Gene/Proteome Database (LMPD)

LMPD ID
LMP009851
Gene ID
840476
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
annexin 1
Gene Symbol
ANNAT1
Synonyms
ANNEXIN; annexin 1; ANNEXIN A1; ATOXY5; F14D7.2; F14D7_2; OXY5
Alternate Names
annexin 1
Chromosome
1
Summary
Encodes a member of the annexin gene family, a diverse, multigene family of calcium-dependent, membrane-binding proteins. The protein was determined to have peroxidase activity. This activity is thought to be dependent on the presence of post-translational modifications (most likely phosphorylation). The protein was shown to be present as a mixture of monomer and homodimer. The homodimerization seems to be dependent on the presence of Ca2+ or H2O2. The dimerization was prevented by the addition of DTT, β-mercaptoethanol and TCEP. Annat1 mRNA is expressed in flowers, roots,leaves and stems and is most abundant in stems. mRNA levels are increased in response to oxidative stress. Developmental expression patterns suggest a role in Golgi-mediated polysaccharide secretion.
Orthologs
View orthologs and multiple alignments for ANNAT1

Proteins

annexin 1
Refseq ID NP_174810
Protein GI 15220216
UniProt ID Q9SYT0
mRNA ID NM_103274
Length 317
RefSeq Status REVIEWED
MATLKVSDSVPAPSDDAEQLRTAFEGWGTNEDLIISILAHRSAEQRKVIRQAYHETYGEDLLKTLDKELSNDFERAILLWTLEPGERDALLANEATKRWTSSNQVLMEVACTRTSTQLLHARQAYHARYKKSLEEDVAHHTTGDFRKLLVSLVTSYRYEGDEVNMTLAKQEAKLVHEKIKDKHYNDEDVIRILSTRSKAQINATFNRYQDDHGEEILKSLEEGDDDDKFLALLRSTIQCLTRPELYFVDVLRSAINKTGTDEGALTRIVTTRAEIDLKVIGEEYQRRNSIPLEKAITKDTRGDYEKMLVALLGEDDA

Gene Information

Entrez Gene ID
840476
Gene Name
annexin 1
Gene Symbol
ANNAT1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0048046 IDA:TAIR C apoplast
GO:0005618 IDA:TAIR C cell wall
GO:0009507 IDA:TAIR C chloroplast
GO:0009570 IDA:TAIR C chloroplast stroma
GO:0005829 IDA:TAIR C cytosol
GO:0016020 IDA:TAIR C membrane
GO:0005739 IDA:TAIR C mitochondrion
GO:0005886 IDA:TAIR C plasma membrane
GO:0009506 IDA:TAIR C plasmodesma
GO:0009579 IDA:TAIR C thylakoid
GO:0005774 IDA:TAIR C vacuolar membrane
GO:0005773 IDA:TAIR C vacuole
GO:0005524 IDA:TAIR F ATP binding
GO:0005509 ISS:TAIR F calcium ion binding
GO:0005544 IEA:UniProtKB-KW F calcium-dependent phospholipid binding
GO:0005507 IDA:TAIR F copper ion binding
GO:0004601 IDA:TAIR F peroxidase activity
GO:0042803 IDA:TAIR F protein homodimerization activity
GO:0008270 IDA:TAIR F zinc ion binding
GO:0070588 IDA:TAIR P calcium ion transmembrane transport
GO:0055114 IDA:GOC P oxidation-reduction process
GO:0071435 IDA:TAIR P potassium ion export
GO:0009737 IMP:TAIR P response to abscisic acid
GO:0046686 IEP:TAIR P response to cadmium ion
GO:0009409 IEP:TAIR P response to cold
GO:0009408 IEP:TAIR P response to heat
GO:0006970 IMP:TAIR P response to osmotic stress
GO:0006979 IGI:TAIR P response to oxidative stress
GO:0009651 IEP:TAIR P response to salt stress
GO:0009414 IEP:TAIR P response to water deprivation

Domain Information

InterPro Annotations

Accession Description
IPR001464 Annexin
IPR018252 Annexin repeat, conserved site
IPR009118 Annexin, plant
IPR018502 Annexin_repeat

UniProt Annotations

Entry Information

Gene Name
annexin 1
Protein Entry
ANXD1_ARATH
UniProt ID
Q9SYT0
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties pH dependence: Active at pH 7.0 and 9.0, but not at pH 5.5. {ECO:0000269|PubMed:16153598};
Developmental Stage Expressed in the elongation zone of the root and in the root cap in germinating seedlings. Expressed later in the internal cells of the root and in the epidermal cells and the vascular tissue of the hypocotyl. By day 7, expressed in the initiating trichomes on leaf primordia and in the vasculature of hypocotyl and cotyledon. At the transition to reproductive growth (day 14), expressed in the vasculature, epidermis, basal mesophyll cells and pith meristem of leaves. {ECO:0000269|PubMed:11457958, ECO:0000269|PubMed:15368128}.
Disruption Phenotype Plants are hypersensitive to osmotic stress and abscisic acid (ABA) during germination and early seedling growth. {ECO:0000269|PubMed:15161963}.
Domain A pair of annexin repeats may form one binding site for calcium and phospholipid.
Function Has a peroxidase activity. May act in counteracting oxidative stress. May also mediate regulated, targeted secretion of Golgi-derived vesicles during seedling development. {ECO:0000269|PubMed:15368128, ECO:0000269|PubMed:16153598}.
Induction Up-regulated by cold, dehydration, salt, osmotic and oxidative stresses. Up-regulated by abscisic acid (ABA) and salicylic acid (SA). {ECO:0000269|PubMed:15161963, ECO:0000269|PubMed:16531057, ECO:0000269|PubMed:8855345}.
Miscellaneous Binds lipids at millimolar calcium concentration.
Ptm Phosphorylated.
Similarity Belongs to the annexin (TC 1.A.31.1) family. {ECO:0000305}.
Similarity Contains 4 annexin repeats. {ECO:0000305}.
Subcellular Location Cytoplasm, cytosol. Membrane. Note=translocate from cytosol to membrane upon salt treatment; this translocation is calcium dependent.
Subunit Monomer and homodimer. {ECO:0000269|PubMed:16153598}.
Tissue Specificity Ubiquitous. Most abundant in stems. {ECO:0000269|PubMed:15368128, ECO:0000269|PubMed:8855345}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009851 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15220216 RefSeq NP_174810 317 annexin 1

Identical Sequences to LMP009851 proteins

Reference Database Accession Length Protein Name
GI:15220216 EMBL CBC30815.1 317 unnamed protein product [Arabidopsis thaliana]
GI:15220216 GenBank EFH67463.1 317 ANNAT1 [Arabidopsis lyrata subsp. lyrata]
GI:15220216 GenBank ADT60786.1 317 Sequence 2462 from patent US 7847156
GI:15220216 GenBank AEE31825.1 317 annexin 1 [Arabidopsis thaliana]
GI:15220216 GenBank AFL64811.1 317 Sequence 74 from patent US 8188339
GI:15220216 RefSeq XP_002891204.1 317 ANNAT1 [Arabidopsis lyrata subsp. lyrata]

Related Sequences to LMP009851 proteins

Reference Database Accession Length Protein Name
GI:15220216 EMBL CAW56132.1 317 unnamed protein product [Arabidopsis thaliana]
GI:15220216 GenBank AAC49472.1 317 annexin-like protein [Arabidopsis thaliana]
GI:15220216 PDB 1YCN 317 Chain A, X-Ray Structure Of Annexin From Arabidopsis Thaliana Gene At1g35720
GI:15220216 PDB 1YCN 317 Chain B, X-Ray Structure Of Annexin From Arabidopsis Thaliana Gene At1g35720
GI:15220216 PDB 2Q4C 317 Chain A, Ensemble Refinement Of The Protein Crystal Structure Of Annexin From Arabidopsis Thaliana Gene At1g35720
GI:15220216 PDB 2Q4C 317 Chain B, Ensemble Refinement Of The Protein Crystal Structure Of Annexin From Arabidopsis Thaliana Gene At1g35720