Gene/Proteome Database (LMPD)

LMPD ID
LMP009947
Gene ID
833406
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
purple acid phosphatase 26
Gene Symbol
PAP26
Synonyms
ATPAP26; PAP26; purple acid phosphatase 26; PURPLE ACID PHOSPHATASE 26; T5E15.10; T5E15_10
Alternate Names
purple acid phosphatase 26
Chromosome
5
EC Number
3.1.3.2

Proteins

purple acid phosphatase 26
Refseq ID NP_198334
Protein GI 15242870
UniProt ID Q949Y3
mRNA ID NM_122874
Length 475
RefSeq Status REVIEWED
MNHLVIISVFLSSVLLLYRGESGITSSFIRSEWPAVDIPLDHHVFKVPKGYNAPQQVHITQGDYDGKAVIISWVTPDEPGSSQVHYGAVQGKYEFVAQGTYHNYTFYKYKSGFIHHCLVSDLEHDTKYYYKIESGESSREFWFVTPPHVHPDASYKFGIIGDMGQTFNSLSTLEHYMESGAQAVLFLGDLSYADRYQYNDVGVRWDSWGRFVERSTAYQPWLWSAGNHEVDYMPYMGEVTPFRNYLQRYTTPYLASKSSSPLWYAVRRASAHIIVLSSYSPFVKYTPQWHWLSEELTRVDREKTPWLIVLMHVPIYNSNEAHFMEGESMRAAFEEWFVQHKVDVIFAGHVHAYERSYRISNVRYNVSSGDRYPVPDKSAPVYITVGDGGNQEGLAGRFTEPQPDYSAFREASYGHSTLDIKNRTHAIYHWNRNDDGKKVATDEFVLHNQYWGKNIRRRKLKKHYIRSVVGGWIAT

Gene Information

Entrez Gene ID
833406
Gene Name
purple acid phosphatase 26
Gene Symbol
PAP26
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:TAIR C cytosol
GO:0009505 IDA:TAIR C plant-type cell wall
GO:0005773 IDA:TAIR C vacuole
GO:0003993 IMP:TAIR F acid phosphatase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0004601 IEA:UniProtKB-KW F peroxidase activity
GO:0016311 IMP:GOC P dephosphorylation
GO:0042744 IEA:UniProtKB-KW P hydrogen peroxide catabolic process
GO:0055062 IMP:TAIR P phosphate ion homeostasis

Domain Information

InterPro Annotations

Accession Description
IPR004843 Calcineurin-like phosphoesterase domain, apaH type
IPR025733 Iron/zinc purple acid phosphatase-like C-terminal domain
IPR029052 Metallo-dependent phosphatase-like
IPR015914 Purple acid phosphatase, N-terminal
IPR008963 Purple acid phosphatase-like, N-terminal

UniProt Annotations

Entry Information

Gene Name
purple acid phosphatase 26
Protein Entry
PPA26_ARATH
UniProt ID
Q949Y3
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Absorption: Abs(max)=520 nm {ECO:0000269|PubMed:16963519}; Kinetic parameters: KM=0.8 mM for phosphoenolpyruvate (for the phosphatase activity) {ECO:0000269|PubMed:16963519}; pH dependence: Optimum pH is 5.6 for the phosphatase activity and 8.8 for the peroxidase activity. {ECO:0000269|PubMed:16963519};
Catalytic Activity 2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O.
Catalytic Activity A phosphate monoester + H(2)O = an alcohol + phosphate.
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250}; Note=Binds 1 Fe cation per subunit. {ECO:0000250};
Cofactor Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250}; Note=Binds 1 zinc ion per subunit. {ECO:0000250};
Enzyme Regulation Activated by Mg(2+), Co(2+), Mn(2+) and Ba(2+). Inhibited by Fe(2+), Cu(2+), Zn(2+), NaF, molybdate, arsenate, vanadate and inorganic phosphate. No effect of tartrate, Asp, Gln, glutathione, Asn, ascorbic acid and phosphite.
Function Metallo-phosphoesterase involved in phosphate metabolism. Acid phosphatase activity with phosphoenolpyruvate, inorganic pyrophosphate, phenyl-phosphate and p-nitrophenyl- phosphate as the most effective substrates. No activity with phytic acid, phosphocholine or bis-p-nitrophenyl-phosphate. Has a peroxidase activity at alkaline pH. {ECO:0000269|PubMed:16963519}.
Induction Not induced at the transcription level by phosphate starvation, but accumulation of the protein in starved shoots. {ECO:0000269|PubMed:16963519}.
Ptm Glycosylated. {ECO:0000269|PubMed:16963519}.
Similarity Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. {ECO:0000305}.
Subcellular Location Vacuole {ECO:0000269|PubMed:15215502, ECO:0000269|PubMed:15539469, ECO:0000269|PubMed:16963519}.
Subunit Homodimer.
Tissue Specificity Expressed in roots, stems, leaves, flowers and siliques. {ECO:0000269|PubMed:16244908}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009947 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15242870 RefSeq NP_198334 475 purple acid phosphatase 26

Identical Sequences to LMP009947 proteins

Reference Database Accession Length Protein Name
GI:15242870 GenBank AAK92747.1 475 putative acid phosphatase [Arabidopsis thaliana]
GI:15242870 GenBank AAM14354.1 475 putative acid phosphatase [Arabidopsis thaliana]
GI:15242870 gnl TAIR 475 purple acid phosphatase 26 [Arabidopsis thaliana]
GI:15242870 SwissProt Q949Y3.1 475 RecName: Full=Bifunctional purple acid phosphatase 26; Includes: RecName: Full=Acid phosphatase; Includes: RecName: Full=Peroxidase; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP009947 proteins

Reference Database Accession Length Protein Name
GI:15242870 GenBank AAW29950.1 475 putative purple acid phosphatase [Arabidopsis thaliana]
GI:15242870 GenBank EFH46667.1 475 ATPAP26/PAP26 [Arabidopsis lyrata subsp. lyrata]
GI:15242870 RefSeq XP_002870408.1 475 ATPAP26/PAP26 [Arabidopsis lyrata subsp. lyrata]
GI:15242870 RefSeq XP_010435389.1 475 PREDICTED: bifunctional purple acid phosphatase 26 isoform X1 [Camelina sativa]
GI:15242870 RefSeq XP_010440687.1 475 PREDICTED: bifunctional purple acid phosphatase 26-like isoform X1 [Camelina sativa]
GI:15242870 RefSeq XP_010440688.1 475 PREDICTED: bifunctional purple acid phosphatase 26-like isoform X1 [Camelina sativa]