Gene/Proteome Database (LMPD)

LMPD ID
LMP009675
Gene ID
838739
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
thiamin diphosphate-binding fold protein
Gene Symbol
AT1G21400
Synonyms
F24J8.4; F24J8_4
Alternate Names
thiamin diphosphate-binding fold protein
Chromosome
1
EC Number
1.2.4.4

Proteins

thiamin diphosphate-binding fold protein
Refseq ID NP_173562
Protein GI 15218984
UniProt ID Q9LPL5
mRNA ID NM_101992
Length 472
RefSeq Status REVIEWED
MAIWFARSKTLVSSLRHNLNLSTILIKRDYSHRPIFYTTSQLSSTAYLSPFGSLRHESTAVETQADHLVQQIDEVDAQELDFPGGKVGYTSEMKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTISSPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPVLIEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRSNARKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYPPGFHV

Gene Information

Entrez Gene ID
838739
Gene Name
thiamin diphosphate-binding fold protein
Gene Symbol
AT1G21400
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005739 IEA:UniProtKB-KW C mitochondrion
GO:0003863 IEA:UniProtKB-EC F 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0043617 IEP:UniProtKB P cellular response to sucrose starvation
GO:0009646 IEP:UniProtKB P response to absence of light
GO:0009744 IEP:UniProtKB P response to sucrose

KEGG Pathway Links

KEGG Pathway ID Description
ath01110 Biosynthesis of secondary metabolites
ath_M00036 Leucine degradation, leucine => acetoacetate + acetyl-CoA
ath01100 Metabolic pathways

REACTOME Pathway Links

REACTOME Pathway ID Description
6253683 Branched-chain amino acid catabolism

Domain Information

InterPro Annotations

Accession Description
IPR001017 Dehydrogenase, E1 component
IPR029061 Thiamin diphosphate-binding fold

UniProt Annotations

Entry Information

Gene Name
thiamin diphosphate-binding fold protein
Protein Entry
ODBA1_ARATH
UniProt ID
Q9LPL5
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2- methylpropanoyl)transferase] S-(2- methylpropanoyl)dihydrolipoyllysine + CO(2).
Cofactor Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000250};
Function The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity). Required during sugar starvation. {ECO:0000250, ECO:0000269|PubMed:11418132}.
Induction By dark treatment. Down-regulated by sucrose in a hexokinase dependent manner. Up-regulated by Leucine and its derivative alpha-keto acid (KIC). {ECO:0000269|PubMed:11418132}.
Miscellaneous Bound potassium ions stabilize the protein structure. {ECO:0000250}.
Similarity Belongs to the BCKDHA family. {ECO:0000305}.
Subcellular Location Mitochondrion matrix {ECO:0000250}.
Subunit Heterotetramer of alpha and beta chains. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009675 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15218984 RefSeq NP_173562 472 thiamin diphosphate-binding fold protein

Identical Sequences to LMP009675 proteins

Reference Database Accession Length Protein Name
GI:15218984 GenBank AAF87894.1 472 branched-chain alpha keto-acid dehydrogenase E1 - alpha subunit [Arabidopsis thaliana]
GI:15218984 GenBank AAM20466.1 472 branched-chain alpha keto-acid dehydrogenase, putative [Arabidopsis thaliana]
GI:15218984 GenBank AAN15588.1 472 branched-chain alpha keto-acid dehydrogenase, putative [Arabidopsis thaliana]
GI:15218984 GenBank AEE30099.1 472 thiamin diphosphate-binding fold protein [Arabidopsis thaliana]
GI:15218984 SwissProt Q9LPL5.1 472 RecName: Full=2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial; AltName: Full=Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; Short=BCKDE1A; Short=BCKDH E1-alpha; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP009675 proteins

Reference Database Accession Length Protein Name
GI:15218984 GenBank AAC69851.1 472 branched-chain alpha keto-acid dehydrogenase E1 alpha subunit, partial [Arabidopsis thaliana]
GI:15218984 GenBank AAW00649.1 472 Sequence 12 from patent US 6773917
GI:15218984 GenBank EFH66700.1 472 hypothetical protein ARALYDRAFT_313033 [Arabidopsis lyrata subsp. lyrata]
GI:15218984 GenBank EOA40298.1 476 hypothetical protein CARUB_v10009022mg [Capsella rubella]
GI:15218984 RefSeq XP_002890441.1 472 hypothetical protein ARALYDRAFT_313033 [Arabidopsis lyrata subsp. lyrata]
GI:15218984 RefSeq XP_006307400.1 476 hypothetical protein CARUB_v10009022mg [Capsella rubella]