Gene/Proteome Database (LMPD)

LMPD ID
LMP010018
Gene ID
843128
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Gene Symbol
PIS1
Synonyms
ATPIS; ATPIS1; PHOSPHATIDYLINOSITOL SYNTHASE; phosphatidylinositol synthase 1; PIS1; T23K23.15; T23K23_15
Alternate Names
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Chromosome
1
EC Number
2.7.8.11
Summary
phosphatidylinositol synthase 1
Orthologs
View orthologs and multiple alignments for PIS1

Proteins

CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Refseq ID NP_176967
Protein GI 15220618
UniProt ID Q8LBA6
mRNA ID NM_105470
Length 227
RefSeq Status REVIEWED
MAKKERPRPEKLSVYLYIPNIVGYMRVLLNCVAFAVCFSNKPLFSVLYFFSFCCDAVDGWVARRFNQVSTFGAVLDMVTDRVSTACLLVILSQIYRPSLVFLSLLALDIASHWLQMYSTFLAGKSSHKDVKDSTSWLFRLYYGNRIFMCYCCVSCEVLYIILLLIAKNQSENLLNVVVATLTQISPLSFLLALTLFGWSMKQTINVIQMKTAADVCVLYDIEKQQKP

Gene Information

Entrez Gene ID
843128
Gene Name
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Gene Symbol
PIS1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IDA:TAIR C Golgi apparatus
GO:0005783 IDA:TAIR C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:TAIR C membrane
GO:0003881 IDA:TAIR F CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0008654 IDA:TAIR P phospholipid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath00564 Glycerophospholipid metabolism
ath00562 Inositol phosphate metabolism
ath01100 Metabolic pathways

REACTOME Pathway Links

REACTOME Pathway ID Description
6254315 Synthesis of PI

Domain Information

InterPro Annotations

Accession Description
IPR000462 CDP-alcohol phosphatidyltransferase
IPR014387 CDP-diacylglycerol-inositol 3-phosphatidyltransferase, eukaryote

UniProt Annotations

Entry Information

Gene Name
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Protein Entry
PIS1_ARATH
UniProt ID
Q8LBA6
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=400 uM for myo-inositol {ECO:0000269|PubMed:14563413}; KM=10 uM for CDP-diacylglycerol {ECO:0000269|PubMed:14563413};
Catalytic Activity CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl-1D-myo-inositol. {ECO:0000269|PubMed:10411624}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:10411624, ECO:0000269|PubMed:10809447}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:10411624, ECO:0000269|PubMed:10809447};
Function Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme. {ECO:0000269|PubMed:10411624, ECO:0000269|PubMed:10809447, ECO:0000269|PubMed:11985617, ECO:0000269|PubMed:14563413}.
Similarity Belongs to the CDP-alcohol phosphatidyltransferase class-I family. {ECO:0000305}.
Subcellular Location Membrane; Multi-pass membrane protein.
Tissue Specificity Expressed in stems, flowers, shoots and roots. Present in epidermal tissues. {ECO:0000269|PubMed:10809447}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010018 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15220618 RefSeq NP_176967 227 CDP-diacylglycerol--inositol 3-phosphatidyltransferase

Identical Sequences to LMP010018 proteins

Reference Database Accession Length Protein Name
GI:15220618 EMBL CAA04172.1 227 phosphatidylinositol synthase [Arabidopsis thaliana]
GI:15220618 GenBank AAG52009.1 227 phosphatidylinositol synthase (PIS1); 53044-51460 [Arabidopsis thaliana]
GI:15220618 GenBank AAM98188.1 227 phosphatidylinositol synthase PIS1 [Arabidopsis thaliana]
GI:15220618 GenBank ACH13335.1 227 Sequence 2359 from patent US 7396979
GI:15220618 GenBank AEE34734.1 227 CDP-diacylglycerol--inositol 3-phosphatidyltransferase [Arabidopsis thaliana]
GI:15220618 SwissProt Q8LBA6.2 227 RecName: Full=CDP-diacylglycerol--inositol 3-phosphatidyltransferase 1; AltName: Full=Phosphatidylinositol synthase 1; Short=AtPIS1; Short=PI synthase 1; Short=PtdIns synthase 1 [Arabidopsis thaliana]

Related Sequences to LMP010018 proteins

Reference Database Accession Length Protein Name
GI:15220618 GenBank AAM64877.1 227 phosphatidylinositol synthase PIS1 [Arabidopsis thaliana]
GI:15220618 GenBank ACH13337.1 227 Sequence 2361 from patent US 7396979
GI:15220618 GenBank AEL85485.1 227 Sequence 1350 from patent US 7989676
GI:15220618 GenBank AFX28441.1 227 Sequence 9501 from patent US 8299318
GI:15220618 GenBank AFX48804.1 227 Sequence 50637 from patent US 8299318
GI:15220618 GenBank AGF13692.1 227 Sequence 13111 from patent US 8362325