Gene/Proteome Database (LMPD)

LMPD ID
LMP010021
Gene ID
835203
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
ceramide kinase
Gene Symbol
AT5G51290
Synonyms
MWD22.24; MWD22_24
Alternate Names
ceramide kinase
Chromosome
5
EC Number
2.7.1.138

Proteins

ceramide kinase
Refseq ID NP_568756
Protein GI 42568457
UniProt ID Q6USK2
mRNA ID NM_124508
Length 608
RefSeq Status REVIEWED
MEEGRDDEYCSFSNSGDRDGGLSGCFFLDHVGQVLLSRNHDGLSWKCLDSSDCEGTTCLGIIICENSETEIKFSDIYAVEFVSYGLVHSPKLGLRHAKECFRERLLNTQEMYRFTVHGFQSSPKEPCLWNLAAFTFGHMDLQTCQSWMDQLNYSLIKEVERPRNLLVFVHPKSGKGNGSKVWETVSKIFIRAKVNTKVIVTERAGHAFDVMASIQNKELHTYDGIIAVGGDGFFNEILNGYLLSRLKVPLPPSPSDSFNSVQSRGSSSVPEPGDEVHETDQKEHYPLLPDSVQEVMNFRTVNGSCEGIEDPDHPFSSERPRFGLIPAGSTDAIVMCTTGARDPVTSALHIILGRKLFLDAMQVVRWKTASTSTIEPYIRYAASFAGYGFYGDVISESEKYRWMGPKRYDYVGTKIFLKHRSYEAEVMFEEAESENSKASLHTRSKTWPFRNTTRSEKILCRANCKICNSKVGWNSASTTLNPCPEKTRWCRTKGRFLSIGAAVMSNRNERAPDGLVVDAHLSDGFLHLILIKDCSRPKYLWHLTELAKRGGEPLNFEFVEYHKTRAFTFTSFGEESVWNLDGEIFEAHQLSAQVLRGLIPLFASGPEI

Gene Information

Entrez Gene ID
835203
Gene Name
ceramide kinase
Gene Symbol
AT5G51290
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0003951 IEA:InterPro F NAD+ kinase activity
GO:0005509 IDA:UniProtKB F calcium ion binding
GO:0001729 IDA:TAIR F ceramide kinase activity
GO:0004143 IEA:InterPro F diacylglycerol kinase activity
GO:0008219 IMP:TAIR P cell death
GO:0006672 IDA:TAIR P ceramide metabolic process
GO:0046834 IDA:GOC P lipid phosphorylation
GO:0007205 IEA:InterPro P protein kinase C-activating G-protein coupled receptor signaling pathway
GO:0043067 IMP:TAIR P regulation of programmed cell death

KEGG Pathway Links

KEGG Pathway ID Description
ath00600 Sphingolipid metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-5129 sphingolipid biosynthesis (plants)

REACTOME Pathway Links

REACTOME Pathway ID Description
6254321 Glycosphingolipid metabolism
6254192 Sphingolipid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR016064 ATP-NAD kinase-like domain
IPR001206 Diacylglycerol kinase, catalytic domain

UniProt Annotations

Entry Information

Gene Name
ceramide kinase
Protein Entry
CERK_ARATH
UniProt ID
Q6USK2
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=24.4 uM for C8 ceramide {ECO:0000269|PubMed:14563678}; pH dependence: Optimum pH is 8.2 (at 30 degrees Celsius). {ECO:0000269|PubMed:14563678};
Catalytic Activity ATP + ceramide = ADP + ceramide 1-phosphate. {ECO:0000269|PubMed:14563678}.
Cofactor Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:14563678};
Disruption Phenotype Enhanced apoptotic-like cell death late in development. {ECO:0000269|PubMed:14563678}.
Function Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Possesses high activity on ceramide analogs (C6, C8 synthetic ceramides) and lower activity on C6 and C8 dihydroceramides. Has weak activity on natural ceramides (a mixture of ceramides from bovine brain) and the synthetic substrate C2 ceramide. Has very poor activity on diacylglycerol and sphingosine. Ceramide is a critical sphingolipid metabolite that induces programmed cell death (PCD) in plants and ceramide-1- phosphate has a PCD suppressive effect. Thus, ceramide phosphorylation plays a role in the modulation of PCD and CERK activity is crucial for the maintenance of cell viability. {ECO:0000269|PubMed:14563678}.
Induction By infection with bacterial pathogen P.syringae. {ECO:0000269|PubMed:14563678}.
Sequence Caution Sequence=BAA97392.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Contains 1 DAGKc domain. {ECO:0000255|PROSITE- ProRule:PRU00783}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010021 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
42568457 RefSeq NP_568756 608 ceramide kinase

Identical Sequences to LMP010021 proteins

Reference Database Accession Length Protein Name
GI:42568457 GenBank AAQ62904.1 608 ceramide kinase [Arabidopsis thaliana]
GI:42568457 gnl TAIR 608 ceramide kinase [Arabidopsis thaliana]
GI:42568457 SwissProt Q6USK2.1 608 RecName: Full=Ceramide kinase; Short=AtCERK; AltName: Full=Protein ACCELERATED CELL DEATH 5 [Arabidopsis thaliana]

Related Sequences to LMP010021 proteins

Reference Database Accession Length Protein Name
GI:42568457 DBBJ BAA97392.1 533 unnamed protein product [Arabidopsis thaliana]
GI:42568457 GenBank EOA13057.1 647 hypothetical protein CARUB_v10026058mg, partial [Capsella rubella]
GI:42568457 GenBank KFK26741.1 614 hypothetical protein AALP_AA8G287000 [Arabis alpina]
GI:42568457 RefSeq XP_006280159.1 647 hypothetical protein CARUB_v10026058mg, partial [Capsella rubella]
GI:42568457 RefSeq XP_010442986.1 614 PREDICTED: ceramide kinase [Camelina sativa]
GI:42568457 RefSeq XP_010482463.1 610 PREDICTED: ceramide kinase-like [Camelina sativa]