Gene/Proteome Database (LMPD)

LMPD ID
LMP009692
Gene ID
817165
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
3-ketoacyl-CoA synthase 10
Gene Symbol
KCS10
Synonyms
3-ketoacyl-CoA synthase 10; FDH; FIDDLEHEAD; KCS10; T1D16.11; T1D16_11
Alternate Names
3-ketoacyl-CoA synthase 10
Chromosome
2
EC Number
2.3.1.199
Summary
epidermis-specific, encodes KCS10, a putative 3-ketoacyl-CoA synthase. probably involved in the synthesis of long-chain lipids found in the cuticle.
Orthologs
View orthologs and multiple alignments for KCS10

Proteins

3-ketoacyl-CoA synthase 10
Refseq ID NP_180193
Protein GI 15225270
UniProt ID Q570B4
mRNA ID NM_128182
Length 550
RefSeq Status REVIEWED
MGRSNEQDLLSTEIVNRGIEPSGPNAGSPTFSVRVRRRLPDFLQSVNLKYVKLGYHYLINHAVYLATIPVLVLVFSAEVGSLSREEIWKKLWDYDLATVIGFFGVFVLTACVYFMSRPRSVYLIDFACYKPSDEHKVTKEEFIELARKSGKFDEETLGFKKRILQASGIGDETYVPRSISSSENITTMKEGREEASTVIFGALDELFEKTRVKPKDVGVLVVNCSIFNPTPSLSAMVINHYKMRGNILSYNLGGMGCSAGIIAIDLARDMLQSNPNSYAVVVSTEMVGYNWYVGSDKSMVIPNCFFRMGCSAVMLSNRRRDFRHAKYRLEHIVRTHKAADDRSFRSVYQEEDEQGFKGLKISRDLMEVGGEALKTNITTLGPLVLPFSEQLLFFAALLRRTFSPAAKTSTTTSFSTSATAKTNGIKSSSSDLSKPYIPDYKLAFEHFCFHAASKVVLEELQKNLGLSEENMEASRMTLHRFGNTSSSGIWYELAYMEAKESVRRGDRVWQIAFGSGFKCNSVVWKAMRKVKKPTRNNPWVDCINRYPVPL

Gene Information

Entrez Gene ID
817165
Gene Name
3-ketoacyl-CoA synthase 10
Gene Symbol
KCS10
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:TAIR C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:TAIR C membrane
GO:0016747 IEA:InterPro F transferase activity, transferring acyl groups other than amino-acyl groups
GO:0006633 IEA:UniProtKB-UniPathway P fatty acid biosynthetic process
GO:0009409 IEP:TAIR P response to cold
GO:0080167 IEP:TAIR P response to karrikin
GO:0009416 IEP:TAIR P response to light stimulus

KEGG Pathway Links

KEGG Pathway ID Description
ath01110 Biosynthesis of secondary metabolites
ath_M00415 Fatty acid biosynthesis, elongation, endoplasmic reticulum
M00415 Fatty acid biosynthesis, elongation, endoplasmic reticulum
ath00062 Fatty acid elongation

Domain Information

InterPro Annotations

Accession Description
IPR013747 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal
IPR013601 FAE1/Type III polyketide synthase-like protein
IPR016039 Thiolase-like
IPR016038 Thiolase-like, subgroup
IPR012392 Very-long-chain 3-ketoacyl-CoA synthase

UniProt Annotations

Entry Information

Gene Name
3-ketoacyl-CoA synthase 10
Protein Entry
KCS10_ARATH
UniProt ID
Q570B4
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity A very-long-chain acyl-CoA + malonyl-CoA = CoA + a very-long-chain 3-oxoacyl-CoA + CO(2).
Developmental Stage Expressed in prefusion carpel abaxial and adaxial epidermis. Accumulates in ovule primordia, but restricted to chalaza in mature ovules. Also present in cells of stigmatic papillae, at the margins of ovules, and around the embryo sac. {ECO:0000269|PubMed:10559443, ECO:0000269|PubMed:10655527}.
Disruption Phenotype In additions to several malformations due to organ fusion, plants lacking FDH demonstrate an enhanced cell wall permeability, reduced trichome formation, and are female sterile. {ECO:0000269|PubMed:10559443}.
Function Contributes to cuticular wax and suberin biosynthesis. Prevents the postgenital fusion of epiderm cells in organs in contact, as well as ectopic pollen hydration and germination. Required during ovules formation. May regulate an epidermis- specific developmental program during gynoecial ontogeny. {ECO:0000269|PubMed:10559443, ECO:0000269|PubMed:10655527, ECO:0000269|PubMed:1644226, ECO:0000269|PubMed:8416837, ECO:0000269|PubMed:9299123, ECO:0000269|PubMed:9611177}.
Induction Repressed by herbicides such as flufenacet and benfuresate. {ECO:0000269|PubMed:12916765}.
Miscellaneous Called 'FIDDLEHEAD' because of the shape of mutants, in which adhering floral buds cause curling of inflorescence, resulting in structures reminiscent of fern fiddlehead.
Pathway Lipid metabolism; fatty acid biosynthesis.
Sequence Caution Sequence=BAD94049.1; Type=Erroneous initiation; Evidence={ECO:0000305};
Similarity Belongs to the chalcone/stilbene synthases family. {ECO:0000305}.
Similarity Contains 1 FAE (fatty acid elongase) domain. {ECO:0000305}.
Subcellular Location Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
Tissue Specificity Mostly expressed in epidermal cells of floral and vegetative meristems and, to a lower extent, of leaves and coleoptiles, especially in young tissues. Also present in trichomes and phloem. {ECO:0000269|PubMed:10559443, ECO:0000269|PubMed:10655527}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009692 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15225270 RefSeq NP_180193 550 3-ketoacyl-CoA synthase 10

Identical Sequences to LMP009692 proteins

Reference Database Accession Length Protein Name
GI:15225270 GenBank AAN31115.1 550 At2g26250/T1D16.11 [Arabidopsis thaliana]
GI:15225270 GenBank AAN86193.1 550 putative beta-ketoacyl-CoA synthase FIDDLEHEAD [Arabidopsis thaliana]
GI:15225270 GenBank AAX04403.1 550 Sequence 2 from patent US 6849435
GI:15225270 GenBank AEC07813.1 550 3-ketoacyl-CoA synthase 10 [Arabidopsis thaliana]
GI:15225270 GenBank AGD03360.1 550 Sequence 816 from patent US 8343764
GI:15225270 SwissProt Q570B4.2 550 RecName: Full=3-ketoacyl-CoA synthase 10; Short=KCS-10; AltName: Full=Protein FIDDLEHEAD; AltName: Full=Very long-chain fatty acid condensing enzyme 10; Short=VLCFA condensing enzyme 10 [Arabidopsis thaliana]

Related Sequences to LMP009692 proteins

Reference Database Accession Length Protein Name
GI:15225270 GenBank AAF73976.1 550 fiddlehead protein [Arabidopsis thaliana]
GI:15225270 GenBank AAF73979.1 550 fiddlehead protein [Arabidopsis thaliana]
GI:15225270 GenBank AAF73980.1 550 fiddlehead protein [Arabidopsis thaliana]
GI:15225270 GenBank AAE84924.1 550 Sequence 8 from patent US 6307128
GI:15225270 GenBank EFH57030.1 550 predicted protein [Arabidopsis lyrata subsp. lyrata]
GI:15225270 RefSeq XP_002880771.1 550 predicted protein [Arabidopsis lyrata subsp. lyrata]