Gene/Proteome Database (LMPD)
LMPD ID
LMP009678
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
branched chain alpha-keto acid dehydrogenase E1 beta
Gene Symbol
Synonyms
DARK INDUCIBLE 4; DIN4
Alternate Names
branched chain alpha-keto acid dehydrogenase E1 beta
Chromosome
3
EC Number
1.2.4.4
Summary
branched chain alpha-keto acid dehydrogenase E1 beta
Orthologs
Proteins
| branched chain alpha-keto acid dehydrogenase E1 beta | |
|---|---|
| Refseq ID | NP_187954 |
| Protein GI | 15231242 |
| UniProt ID | Q9LDY2 |
| mRNA ID | NM_112191 |
| Length | 358 |
| RefSeq Status | REVIEWED |
| MAAALVRRFCRGSSFPVSGHGYRMLSTVENVSESGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLAERFGKSRVFNTPLCEQGIVGFGIGLAAMGNRVIAEIQFADYIFPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAKGLLLSSIRDPNPVVFFEPKWLYRQAVEDVPEDDYMIPLSEAEVMREGSDITLVGWGAQLTIMEQACLDAENEGISCELIDLKTLIPWDKEIVETSVRKTGRLLISHEAPVTGGFGAEIAATIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIRSTVNY | |
Gene Information
Entrez Gene ID
Gene Name
branched chain alpha-keto acid dehydrogenase E1 beta
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005739 | IEA:UniProtKB-KW | C | mitochondrion |
| GO:0003863 | IEA:UniProtKB-EC | F | 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity |
| GO:0043617 | IEP:UniProtKB | P | cellular response to sucrose starvation |
| GO:0009646 | IEP:UniProtKB | P | response to absence of light |
| GO:0009744 | IEP:UniProtKB | P | response to sucrose |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath01110 | Biosynthesis of secondary metabolites |
| ath_M00036 | Leucine degradation, leucine => acetoacetate + acetyl-CoA |
| ath01100 | Metabolic pathways |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| 6253683 | Branched-chain amino acid catabolism |
Domain Information
UniProt Annotations
Entry Information
Gene Name
branched chain alpha-keto acid dehydrogenase E1 beta
Protein Entry
ODBB2_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2- methylpropanoyl)transferase] S-(2- methylpropanoyl)dihydrolipoyllysine + CO(2). |
| Developmental Stage | Barely detected in non senescent green leaves, accumulated slightly at the early stage of leaf senescence and strongly expressed at the late stage of leaf senescence. {ECO:0000269|PubMed:10681595}. |
| Function | The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity). Required during sugar starvation and acts under the control of a sugar-sensing mechanism involving Ser/Thr kinases and phosphatases. {ECO:0000250, ECO:0000269|PubMed:11080291, ECO:0000269|PubMed:11917081}. |
| Induction | By dark treatment. Down-regulated by sucrose in a hexokinase dependent manner. Up-regulated by Leucine and its derivative alpha-keto acid (KIC). {ECO:0000269|PubMed:10681595, ECO:0000269|PubMed:11080291, ECO:0000269|PubMed:11418132, ECO:0000269|PubMed:11917081}. |
| Subcellular Location | Mitochondrion matrix {ECO:0000250}. |
| Subunit | Heterotetramer of alpha and beta chains. {ECO:0000250}. |
| Tissue Specificity | Expressed in the non-photosynthetic organs such as siliques, flowers and roots. {ECO:0000269|PubMed:10681595}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP009678 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 15231242 | RefSeq | NP_187954 | 358 | branched chain alpha-keto acid dehydrogenase E1 beta |
Identical Sequences to LMP009678 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15231242 | DBBJ | BAB01752.1 | 358 | branched chain alpha-keto acid dehydrogenase E1 beta subunit [Arabidopsis thaliana] |
| GI:15231242 | DBBJ | BAF01133.1 | 358 | branched chain alpha-keto acid dehydrogenase E1 beta subunit [Arabidopsis thaliana] |
| GI:15231242 | GenBank | AAF35281.1 | 358 | branched chain alpha-keto acid dehydrogenase E1 beta subunit [Arabidopsis thaliana] |
| GI:15231242 | GenBank | ABD85160.1 | 358 | At3g13450 [Arabidopsis thaliana] |
| GI:15231242 | GenBank | AEE75357.1 | 358 | branched chain alpha-keto acid dehydrogenase E1 beta [Arabidopsis thaliana] |
| GI:15231242 | SwissProt | Q9LDY2.1 | 358 | RecName: Full=2-oxoisovalerate dehydrogenase subunit beta 2, mitochondrial; AltName: Full=Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; Short=BCKDE1B; Short=BCKDH E1-beta; AltName: Full=Protein DARK INDUCIBLE 4; Flags: Precursor [Arabidopsis thaliana] |
Related Sequences to LMP009678 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15231242 | GenBank | EFH61226.1 | 360 | hypothetical protein ARALYDRAFT_478729 [Arabidopsis lyrata subsp. lyrata] |
| GI:15231242 | GenBank | ESQ48668.1 | 357 | hypothetical protein EUTSA_v10021009mg [Eutrema salsugineum] |
| GI:15231242 | GenBank | KFK38675.1 | 357 | hypothetical protein AALP_AA3G145900 [Arabis alpina] |
| GI:15231242 | RefSeq | XP_002884967.1 | 360 | hypothetical protein ARALYDRAFT_478729 [Arabidopsis lyrata subsp. lyrata] |
| GI:15231242 | RefSeq | XP_006407215.1 | 357 | hypothetical protein EUTSA_v10021009mg [Eutrema salsugineum] |
| GI:15231242 | RefSeq | XP_009106875.1 | 357 | PREDICTED: 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Brassica rapa] |