Gene/Proteome Database (LMPD)

LMPD ID
LMP009682
Gene ID
817166
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
Gene Symbol
3BETAHSD/D2
Synonyms
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2; 3BETAHSD/D2; AT3BETAHSD/D; AT3BETAHSD/D2; T1D16.10; T1D16_10
Alternate Names
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
Chromosome
2
EC Number
1.1.1.170
Summary
Encodes an enzyme with 3&#946;-hydroxysteroid dehydrogenase/C4-decarboxylase activity <i>in vitro</i>. The activity of the enzyme was determined using microsomal extracts of yeast overexpressing the <i>Arabidopsis</i> gene. Cytosolic fractions failed to be associated to the activity, leading to the speculation that the enzyme is membrane-bound.
Orthologs
View orthologs and multiple alignments for 3BETAHSD/D2

Proteins

3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
Refseq ID NP_001031422
Protein GI 79323109
UniProt ID Q67ZE1
mRNA ID NM_001036345
Length 473
RefSeq Status REVIEWED
MAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVSTKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPYGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSKREGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVASVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGNDWSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSFACKHKSDVYEKLFGSKKHD
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
Refseq ID NP_180194
Protein GI 42569340
UniProt ID Q67ZE1
mRNA ID NM_128183
Length 564
RefSeq Status REVIEWED
MSPAATETERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQYISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVSTKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPYGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSKREGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVASVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGNDWSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSFACKHKSDVYEKLFGSKKHD

Gene Information

Entrez Gene ID
817166
Gene Name
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
Gene Symbol
3BETAHSD/D2
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:TAIR C membrane
GO:0003854 IEA:InterPro F 3-beta-hydroxy-delta5-steroid dehydrogenase activity
GO:0047012 IDA:TAIR F sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity
GO:0016126 IEA:UniProtKB-KW P sterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01100 Metabolic pathways
ath00100 Steroid biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
6254041 Synthesis of bile acids and bile salts
6254045 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol

Domain Information

InterPro Annotations

Accession Description
IPR002225 3-beta hydroxysteroid dehydrogenase/isomerase
IPR016040 NAD(P)-binding domain
IPR003388 Reticulon

UniProt Annotations

Entry Information

Gene Name
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
Protein Entry
HSDD2_ARATH
UniProt ID
Q67ZE1
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q67ZE1-1; Sequence=Displayed; Note=Constructed according to the conserved gene model. No experimental confirmation available.; Name=2; IsoId=Q67ZE1-2; Sequence=VSP_037004, VSP_037005; Note=May be due to an intron retention. No experimental confirmation available.; Name=3; IsoId=Q67ZE1-3; Sequence=VSP_037003; Note=May be due to a competing acceptor splice site. No experimental confirmation available.;
Catalytic Activity A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)(+) = a 3-oxosteroid + CO(2) + NAD(P)H.
Pathway Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.
Sequence Caution Sequence=BX818951; Type=Frameshift; Positions=283; Evidence={ECO:0000305};
Similarity Belongs to the 3-beta-HSD family. {ECO:0000305}.
Similarity Contains 1 reticulon domain. {ECO:0000255|PROSITE- ProRule:PRU00170}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009682 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
42569340 RefSeq NP_180194 564 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
79323109 RefSeq NP_001031422 473 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2

Identical Sequences to LMP009682 proteins

Reference Database Accession Length Protein Name
GI:79323109 DBBJ BAD43940.1 473 3-beta-hydroxysteroid dehydrogenase [Arabidopsis thaliana]
GI:42569340 GenBank AEC07814.1 564 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [Arabidopsis thaliana]
GI:79323109 GenBank AEC07815.1 473 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [Arabidopsis thaliana]
GI:42569340 SwissProt Q67ZE1.2 564 RecName: Full=3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2; Short=At3BETAHSD/D2; AltName: Full=4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase isoform 1-2; AltName: Full=Reticulon-like protein B19; Short=AtRTNLB19; AltName: Full=Sterol-4-alpha-carboxylate 3-dehydrogenase 2, decarboxylating [Arabidopsis thaliana]

Related Sequences to LMP009682 proteins

Reference Database Accession Length Protein Name
GI:42569340 GenBank EFH55210.1 564 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [Arabidopsis lyrata subsp. lyrata]
GI:79323109 GenBank EFH55210.1 564 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [Arabidopsis lyrata subsp. lyrata]
GI:79323109 GenBank AEC07814.1 564 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [Arabidopsis thaliana]
GI:79323109 RefSeq NP_180194.2 564 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [Arabidopsis thaliana]
GI:79323109 RefSeq XP_002878951.1 564 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [Arabidopsis lyrata subsp. lyrata]
GI:42569340 RefSeq XP_002878951.1 564 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [Arabidopsis lyrata subsp. lyrata]
GI:42569340 RefSeq XP_010417410.1 564 PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2-like isoform X1 [Camelina sativa]
GI:42569340 RefSeq XP_010429646.1 565 PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2-like [Camelina sativa]
GI:42569340 RefSeq XP_010472654.1 564 PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 isoform X1 [Camelina sativa]
GI:79323109 RefSeq XP_010472655.1 564 PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 isoform X1 [Camelina sativa]
GI:42569340 RefSeq XP_010472655.1 564 PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 isoform X1 [Camelina sativa]
GI:79323109 SwissProt Q67ZE1.2 564 RecName: Full=3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2; Short=At3BETAHSD/D2; AltName: Full=4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase isoform 1-2; AltName: Full=Reticulon-like protein B19; Short=AtRTNLB19; AltName: Full=Sterol-4-alpha-carboxylate 3-dehydrogenase 2, decarboxylating [Arabidopsis thaliana]