Gene/Proteome Database (LMPD)

LMPD ID
LMP009688
Gene ID
816244
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
Gene Symbol
HMG2
Synonyms
3-hydroxy-3-methylglutaryl-CoA reductase 2; 3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE 2; F5J6.1; F5J6_1; HMG2; HMGR2
Alternate Names
3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
Chromosome
2
EC Number
1.1.1.34
Summary
Encodes a 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) that is involved in the synthesis of sterol and triterpenoid compounds.
Orthologs
View orthologs and multiple alignments for HMG2

Proteins

3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
Refseq ID NP_179329
Protein GI 15227821
UniProt ID P43256
mRNA ID NM_127292
Length 562
RefSeq Status REVIEWED
MEDLRRRFPTKKNGEEISNVAVDPPLRKASDALPLPLYLTNTFFLSLFFATVYFLLSRWREKIRNSTPLHVVDLSEICALIGFVASFIYLLGFCGIDLIFRSSSDDDVWVNDGMIPCNQSLDCREVLPIKPNSVDPPRESELDSVEDEEIVKLVIDGTIPSYSLETKLGDCKRAAAIRREAVQRITGKSLTGLPLEGFDYNSILGQCCEMPVGYVQIPVGIAGPLLLDGVEYSVPMATTEGCLVASTNRGFKAIHLSGGAFSVLVKDAMTRAPVVRFPSARRAALVMFYLQDPSNFERLSLIFNKSSRFARLQSITCTIAGRNLYPRFACSTGDAMGMNMVSKGVQNVLDFVKSEFPDMDVIGISGNYCSDKKASAVNWIEGRGKHVVCEAFIKAEIVEKVLKTSVEALVELNTLKNLVGSAMAGSLGGFNAHSSNIVSAVFIATGQDPAQNVESSHCMTMILPDGDDLHISVSMPCIEVGTVGGGTQLASQAACLNLLGVKGSNNEKPGSNAQQLARIVAGSVLAGELSLMSAIAAGQLVKSHMKYNRSSRDIGPSSQVNR

Gene Information

Entrez Gene ID
816244
Gene Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
Gene Symbol
HMG2
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0043231 IDA:TAIR C intracellular membrane-bounded organelle
GO:0050661 IEA:InterPro F NADP binding
GO:0004420 IEA:UniProtKB-EC F hydroxymethylglutaryl-CoA reductase (NADPH) activity
GO:0042282 ISS:TAIR F hydroxymethylglutaryl-CoA reductase activity
GO:0015936 IEA:InterPro P coenzyme A metabolic process
GO:0008299 TAS:TAIR P isoprenoid biosynthetic process
GO:0016126 IMP:TAIR P sterol biosynthetic process
GO:0016104 IMP:TAIR P triterpenoid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath_M00095 C5 isoprenoid biosynthesis, mevalonate pathway
ath00900 Terpenoid backbone biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
6254329 Activation of gene expression by SREBF (SREBP)
6254036 Cholesterol biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR023282 Hydroxymethylglutaryl-CoA reductase, N-terminal
IPR002202 Hydroxymethylglutaryl-CoA reductase, class I/II
IPR009023 Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain
IPR023074 Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic domain
IPR023076 Hydroxymethylglutaryl-CoA reductase, class I/II, conserved site
IPR009029 Hydroxymethylglutaryl-CoA reductase, class I/II, substrate-binding domain
IPR004554 Hydroxymethylglutaryl-CoA reductase, eukaryotic/arcaheal type

UniProt Annotations

Entry Information

Gene Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
Protein Entry
HMDH2_ARATH
UniProt ID
P43256
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity (R)-mevalonate + CoA + 2 NADP(+) = (S)-3- hydroxy-3-methylglutaryl-CoA + 2 NADPH. {ECO:0000255|PROSITE- ProRule:PRU10003}.
Disruption Phenotype No visible phenotype under normal growth conditions, but 25% lower levels in triterpenoids content and 15% lower levels in sterol content. Hmg1 and hmg2 double mutants are lethal during male gametophyte development. {ECO:0000269|PubMed:14871314, ECO:0000269|PubMed:17917299, ECO:0000269|PubMed:19363204}.
Enzyme Regulation Regulated at the post-translational level in response to alterations of the sphingolipid and the sterol biosynthetic pathways. {ECO:0000269|PubMed:19041104}.
Function Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants. {ECO:0000269|PubMed:14871314, ECO:0000269|PubMed:17917299, ECO:0000269|PubMed:19041104, ECO:0000269|PubMed:19363204}.
Induction Not regulated by myriocin, squalestatin or terbinafine. {ECO:0000269|PubMed:19041104}.
Pathway Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Similarity Belongs to the HMG-CoA reductase family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:8302869}; Multi-pass membrane protein {ECO:0000269|PubMed:8302869}.
Tissue Specificity Restricted to young seedlings, roots, and inflorescences. Expressed in root tips, shoot apex, secretory zone of the stigma, microspores, mature pollen grains, gynoecium vascular tissue and fertilized ovules. {ECO:0000269|PubMed:19363204, ECO:0000269|PubMed:7780305, ECO:0000269|PubMed:8302869}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009688 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15227821 RefSeq NP_179329 562 3-hydroxy-3-methylglutaryl-coenzyme A reductase 2

Identical Sequences to LMP009688 proteins

Reference Database Accession Length Protein Name
GI:15227821 GenBank AAA67317.1 562 3-hydroxy-3-methylglutaryl-CoA reductase [Arabidopsis thaliana]
GI:15227821 GenBank AAL15311.1 562 At2g17370/F15M4.1 [Arabidopsis thaliana]
GI:15227821 GenBank AAN28869.1 562 At2g17370/F15M4.1 [Arabidopsis thaliana]
GI:15227821 GenBank AEC06618.1 562 3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 [Arabidopsis thaliana]
GI:15227821 SwissProt P43256.1 562 RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase 2; Short=AtHMGR2; Short=HMG-CoA reductase 2 [Arabidopsis thaliana]

Related Sequences to LMP009688 proteins

Reference Database Accession Length Protein Name
GI:15227821 GenBank EFH60312.1 564 3-hydroxy-3-methylglutaryl-CoA reductase 2 [Arabidopsis lyrata subsp. lyrata]
GI:15227821 GenBank EOA30238.1 563 hypothetical protein CARUB_v10013360mg [Capsella rubella]
GI:15227821 RefSeq XP_002884053.1 564 3-hydroxy-3-methylglutaryl-CoA reductase 2 [Arabidopsis lyrata subsp. lyrata]
GI:15227821 RefSeq XP_006297340.1 563 hypothetical protein CARUB_v10013360mg [Capsella rubella]
GI:15227821 RefSeq XP_010467508.1 559 PREDICTED: 3-hydroxy-3-methylglutaryl-coenzyme A reductase 2-like [Camelina sativa]
GI:15227821 RefSeq XP_010489363.1 552 PREDICTED: 3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 [Camelina sativa]