Gene/Proteome Database (LMPD)
LMPD ID
LMP009842
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
alpha-dioxygenase
Gene Symbol
Synonyms
alpha-dioxygenase 1; ALPHA-DOX1; DIOX1; DOX1; PADOX-1; plant alpha dioxygenase 1; T13O15.6; T13O15_6
Alternate Names
alpha-dioxygenase
Chromosome
3
EC Number
1.14.99.-
Summary
Encodes an alpha-dioxygenase involved in protection against oxidative stress and cell death. Induced in response to Salicylic acid and oxidative stress. Independent of NPR1 in induction by salicylic acid.
Orthologs
Proteins
| alpha-dioxygenase | |
|---|---|
| Refseq ID | NP_186791 |
| Protein GI | 15232116 |
| UniProt ID | Q9SGH6 |
| mRNA ID | NM_111008 |
| Length | 639 |
| RefSeq Status | REVIEWED |
| MKVITSLISSILLKFIHKDFHEIYARMSLLDRFLLLIVHGVDKMVPWHKLPVFLGLTYLEVRRHLHQQYNLLNVGQTPTGIRFDPANYPYRTADGKFNDPFNEGVGSQNSFFGRNCPPVDQKSKLRRPDPMVVATKLLGRKKFIDTGKQFNMIAASWIQFMIHDWIDHLEDTHQIELVAPKEVASKCPLSSFRFLKTKEVPTGFFEIKTGSQNIRTPWWDSSVIYGSNSKTLDRVRTYKDGKLKISEETGLLLHDEDGLAISGDIRNSWAGVSALQALFIKEHNAVCDALKDEDDDLEDEDLYRYARLVTSAVVAKIHTIDWTVQLLKTDTLLAGMRANWYGLLGKKFKDSFGHAGSSILGGVVGMKKPQNHGVPYSLTEDFTSVYRMHSLLPDQLHILDIDDVPGTNKSLPLIQEISMRDLIGRKGEETMSHIGFTKLMVSMGHQASGALELMNYPMWLRDIVPHDPNGQARPDHVDLAALEIYRDRERSVPRYNEFRRSMFMIPITKWEDLTEDEEAIEVLDDVYDGDVEELDLLVGLMAEKKIKGFAISETAFYIFLIMATRRLEADRFFTSDFNETIYTKKGLEWVNTTESLKDVIDRHYPDMTDKWMNSESAFSVWDSPPLTKNPIPLYLRIPS | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0012511 | IDA:TAIR | C | monolayer-surrounded lipid storage body |
| GO:0020037 | IEA:InterPro | F | heme binding |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0016702 | IDA:TAIR | F | oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen |
| GO:0004601 | IEA:UniProtKB-KW | F | peroxidase activity |
| GO:1902609 | IMP:TAIR | P | (R)-2-hydroxy-alpha-linolenic acid biosynthetic process |
| GO:0008219 | IEP:TAIR | P | cell death |
| GO:0071732 | IEP:UniProtKB | P | cellular response to nitric oxide |
| GO:0034614 | IEP:UniProtKB | P | cellular response to reactive oxygen species |
| GO:0071446 | IEP:UniProtKB | P | cellular response to salicylic acid stimulus |
| GO:0042742 | IEP:UniProtKB | P | defense response to bacterium |
| GO:0050832 | IDA:TAIR | P | defense response to fungus |
| GO:0001561 | IDA:TAIR | P | fatty acid alpha-oxidation |
| GO:0006629 | TAS:TAIR | P | lipid metabolic process |
| GO:0031408 | IEA:UniProtKB-KW | P | oxylipin biosynthetic process |
| GO:0009626 | IEA:UniProtKB-KW | P | plant-type hypersensitive response |
| GO:0009737 | IMP:UniProtKB | P | response to abscisic acid |
| GO:0006979 | IMP:TAIR | P | response to oxidative stress |
| GO:0009751 | IEP:TAIR | P | response to salicylic acid |
| GO:0009627 | IMP:UniProtKB | P | systemic acquired resistance |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath00592 | alpha-Linolenic acid metabolism |
| ko00592 | alpha-Linolenic acid metabolism |
BIOCYC Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=18 uM for linolenic acid (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; KM=17 uM for linoleic acid (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; KM=13 uM for oleic acid (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; KM=11 uM for palmitic acid (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; Vmax=119 nmol/min/mg enzyme with linolenic acid as substrate (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; Vmax=111 nmol/min/mg enzyme with linoleic acid as substrate (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; Vmax=91 nmol/min/mg enzyme with oleic acid as substrate (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; Vmax=46 nmol/min/mg enzyme with palmitic acid as substrate (at pH 8 and 30 degrees Celsius) {ECO:0000269|PubMed:10455113}; |
| Disruption Phenotype | Slightly higher accumulation of Pseudomonas syringae pv. tomato DC3000 in infected leaves. Partially impaired systemic acquired resistance (SAR) in response to incompatible interaction. When associated with LOX1 disruption; hypersensitivity to the growth-inhibitory effect of abscisic acid (ABA) accompanied by an accumulation of ABA-inducible marker genes. {ECO:0000269|PubMed:22199234}. |
| Function | Alpha-dioxygenase that catalyzes the primary oxygenation of fatty acids into oxylipins. Mediates a protection against oxidative stress and cell death, probably by generating some lipid-derived molecules. Promotes local and systemic plant defense in a salicylic acid (SA)-dependent manner, including the establishment of systemic acquired resistance (SAR) in response to incompatible interaction. Involved in a negative regulation of abscisic acid (ABA)-mediated signaling pathway. {ECO:0000269|PubMed:10455113, ECO:0000269|PubMed:12060227, ECO:0000269|PubMed:22199234}. |
| Induction | Induced by salicylic acid (SA) and by some chemicals generating reactive oxygen species (ROS, e.g. nitric oxide (NO), intracellular superoxide and singlet oxygen) such as sodium nitropruside (SNP), paraquat and rose bengal (RB). Accumulates locally, at the infection site, in response to both incompatible and compatible bacterial pathogens (e.g. Pseudomonas syringae pv. tomato DC3000) in a SA-dependent manner, with a faster response during hypersensitive reactions. {ECO:0000269|PubMed:12060227}. |
| Similarity | Belongs to the peroxidase family. {ECO:0000255|PROSITE-ProRule:PRU00298}. |
| Tissue Specificity | Expressed in roots (epiderm), mature flowers (e.g. anthers) and senescing leaves. {ECO:0000269|PubMed:12060227, ECO:0000269|PubMed:17369372}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP009842 (as displayed in Record Overview)
Identical Sequences to LMP009842 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15232116 | GenBank | AAL87742.1 | 639 | alphaDOX1 fatty acid dioxygenase [Arabidopsis thaliana] |
| GI:15232116 | GenBank | ABP06342.1 | 639 | Sequence 39 from patent US 7179627 |
| GI:15232116 | GenBank | ADA08492.1 | 639 | Sequence 39 from patent US 7601816 |
| GI:15232116 | GenBank | ADT60257.1 | 639 | Sequence 1404 from patent US 7847156 |
| GI:15232116 | GenBank | AEE73664.1 | 639 | alpha-dioxygenase [Arabidopsis thaliana] |
| GI:15232116 | SwissProt | Q9SGH6.1 | 639 | RecName: Full=Alpha-dioxygenase 1; Short=Alpha DOX1; AltName: Full=Fatty acid dioxygenase AlphaDOX1; AltName: Full=Pathogen-induced oxygenase; AltName: Full=Plant alpha dioxygenase 1; Flags: Precursor [Arabidopsis thaliana] |
Related Sequences to LMP009842 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15232116 | GenBank | EFH58443.1 | 639 | alpha-dioxygenase 1 [Arabidopsis lyrata subsp. lyrata] |
| GI:15232116 | GenBank | EOA30078.1 | 645 | hypothetical protein CARUB_v10013184mg, partial [Capsella rubella] |
| GI:15232116 | PDB | 4HHR | 652 | Chain A, Crystal Structure Of Fatty Acid Alpha-dioxygenase (arabidopsis Thaliana) |
| GI:15232116 | PDB | 4HHS | 652 | Chain A, Crystal Structure Of Fatty Acid Alpha-dioxygenase (arabidopsis Thaliana) |
| GI:15232116 | RefSeq | XP_002882184.1 | 639 | alpha-dioxygenase 1 [Arabidopsis lyrata subsp. lyrata] |
| GI:15232116 | RefSeq | XP_010463524.1 | 639 | PREDICTED: alpha-dioxygenase 1 [Camelina sativa] |