone receptor (NHR) daf-12, suppressing dauer formation and inducing reproductive growth. {ECO:0000269|PubMed:11782415, ECO:0000269|PubMed:16529801, ECO:0000269|PubMed:22505847}.
Pathway Steroid hormone biosynthesis; dafachronic acid biosynthesis. {ECO:0000269|PubMed:16529801}.
Similarity Belongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular Location Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007889 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
71996277 RefSeq NP_741807 572 Protein DAF-9, isoform a
71996285 RefSeq NP_001024903 557 Protein DAF-9, isoform b

Identical Sequences to LMP007889 proteins

Reference Database Accession Length Protein Name
GI:71996277 EMBL CCD64552.1 572 Protein DAF-9, isoform a [Caenorhabditis elegans]
GI:71996285 EMBL CCD64553.1 557 Protein DAF-9, isoform b [Caenorhabditis elegans]
GI:71996285 GenBank AAL65132.1 557 DAF-9 isoform A [Caenorhabditis elegans]
GI:71996277 SwissProt H2KYS3.1 572 RecName: Full=Cytochrome P450 daf-9 [Caenorhabditis elegans]

Related Sequences to LMP007889 proteins

Reference Database Accession Length Protein Name
GI:71996285 EMBL CCD64552.1 572 Protein DAF-9, isoform a [Caenorhabditis elegans]
GI:71996277 EMBL CCD64553.1 557 Protein DAF-9, isoform b [Caenorhabditis elegans]
GI:71996277 GenBank AAL65132.1 557 DAF-9 isoform A [Caenorhabditis elegans]
GI:71996285 GenBank EFO82524.1 534 CRE-DAF-9 protein [Caenorhabditis remanei]
GI:71996285 PIR - 587 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans [Caenorhabditis elegans]
GI:71996277 PIR - 587 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans [Caenorhabditis elegans]
GI:71996285 RefSeq NP_741807.2 572 Protein DAF-9, isoform a [Caenorhabditis elegans]
GI:71996277 RefSeq NP_001024903.1 557 Protein DAF-9, isoform b [Caenorhabditis elegans]
GI:71996277 RefSeq XP_003117926.1 534 CRE-DAF-9 protein [Caenorhabditis remanei]
GI:71996285 RefSeq XP_003117926.1 534 CRE-DAF-9 protein [Caenorhabditis remanei]
GI:71996277 RefSeq XP_003416551.1 503 PREDICTED: cytochrome P450 3A4-like [Loxodonta africana]
GI:71996285 SwissProt H2KYS3.1 572 RecName: Full=Cytochrome P450 daf-9 [Caenorhabditis elegans]