Gene/Proteome Database (LMPD)
LMPD ID
LMP009719
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
3-oxoacyl-[acyl-carrier-protein] synthase II
Gene Symbol
Synonyms
ARABIDOPSIS BETA-KETOACYL-ACP SYNTHETASE 2; ATKAS2; BETA-KETOACYL-ACP SYNTHETASE 2; F25A4.7; F25A4_7; FAB1; fatty acid biosynthesis 1; KAS2
Alternate Names
3-oxoacyl-[acyl-carrier-protein] synthase II
Chromosome
1
EC Number
2.3.1.41
Summary
Encodes a plastidic beta-ketoacyl-ACP synthase II, involved in fatty acid elongation from 16:0-ACP to 18:0-ACP. Homozygous knock-out mutants are embryo lethal, indicating early embryo development is sensitive to elevated 16:0.
Orthologs
Proteins
| 3-oxoacyl-[acyl-carrier-protein] synthase II | |
|---|---|
| Refseq ID | NP_001185400 |
| Protein GI | 334183918 |
| UniProt ID | Q9C9P4 |
| mRNA ID | NM_001198471 |
| Length | 541 |
| RefSeq Status | REVIEWED |
| MVGASSSYASPLCTWFVAACMSVSHGGGDSRQAVALQSGGRSRRRRQLSKCSVASGSASIQALVTSCLDFGPCTHYNNNNALSSLFGSNSVSLNRNQRRLNRAASSGGAMAVMEMEKEAAVNKKPPTEQRRVVVTGMGVETSLGHDPHTFYENLLQGNSGISQIENFDCSEFPTRIAGEIKSFSTEGWVAPKLSKRMDKFMLYLLTAGKKALADGGVTDEVMAEFDKTKCGVLIGSAMGGMKVFYDAIEALRISYKKMNPFCVPFATTNMGSAMLAMDLGWMGPNYSISTACATSNFCILNSANHIIKGEADVMLCGGSDAVIIPIGLGGFVACRALSQRNNDPTKASRPWDTNRDGFVMGEGAGVLLLEELEHAKKRGATIYAEFLGGSFTCDAYHMTEPHPDGAGVILCIERALASAGISKEQINYINAHATSTHAGDIKEYQALAHCFGQNPELKVNSTKSMIGHLLGAAGAVEAVATVQAIRTGWVHPNINLENPDSGVDTKLLVGPKKERLDIKAALSNSFGFGGHNSSIIFAPYK | |
Gene Information
Entrez Gene ID
Gene Name
3-oxoacyl-[acyl-carrier-protein] synthase II
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0009507 | IDA:TAIR | C | chloroplast |
| GO:0009570 | IDA:TAIR | C | chloroplast stroma |
| GO:0009536 | ISS:TAIR | C | plastid |
| GO:0004315 | IDA:TAIR | F | 3-oxoacyl-[acyl-carrier-protein] synthase activity |
| GO:0009631 | IMP:UniProtKB | P | cold acclimation |
| GO:0009793 | IMP:UniProtKB | P | embryo development ending in seed dormancy |
| GO:0006633 | IDA:TAIR | P | fatty acid biosynthetic process |
| GO:0006636 | TAS:TAIR | P | unsaturated fatty acid biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath00780 | Biotin metabolism |
| ath00061 | Fatty acid biosynthesis |
| ath_M00083 | Fatty acid biosynthesis, elongation |
| ath01212 | Fatty acid metabolism |
Domain Information
UniProt Annotations
Entry Information
Gene Name
3-oxoacyl-[acyl-carrier-protein] synthase II
Protein Entry
KASC2_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Acyl-[acyl-carrier-protein] + malonyl-[acyl- carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]. {ECO:0000269|PubMed:12232312}. |
| Developmental Stage | First observed during the transition from the late globular to the early heart embryo stages. Later observed during heart, tropedo, and cotyledonary embryo stages. In seedlings, observed in the shoot apex and stomatal guard cells. In adult plants, expressed in inflorescences. In flowers, strongly present in styles and pollen grains. {ECO:0000269|PubMed:18506098}. |
| Disruption Phenotype | Lethal when homozygous due to embryo abortion before the torpedo stage. Converts temperate oilseed composition (rich in unsaturated 18-carbon fatty acids) to that of a palm-like tropical oil (enriched in saturated 16-carbon fatty acids). {ECO:0000269|PubMed:17360594, ECO:0000269|PubMed:18506098}. |
| Function | Essential protein that catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-16 and C-16 to unsaturated C-18 fatty acids. Confers resistance to low temperatures by maintaining chloroplast membranes integrity. Involved in the regulation of fatty acids ratios during seed metabolism. Required for embryo development, especially at the transition from the globular to the heart stage. {ECO:0000269|PubMed:12148534, ECO:0000269|PubMed:12232312, ECO:0000269|PubMed:12242349, ECO:0000269|PubMed:17360594, ECO:0000269|PubMed:18506098, ECO:0000269|PubMed:9046588}. |
| Sequence Caution | Sequence=AAD55280.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=AAL06498.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAL06498.1; Type=Frameshift; Positions=38, 328; Evidence={ECO:0000305}; |
| Similarity | Belongs to the beta-ketoacyl-ACP synthases family. {ECO:0000305}. |
| Subcellular Location | Plastid, chloroplast stroma {ECO:0000269|PubMed:18431481, ECO:0000269|PubMed:20061580}. |
| Subunit | Homodimer. {ECO:0000250}. |
| Tissue Specificity | Mostly expressed in siliques, and, to a lower extent, in leaves, stems, flower buds, and flowers. {ECO:0000269|PubMed:18506098}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP009719 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 334183918 | RefSeq | NP_001185400 | 541 | 3-oxoacyl-[acyl-carrier-protein] synthase II |
Identical Sequences to LMP009719 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:334183918 | EMBL | CBN70761.1 | 541 | unnamed protein product [Arabidopsis thaliana] |
| GI:334183918 | EMBL | CBN69153.1 | 541 | unnamed protein product [Arabidopsis thaliana] |
| GI:334183918 | GenBank | AEE35655.1 | 541 | 3-oxoacyl-[acyl-carrier-protein] synthase II [Arabidopsis thaliana] |
| GI:334183918 | GenBank | AEE35656.1 | 541 | 3-oxoacyl-[acyl-carrier-protein] synthase II [Arabidopsis thaliana] |
| GI:334183918 | GenBank | AEE35657.1 | 541 | 3-oxoacyl-[acyl-carrier-protein] synthase II [Arabidopsis thaliana] |
| GI:334183918 | GenBank | AGE99616.1 | 541 | Sequence 3 from patent US 8361173 |
Related Sequences to LMP009719 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:334183918 | EMBL | CBN70899.1 | 541 | unnamed protein product [Arabidopsis thaliana] |
| GI:334183918 | EMBL | CBN70914.1 | 562 | unnamed protein product [Arabidopsis thaliana] |
| GI:334183918 | EMBL | CBN69291.1 | 541 | unnamed protein product [Arabidopsis thaliana] |
| GI:334183918 | GenBank | AAL91174.1 | 541 | putative 3-ketoacyl-ACP synthase [Arabidopsis thaliana] |
| GI:334183918 | GenBank | EFH65261.1 | 543 | hypothetical protein ARALYDRAFT_476635 [Arabidopsis lyrata subsp. lyrata] |
| GI:334183918 | RefSeq | XP_002889002.1 | 543 | hypothetical protein ARALYDRAFT_476635 [Arabidopsis lyrata subsp. lyrata] |