Gene/Proteome Database (LMPD)
LMPD ID
LMP010018
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Gene Symbol
Synonyms
ATPIS; ATPIS1; PHOSPHATIDYLINOSITOL SYNTHASE; phosphatidylinositol synthase 1; PIS1; T23K23.15; T23K23_15
Alternate Names
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Chromosome
1
EC Number
2.7.8.11
Summary
phosphatidylinositol synthase 1
Orthologs
Proteins
| CDP-diacylglycerol--inositol 3-phosphatidyltransferase | |
|---|---|
| Refseq ID | NP_176967 |
| Protein GI | 15220618 |
| UniProt ID | Q8LBA6 |
| mRNA ID | NM_105470 |
| Length | 227 |
| RefSeq Status | REVIEWED |
| MAKKERPRPEKLSVYLYIPNIVGYMRVLLNCVAFAVCFSNKPLFSVLYFFSFCCDAVDGWVARRFNQVSTFGAVLDMVTDRVSTACLLVILSQIYRPSLVFLSLLALDIASHWLQMYSTFLAGKSSHKDVKDSTSWLFRLYYGNRIFMCYCCVSCEVLYIILLLIAKNQSENLLNVVVATLTQISPLSFLLALTLFGWSMKQTINVIQMKTAADVCVLYDIEKQQKP | |
Gene Information
Entrez Gene ID
Gene Name
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IDA:TAIR | C | endoplasmic reticulum |
| GO:0005794 | IDA:TAIR | C | Golgi apparatus |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0016020 | IDA:TAIR | C | membrane |
| GO:0003881 | IDA:TAIR | F | CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0008654 | IDA:TAIR | P | phospholipid biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath00564 | Glycerophospholipid metabolism |
| ath00562 | Inositol phosphate metabolism |
| ath01100 | Metabolic pathways |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| 6254315 | Synthesis of PI |
Domain Information
UniProt Annotations
Entry Information
Gene Name
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Protein Entry
PIS1_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=400 uM for myo-inositol {ECO:0000269|PubMed:14563413}; KM=10 uM for CDP-diacylglycerol {ECO:0000269|PubMed:14563413}; |
| Catalytic Activity | CDP-diacylglycerol + myo-inositol = CMP + phosphatidyl-1D-myo-inositol. {ECO:0000269|PubMed:10411624}. |
| Cofactor | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:10411624, ECO:0000269|PubMed:10809447}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:10411624, ECO:0000269|PubMed:10809447}; |
| Function | Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme. {ECO:0000269|PubMed:10411624, ECO:0000269|PubMed:10809447, ECO:0000269|PubMed:11985617, ECO:0000269|PubMed:14563413}. |
| Similarity | Belongs to the CDP-alcohol phosphatidyltransferase class-I family. {ECO:0000305}. |
| Subcellular Location | Membrane; Multi-pass membrane protein. |
| Tissue Specificity | Expressed in stems, flowers, shoots and roots. Present in epidermal tissues. {ECO:0000269|PubMed:10809447}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010018 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 15220618 | RefSeq | NP_176967 | 227 | CDP-diacylglycerol--inositol 3-phosphatidyltransferase |
Identical Sequences to LMP010018 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15220618 | EMBL | CAA04172.1 | 227 | phosphatidylinositol synthase [Arabidopsis thaliana] |
| GI:15220618 | GenBank | AAG52009.1 | 227 | phosphatidylinositol synthase (PIS1); 53044-51460 [Arabidopsis thaliana] |
| GI:15220618 | GenBank | AAM98188.1 | 227 | phosphatidylinositol synthase PIS1 [Arabidopsis thaliana] |
| GI:15220618 | GenBank | ACH13335.1 | 227 | Sequence 2359 from patent US 7396979 |
| GI:15220618 | GenBank | AEE34734.1 | 227 | CDP-diacylglycerol--inositol 3-phosphatidyltransferase [Arabidopsis thaliana] |
| GI:15220618 | SwissProt | Q8LBA6.2 | 227 | RecName: Full=CDP-diacylglycerol--inositol 3-phosphatidyltransferase 1; AltName: Full=Phosphatidylinositol synthase 1; Short=AtPIS1; Short=PI synthase 1; Short=PtdIns synthase 1 [Arabidopsis thaliana] |
Related Sequences to LMP010018 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15220618 | GenBank | AAM64877.1 | 227 | phosphatidylinositol synthase PIS1 [Arabidopsis thaliana] |
| GI:15220618 | GenBank | ACH13337.1 | 227 | Sequence 2361 from patent US 7396979 |
| GI:15220618 | GenBank | AEL85485.1 | 227 | Sequence 1350 from patent US 7989676 |
| GI:15220618 | GenBank | AFX28441.1 | 227 | Sequence 9501 from patent US 8299318 |
| GI:15220618 | GenBank | AFX48804.1 | 227 | Sequence 50637 from patent US 8299318 |
| GI:15220618 | GenBank | AGF13692.1 | 227 | Sequence 13111 from patent US 8362325 |