Gene/Proteome Database (LMPD)

LMPD ID
LMP010070
Gene ID
818012
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
cytochrome P450 710A2
Gene Symbol
CYP710A2
Synonyms
cytochrome P450; F13P17.22; family 710; polypeptide 2; subfamily A
Alternate Names
cytochrome P450 710A2
Chromosome
2
EC Number
1.14.-.-
Summary
Encodes a protein with C22-sterol desaturase activity. The enzyme was shown to catalyze the conversion of both 24-<i>epi</i>-campesterol and &beta;-sitosterol to brassicasterol and stigmasterol, respectively, in the presence of NADPH.
Orthologs
View orthologs and multiple alignments for CYP710A2

Proteins

cytochrome P450 710A2
Refseq ID NP_180996
Protein GI 15226756
UniProt ID O64698
mRNA ID NM_129001
Length 499
RefSeq Status REVIEWED
MVFSVSIFASLAPYLVSALLLFFLIEQLSYLVKKRNLPGPLFVPPIIGNAISLVRDPTSFWFKQSDTAGTSPGLAANYLIGKFIIYIRDTELSHQIFSNVRLEAFHPLGHPFGKQLFGDHSLIYLFGEDHKTVRRHLAPNFTPKALSTYSDLQQIVMLRHLRQWEESFSGGTKPVSMRDLVRELNLETSQTVFVGPYLDKEARNTFCTDYNLFNLGSMALPINLPGFAFNKARRAVMNLEKTLSVCAGKSKKRMATGEEPTCLIDFWMHAFVTEIESGNPPPLHSEDEAIGGLLFDFLFAAQDASTSSLLWAVTFLESHPKVLSKVREEVAKIWSPQSGHLITADQLAEMKYTRAVAREVVRYRPPATMVPHIATNDFPLTESYTIPKGTIVFPSVFDASFQGFTEPNRFDPDRFSETRQEDQVFKRNYLAFGWGAHQCVGQRYALNHLVLFIAMFSSLFDFKRLQSDGCDDIIYCPTISPKDGCTVFLSKRIVTYPNL

Gene Information

Entrez Gene ID
818012
Gene Name
cytochrome P450 710A2
Gene Symbol
CYP710A2
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0000249 IDA:TAIR F C-22 sterol desaturase activity
GO:0020037 IEA:InterPro F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0004497 IEA:UniProtKB-KW F monooxygenase activity
GO:0016126 IEA:UniProtKB-UniPathway P sterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01110 Biosynthesis of secondary metabolites
ath00100 Steroid biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-2541 plant sterol biosynthesis
PWY-2541 plant sterol biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR001128 Cytochrome P450
IPR002401 Cytochrome P450, E-class, group I
IPR017972 Cytochrome P450, conserved site

UniProt Annotations

Entry Information

Gene Name
cytochrome P450 710A2
Protein Entry
C7102_ARATH
UniProt ID
O64698
Species
Arabidopsis

Comments

Comment Type Description
Cofactor Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
Disruption Phenotype No visible phenotype under normal growth conditions, but production of brassicasterol is abolished. {ECO:0000269|PubMed:16531502}.
Function Required to form the C-22 double bond in the sterol side chain. Possesses in vitro C-22 desaturase activity toward 24-epi- campesterol and beta-sitosterol and produces brassicasterol and stigmasterol, respectively. {ECO:0000269|PubMed:16531502}.
Miscellaneous Plants overexpressing CYP710A2 show higher levels of brassicasterol/crinosterol and stigmasterol, and lower levels of 24-epi-campesterol/campesterol and beta-sitosterol than the wild-type.
Pathway Steroid biosynthesis; sterol biosynthesis.
Similarity Belongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular Location Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Tissue Specificity Expressed in the vascular tissues of roots, shoots, stems and leaves. Expressed in root tips, carpes, siliques and seeds. {ECO:0000269|PubMed:16531502}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010070 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15226756 RefSeq NP_180996 499 cytochrome P450 710A2

Identical Sequences to LMP010070 proteins

Reference Database Accession Length Protein Name
GI:15226756 DBBJ BAE80316.1 499 CYP710 protein [Arabidopsis thaliana]
GI:15226756 GenBank ACW84715.1 499 Sequence 407 from patent US 7569389
GI:15226756 GenBank ACX16856.1 499 Sequence 44129 from patent US 7569389
GI:15226756 GenBank ACX21764.1 499 Sequence 50767 from patent US 7569389
GI:15226756 GenBank AEC08981.1 499 cytochrome P450 710A2 [Arabidopsis thaliana]
GI:15226756 GenBank AEL87028.1 499 Sequence 3984 from patent US 7989676

Related Sequences to LMP010070 proteins

Reference Database Accession Length Protein Name
GI:15226756 EMBL CBF74464.1 499 unnamed protein product [Arabidopsis thaliana]
GI:15226756 EMBL CBU96667.1 499 unnamed protein product [Arabidopsis thaliana]
GI:15226756 GenBank AAM62799.1 499 putative cytochrome P450 [Arabidopsis thaliana]
GI:15226756 GenBank EFH57597.1 499 CYP710A2 [Arabidopsis lyrata subsp. lyrata]
GI:15226756 GenBank AGX64095.1 499 Sequence 28294 from patent US 8541208
GI:15226756 RefSeq XP_002881338.1 499 CYP710A2 [Arabidopsis lyrata subsp. lyrata]