Gene/Proteome Database (LMPD)

LMPD ID
LMP009681
Gene ID
841132
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
Gene Symbol
3BETAHSD/D1
Synonyms
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1; 3BETAHSD/D1; AT3BETAHSD/D; AT3BETAHSD/D1; T3F24.9; T3F24_9
Alternate Names
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
Chromosome
1
EC Number
1.1.1.170
Summary
Encodes an enzyme with 3&#946;-hydroxysteroid dehydrogenase/C4-decarboxylase activity <i>in vitro</i>. The activity of the enzyme was determined using microsomal extracts of yeast overexpressing the <i>Arabidopsis</i> gene. Cytosolic fractions failed to be associated to the activity, leading to the speculation that the enzyme is membrane-bound.
Orthologs
View orthologs and multiple alignments for 3BETAHSD/D1

Proteins

3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
Refseq ID NP_564502
Protein GI 18401656
UniProt ID Q9FX01
mRNA ID NM_103623
Length 382
RefSeq Status REVIEWED
MVMEVTETERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYVSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRLIYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRPSSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEVCAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGPYGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQNQPKTEEKMKVFGSKKID
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
Refseq ID NP_849779
Protein GI 30694041
UniProt ID Q9FX01
mRNA ID NM_179448
Length 439
RefSeq Status REVIEWED
MVMEVTETERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYVSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRLIYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRPSSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEVCAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGPYGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQNQPKTEVTETIQWKKQTLIAIVILITLYHNFVATTGSSSVIITAVSKVLLVSSIFMFINGILPEKMKVFGSKKID

Gene Information

Entrez Gene ID
841132
Gene Name
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
Gene Symbol
3BETAHSD/D1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:TAIR C membrane
GO:0003854 IEA:InterPro F 3-beta-hydroxy-delta5-steroid dehydrogenase activity
GO:0047012 IDA:TAIR F sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity
GO:0016126 IEA:UniProtKB-KW P sterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01100 Metabolic pathways
ath00100 Steroid biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
6254049 Androgen biosynthesis
6254052 Glucocorticoid biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR002225 3-beta hydroxysteroid dehydrogenase/isomerase
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
Protein Entry
HSDD1_ARATH
UniProt ID
Q9FX01
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9FX01-1; Sequence=Displayed; Name=2; IsoId=Q9FX01-2; Sequence=VSP_037002; Note=May be due to a competing acceptor splice site. No experimental confirmation available. Ref.1 (AAY28502) and Ref.6 (AAM62504) sequences differ from that shown due to erroneous initiation (Translation N-terminally extended). {ECO:0000305};
Catalytic Activity A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)(+) = a 3-oxosteroid + CO(2) + NAD(P)H.
Caution Lacks one transmembrane, which is a conserved feature of the family. {ECO:0000305}.
Domain The RETICULON domain is partial in comparison with the other paralogs.
Pathway Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.
Sequence Caution Sequence=AAM62504.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAY28502.1; Type=Erroneous initiation; Evidence={ECO:0000305};
Similarity Belongs to the 3-beta-HSD family. {ECO:0000305}.
Similarity Contains 1 reticulon domain. {ECO:0000255|PROSITE- ProRule:PRU00170}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009681 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
30694041 RefSeq NP_849779 439 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
18401656 RefSeq NP_564502 382 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1

Identical Sequences to LMP009681 proteins

Reference Database Accession Length Protein Name
GI:30694041 DBBJ BAC42142.1 439 putative 3-beta-hydroxysteroid dehydrogenase [Arabidopsis thaliana]
GI:30694041 GenBank AAG11424.1 439 Similar to steriod dehydrogenase [Arabidopsis thaliana]
GI:30694041 GenBank AAO50699.1 439 putative 3-beta-hydroxysteroid dehydrogenase [Arabidopsis thaliana]
GI:18401656 GenBank AEE32152.1 382 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 [Arabidopsis thaliana]
GI:30694041 GenBank AEE32153.1 439 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 [Arabidopsis thaliana]
GI:30694041 SwissProt Q9FX01.1 439 RecName: Full=3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1; Short=At3BETAHSD/D1; AltName: Full=4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase isoform 1-1; AltName: Full=Reticulon-like protein B24; Short=AtRTNLB24; AltName: Full=Sterol-4-alpha-carboxylate 3-dehydrogenase 1, decarboxylating [Arabidopsis thaliana]

Related Sequences to LMP009681 proteins

Reference Database Accession Length Protein Name
GI:18401656 DBBJ BAC42142.1 439 putative 3-beta-hydroxysteroid dehydrogenase [Arabidopsis thaliana]
GI:18401656 GenBank AAG11424.1 439 Similar to steriod dehydrogenase [Arabidopsis thaliana]
GI:18401656 GenBank AAM62504.1 380 3-beta-hydroxysteroid dehydrogenase, putative [Arabidopsis thaliana]
GI:18401656 GenBank AAO50699.1 439 putative 3-beta-hydroxysteroid dehydrogenase [Arabidopsis thaliana]
GI:18401656 GenBank AAY28502.1 380 4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase isoform 1-1 [Arabidopsis thaliana]
GI:30694041 GenBank EFH67606.1 439 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 [Arabidopsis lyrata subsp. lyrata]
GI:18401656 GenBank AEE32153.1 439 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 [Arabidopsis thaliana]
GI:30694041 GenBank EOA40438.1 440 hypothetical protein CARUB_v10009164mg [Capsella rubella]
GI:30694041 RefSeq XP_002891347.1 439 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 [Arabidopsis lyrata subsp. lyrata]
GI:30694041 RefSeq XP_010500257.1 439 PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1-like isoform X1 [Camelina sativa]
GI:30694041 RefSeq XP_010461531.1 439 PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 isoform X1 [Camelina sativa]
GI:30694041 RefSeq XP_010479134.1 439 PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1-like isoform X1 [Camelina sativa]