Gene/Proteome Database (LMPD)

LMPD ID
LMP010135
Gene ID
816464
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
diacylglycerol O-acyltransferase 1
Gene Symbol
TAG1
Synonyms
ABX45; AS11; ATDGAT; DGAT1; DIACYLGLYCEROL ACYLTRANSFERASE; F27F23.24; RDS1; TAG1; TRIACYLGLYCEROL BIOSYNTHESIS DEFECT 1
Alternate Names
diacylglycerol O-acyltransferase 1
Chromosome
2
EC Number
2.3.1.20
Summary
Encodes Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the final step of the triacylglycerol synthesis pathway. An insertion mutation in the TAG1 gene results in altered lipid phenotype. Role in senescence and seed development.
Orthologs
View orthologs and multiple alignments for TAG1

Proteins

diacylglycerol O-acyltransferase 1
Refseq ID NP_179535
Protein GI 15224779
UniProt ID Q9SLD2
mRNA ID NM_127503
Length 520
RefSeq Status REVIEWED
MAILDSAGVTTVTENGGGEFVDLDRLRRRKSRSDSSNGLLLSGSDNNSPSDDVGAPADVRDRIDSVVNDDAQGTANLAGDNNGGGDNNGGGRGGGEGRGNADATFTYRPSVPAHRRARESPLSSDAIFKQSHAGLFNLCVVVLIAVNSRLIIENLMKYGWLIRTDFWFSSRSLRDWPLFMCCISLSIFPLAAFTVEKLVLQKYISEPVVIFLHIIITMTEVLYPVYVTLRCDSAFLSGVTLMLLTCIVWLKLVSYAHTSYDIRSLANAADKANPEVSYYVSLKSLAYFMVAPTLCYQPSYPRSACIRKGWVARQFAKLVIFTGFMGFIIEQYINPIVRNSKHPLKGDLLYAIERVLKLSVPNLYVWLCMFYCFFHLWLNILAELLCFGDREFYKDWWNAKSVGDYWRMWNMPVHKWMVRHIYFPCLRSKIPKTLAIIIAFLVSAVFHELCIAVPCRLFKLWAFLGIMFQVPLVFITNYLQERFGSTVGNMIFWFIFCIFGQPMCVLLYYHDLMNRKGSMS

Gene Information

Entrez Gene ID
816464
Gene Name
diacylglycerol O-acyltransferase 1
Gene Symbol
TAG1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009941 IDA:TAIR C chloroplast envelope
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:TAIR C membrane
GO:0004144 IDA:TAIR F diacylglycerol O-acyltransferase activity
GO:0007568 IEP:TAIR P aging
GO:0005975 IMP:TAIR P carbohydrate metabolic process
GO:0009793 TAS:TAIR P embryo development ending in seed dormancy
GO:0006071 IEA:UniProtKB-KW P glycerol metabolic process
GO:0010030 IMP:TAIR P positive regulation of seed germination
GO:0045995 IMP:TAIR P regulation of embryonic development
GO:0010029 IMP:TAIR P regulation of seed germination
GO:0009737 IMP:TAIR P response to abscisic acid
GO:0009409 IMP:TAIR P response to cold
GO:0009749 IMP:TAIR P response to glucose
GO:0009651 IMP:TAIR P response to salt stress
GO:0019432 IDA:TAIR P triglyceride biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath00561 Glycerolipid metabolism
ath01100 Metabolic pathways

REACTOME Pathway Links

REACTOME Pathway ID Description
6254309 Acyl chain remodeling of DAG and TAG

Domain Information

InterPro Annotations

Accession Description
IPR027251 Diacylglycerol O-acyltransferase 1
IPR004299 Membrane bound O-acyl transferase, MBOAT
IPR014371 Sterol O-acyltransferase, ACAT/DAG/ARE types

UniProt Annotations

Entry Information

Gene Name
diacylglycerol O-acyltransferase 1
Protein Entry
DGAT1_ARATH
UniProt ID
Q9SLD2
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties pH dependence: Optimum pH is between 6.75-7.25. {ECO:0000269|PubMed:11171171};
Catalytic Activity Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol. {ECO:0000269|PubMed:11171171}.
Developmental Stage Peak of expression in seeds 15 days after flowering. Highly expressed in senescing leaves. {ECO:0000269|PubMed:12177474, ECO:0000269|PubMed:20101470}.
Disruption Phenotype Decreased oil content and modified size and shape of oil bodies. {ECO:0000269|PubMed:20040537}.
Enzyme Regulation Partially inhibited by niacin. {ECO:0000269|PubMed:11171171}.
Function Major enzyme for oil accumulation in seeds. Catalyzes the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. Can use palmitoyl-CoA and oleoyl-CoA as substrates. Has complementary functions with PDAT1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen. {ECO:0000269|PubMed:10580283, ECO:0000269|PubMed:10601854, ECO:0000269|PubMed:12114588, ECO:0000269|PubMed:20040537, ECO:0000269|PubMed:20101470}.
Induction Up-regulated by glucose. {ECO:0000269|PubMed:12825687}.
Miscellaneous DGAT1 deficiency alters carbohydrate metabolism.
Miscellaneous The AS11 mutant has a complete duplication of exon 2.
Pathway Glycerolipid metabolism; triacylglycerol biosynthesis.
Similarity Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. {ECO:0000305}.
Subcellular Location Plastid, chloroplast membrane {ECO:0000269|PubMed:12177474}; Multi-pass membrane protein {ECO:0000269|PubMed:12177474}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:12177474}; Multi-pass membrane protein {ECO:0000269|PubMed:12177474}.
Tissue Specificity Ubiquitous. Highest expression in young developing seeds. {ECO:0000269|PubMed:10571850, ECO:0000269|PubMed:12825687, ECO:0000269|PubMed:20040537, ECO:0000269|PubMed:20101470}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010135 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15224779 RefSeq NP_179535 520 diacylglycerol O-acyltransferase 1

Identical Sequences to LMP010135 proteins

Reference Database Accession Length Protein Name
GI:15224779 GenBank AFA83945.1 520 Sequence 17 from patent US 8101819
GI:15224779 GenBank AFA84053.1 520 Sequence 219 from patent US 8101819
GI:15224779 GenBank AFD40685.1 520 Sequence 39 from patent US 8124835
GI:15224779 GenBank AGN61918.1 520 Sequence 17 from patent US 8455714
GI:15224779 GenBank AGN62026.1 520 Sequence 219 from patent US 8455714
GI:15224779 GenBank AGU30561.1 520 Sequence 26 from patent US 8497362

Related Sequences to LMP010135 proteins

Reference Database Accession Length Protein Name
GI:15224779 GenBank ABE24132.1 547 Sequence 25 from patent US 7015373
GI:15224779 GenBank ACC37859.1 547 Sequence 25 from patent US 7355097
GI:15224779 GenBank ACQ20589.1 547 Sequence 25 from patent US 7511189
GI:15224779 GenBank EFH62298.1 510 hypothetical protein ARALYDRAFT_480528 [Arabidopsis lyrata subsp. lyrata]
GI:15224779 GenBank AFA84034.1 547 Sequence 200 from patent US 8101819
GI:15224779 GenBank AGN62007.1 547 Sequence 200 from patent US 8455714