Gene/Proteome Database (LMPD)

LMPD ID
LMP010066
Gene ID
839470
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
cytochrome P450, family 703, subfamily A, polypeptide 2
Gene Symbol
CYP703A2
Synonyms
CYP703; CYP703A2; cytochrome P450; F6F3.8; F6F3_8; family 703; polypeptide 2; subfamily A
Alternate Names
cytochrome P450, family 703, subfamily A, polypeptide 2
Chromosome
1
EC Number
1.14.-.-
Summary
member of CYP703A CYP703A2 is expressed specifically in anthers of land plants, catalyzing the in-chain hydroxylation at the C-7 position of medium-chain saturated fatty acids (lauric acid in-chain hydroxylase) which is involved in pollen development (sporopollenin synthesis).
Orthologs
View orthologs and multiple alignments for CYP703A2

Proteins

cytochrome P450, family 703, subfamily A, polypeptide 2
Refseq ID NP_171635
Protein GI 15223365
UniProt ID Q9LNJ4
mRNA ID NM_100010
Length 510
RefSeq Status REVIEWED
MILVLASLFAVLILNVLLWRWLKASACKAQRLPPGPPRLPILGNLLQLGPLPHRDLASLCDKYGPLVYLRLGNVDAITTNDPDTIREILLRQDDVFSSRPKTLAAVHLAYGCGDVALAPMGPHWKRMRRICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLGKQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCEKEMRDVEKRVDEFHTKIIDEHRRAKLEDEDKNGDMDFVDVLLSLPGENGKAHMEDVEIKALIQDMIAAATDTSAVTNEWAMAEAIKQPRVMRKIQEELDNVVGSNRMVDESDLVHLNYLRCVVRETFRMHPAGPFLIPHESVRATTINGYYIPAKTRVFINTHGLGRNTKIWDDVEDFRPERHWPVEGSGRVEISHGPDFKILPFSAGKRKCPGAPLGVTMVLMALARLFHCFEWSSPGNIDTVEVYGMTMPKAKPLRAIAKPRLAAHLYT

Gene Information

Entrez Gene ID
839470
Gene Name
cytochrome P450, family 703, subfamily A, polypeptide 2
Gene Symbol
CYP703A2
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0020037 IEA:InterPro F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0016709 IDA:TAIR F oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
GO:0051792 IDA:TAIR P medium-chain fatty acid biosynthetic process
GO:0051791 IDA:TAIR P medium-chain fatty acid metabolic process
GO:0010584 IMP:TAIR P pollen exine formation
GO:0010208 IMP:TAIR P pollen wall assembly
GO:0080110 IMP:TAIR P sporopollenin biosynthetic process

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6468 omega- hydroxylation of laurate
PWY-6733 sporopollenin precursor biosynthesis
PWY-6733 sporopollenin precursor biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR001128 Cytochrome P450
IPR002401 Cytochrome P450, E-class, group I
IPR017972 Cytochrome P450, conserved site

UniProt Annotations

Entry Information

Gene Name
cytochrome P450, family 703, subfamily A, polypeptide 2
Protein Entry
C70A2_ARATH
UniProt ID
Q9LNJ4
Species
Arabidopsis

Comments

Comment Type Description
Cofactor Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
Developmental Stage Specifically expressed in the tapetum cell layer and the microspores of anthers at the tetrad stage. Expression starts to decrease during pollen maturation and tapetum cell degeneration to finally disappear. {ECO:0000269|PubMed:17496121}.
Disruption Phenotype Impaired pollen development and partial male-sterile phenotype. {ECO:0000269|PubMed:17496121}.
Function Involved in pollen wall development. Catalyzes the conversion of medium-chain saturated fatty acids to the corresponding monohydroxylated fatty acids, with a preferential hydroxylation of lauric acid at the C-7 position. In-chain hydroxylated fatty acids, together with omega-hydroxylated fatty acids, are key monomeric aliphatic building blocks for sporopollenin synthesis during exine formation. {ECO:0000269|PubMed:17496121}.
Similarity Belongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular Location Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010066 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15223365 RefSeq NP_171635 510 cytochrome P450, family 703, subfamily A, polypeptide 2

Identical Sequences to LMP010066 proteins

Reference Database Accession Length Protein Name
GI:15223365 GenBank AAF97323.1 510 Putative cytochrome P450 [Arabidopsis thaliana]
GI:15223365 GenBank AAY25435.1 510 At1g01280 [Arabidopsis thaliana]
GI:15223365 GenBank ACX20389.1 510 Sequence 48901 from patent US 7569389
GI:15223365 GenBank AEE27265.1 510 cytochrome P450, family 703, subfamily A, polypeptide 2 [Arabidopsis thaliana]
GI:15223365 GenBank AEL86880.1 510 Sequence 3745 from patent US 7989676
GI:15223365 SwissProt Q9LNJ4.1 510 RecName: Full=Cytochrome P450 703A2 [Arabidopsis thaliana]

Related Sequences to LMP010066 proteins

Reference Database Accession Length Protein Name
GI:15223365 GenBank EOA38917.1 510 hypothetical protein CARUB_v10011315mg [Capsella rubella]
GI:15223365 RefSeq XP_006306019.1 510 hypothetical protein CARUB_v10011315mg [Capsella rubella]
GI:15223365 RefSeq XP_006418407.1 510 hypothetical protein EUTSA_v10007395mg [Eutrema salsugineum]
GI:15223365 RefSeq XP_010501277.1 510 PREDICTED: cytochrome P450 703A2 [Camelina sativa]
GI:15223365 RefSeq XP_010457143.1 510 PREDICTED: cytochrome P450 703A2-like [Camelina sativa]
GI:15223365 RefSeq XP_010474719.1 510 PREDICTED: cytochrome P450 703A2-like [Camelina sativa]