Gene/Proteome Database (LMPD)

LMPD ID
LMP010111
Gene ID
814686
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
DELLA protein RGA
Gene Symbol
RGA1
Synonyms
F2I9.19; F2I9_19; REPRESSOR OF GA; REPRESSOR OF GA1-3 1; RGA; RGA1
Alternate Names
DELLA protein RGA
Chromosome
2
Summary
Member of the VHIID/DELLA regulatory family. Contains homopolymeric serine and threonine residues, a putative nuclear localization signal, leucine heptad repeats, and an LXXLL motif. Putative transcriptional regulator repressing the gibberellin response and integration of phytohormone signalling. DELLAs repress cell proliferation and expansion that drives plant growth. The protein undergoes degradation in response to GA via the 26S proteasome. RGA1 binds to PIF3 and inhibits its DNA binding activity and thus affects the expression of PIF3 regulated genes. RGA may be involved in reducing ROS accumulation in response to stress by up-regulating the transcription of superoxide dismutases. Represses GA-induced vegetative growth and floral initiation. Rapidly degraded in response to GA. Involved in fruit and flower development.
Orthologs
View orthologs and multiple alignments for RGA1

Proteins

DELLA protein RGA
Refseq ID NP_178266
Protein GI 15226311
UniProt ID Q9SLH3
mRNA ID NM_126218
Length 587
RefSeq Status REVIEWED
MKRDHHQFQGRLSNHGTSSSSSSISKDKMMMVKKEEDGGGNMDDELLAVLGYKVRSSEMAEVALKLEQLETMMSNVQEDGLSHLATDTVHYNPSELYSWLDNMLSELNPPPLPASSNGLDPVLPSPEICGFPASDYDLKVIPGNAIYQFPAIDSSSSSNNQNKRLKSCSSPDSMVTSTSTGTQIGGVIGTTVTTTTTTTTAAGESTRSVILVDSQENGVRLVHALMACAEAIQQNNLTLAEALVKQIGCLAVSQAGAMRKVATYFAEALARRIYRLSPPQNQIDHCLSDTLQMHFYETCPYLKFAHFTANQAILEAFEGKKRVHVIDFSMNQGLQWPALMQALALREGGPPTFRLTGIGPPAPDNSDHLHEVGCKLAQLAEAIHVEFEYRGFVANSLADLDASMLELRPSDTEAVAVNSVFELHKLLGRPGGIEKVLGVVKQIKPVIFTVVEQESNHNGPVFLDRFTESLHYYSTLFDSLEGVPNSQDKVMSEVYLGKQICNLVACEGPDRVERHETLSQWGNRFGSSGLAPAHLGSNAFKQASMLLSVFNSGQGYRVEESNGCLMLGWHTRPLITTSAWKLSTAAY

Gene Information

Entrez Gene ID
814686
Gene Name
DELLA protein RGA
Gene Symbol
RGA1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005634 IDA:TAIR C nucleus
GO:0003700 IDA:TAIR F sequence-specific DNA binding transcription factor activity
GO:0044212 IPI:TAIR F transcription regulatory region DNA binding
GO:0009740 TAS:TAIR P gibberellic acid mediated signaling pathway
GO:0042538 IGI:TAIR P hyperosmotic salinity response
GO:0009867 IGI:TAIR P jasmonic acid mediated signaling pathway
GO:0007275 IEA:UniProtKB-KW P multicellular organismal development
GO:0009938 IGI:TAIR P negative regulation of gibberellic acid mediated signaling pathway
GO:0010187 IGI:TAIR P negative regulation of seed germination
GO:0042176 IMP:TAIR P regulation of protein catabolic process
GO:2000377 IGI:TAIR P regulation of reactive oxygen species metabolic process
GO:2000033 IGI:TAIR P regulation of seed dormancy process
GO:0010029 IGI:TAIR P regulation of seed germination
GO:0006355 TAS:TAIR P regulation of transcription, DNA-templated
GO:0009737 IGI:TAIR P response to abscisic acid
GO:0009723 IGI:TAIR P response to ethylene
GO:0010218 IEP:TAIR P response to far red light
GO:0009651 IGI:TAIR P response to salt stress
GO:0006351 IEA:UniProtKB-KW P transcription, DNA-templated

KEGG Pathway Links

KEGG Pathway ID Description
ath04075 Plant hormone signal transduction
ko04075 Plant hormone signal transduction

Domain Information

InterPro Annotations

Accession Description
IPR005202 Transcription factor GRAS
IPR021914 Transcriptional factor DELLA, N-terminal

UniProt Annotations

Entry Information

Gene Name
DELLA protein RGA
Protein Entry
RGA_ARATH
UniProt ID
Q9SLH3
Species
Arabidopsis

Comments

Comment Type Description
Disruption Phenotype Rga, gai, rgl1, rgl2 and rgl3 pentuple mutant displays constitutive GA responses even in the absence of GA treatment. {ECO:0000269|PubMed:23482857}.
Domain The DELLA motif is required for its GA-induced degradation but not for the interaction with GID2. {ECO:0000269|PubMed:11717468}.
Function Probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Positively regulates XERICO expression in seeds. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. Compared to other DELLA proteins, it is the most sensitive to GA application. No effect of the BOI proteins on its stability. Its activity is probably regulated by other phytohormones such as auxin and ethylene, attenuation of auxin transport delaying its GA-induced degradation. {ECO:0000269|PubMed:11606551, ECO:0000269|PubMed:11606552, ECO:0000269|PubMed:12610625, ECO:0000269|PubMed:14615596, ECO:0000269|PubMed:14973286, ECO:0000269|PubMed:15128937, ECO:0000269|PubMed:16034591, ECO:0000269|PubMed:17933900, ECO:0000269|PubMed:20093430, ECO:0000269|PubMed:9490740}.
Interaction Q9MAA7:GID1A; NbExp=7; IntAct=EBI-963624, EBI-963597; Q9LYC1:GID1B; NbExp=6; IntAct=EBI-963624, EBI-963686; Q940G6:GID1C; NbExp=5; IntAct=EBI-963624, EBI-963794; Q9STX3:GID2; NbExp=4; IntAct=EBI-963624, EBI-619033;
Ptm Phosphorylated. Phosphorylation may increase the interaction with GID2. {ECO:0000269|PubMed:15161962}.
Ptm Ubiquitinated. Upon GA application it is ubiquitinated by the SCF(GID2) complex, leading to its subsequent degradation.
Similarity Belongs to the GRAS family. DELLA subfamily. {ECO:0000305}.
Subcellular Location Nucleus {ECO:0000269|PubMed:20093430, ECO:0000269|PubMed:9490740}.
Subunit Interacts directly with the GID2/SLY1 component of the SCF(GID2) complex. Interacts (via N-terminus) with GID1A, GID1B and GID1B (via N-terminus). Binds to bHLH transcription factors such as MYC2, PIF1, PIF4, PIF6 and SPT. Interacts with the BOI proteins BOI, BRG1, BRG2 and BRG3. {ECO:0000269|PubMed:15155881, ECO:0000269|PubMed:15161962, ECO:0000269|PubMed:15173565, ECO:0000269|PubMed:16709201, ECO:0000269|PubMed:20093430, ECO:0000269|PubMed:22669881, ECO:0000269|PubMed:23482857}.
Tissue Specificity Ubiquitously expressed. Expressed in roots, rosette leaves, bolting and mature stems, young and mature siliques, flower buds and influorescences. {ECO:0000269|PubMed:11877383, ECO:0000269|PubMed:20093430, ECO:0000269|PubMed:9237632, ECO:0000269|PubMed:9490740}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010111 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15226311 RefSeq NP_178266 587 DELLA protein RGA

Identical Sequences to LMP010111 proteins

Reference Database Accession Length Protein Name
GI:15226311 GenBank AAL06821.1 587 At2g01570/F2I9.19 [Arabidopsis thaliana]
GI:15226311 GenBank AAQ65090.1 587 At2g01570/F2I9.19 [Arabidopsis thaliana]
GI:15226311 GenBank AEC05469.1 587 DELLA protein RGA [Arabidopsis thaliana]
GI:15226311 gnl TIGR 587 putative RGA1, giberellin repsonse modulation protein [Arabidopsis thaliana]
GI:15226311 SwissProt Q9SLH3.1 587 RecName: Full=DELLA protein RGA; AltName: Full=GAI-related sequence; AltName: Full=GRAS family protein 10; Short=AtGRAS-10; AltName: Full=Repressor on the ga1-3 mutant; AltName: Full=Restoration of growth on ammonia protein 1 [Arabidopsis thaliana]

Related Sequences to LMP010111 proteins

Reference Database Accession Length Protein Name
GI:15226311 EMBL CAA72177.1 587 RGA1 protein [Arabidopsis thaliana]
GI:15226311 EMBL CAJ29245.1 587 unnamed protein product [Arabidopsis thaliana]
GI:15226311 GenBank AAK97709.1 587 At2g01570/F2I9.19 [Arabidopsis thaliana]
GI:15226311 GenBank ACC28661.1 587 Sequence 216 from patent US 7345217
GI:15226311 GenBank ACX05991.1 587 Sequence 29376 from patent US 7569389
GI:15226311 GenBank AEQ40092.1 587 Sequence 262 from patent US 8030546