Gene/Proteome Database (LMPD)

LMPD ID
LMP010161
Gene ID
840312
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Gene Symbol
STT3B
Synonyms
F12G12.5; F12G12_5; staurosporin and temperature sensitive 3-like b; STT3B
Alternate Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Chromosome
1
EC Number
2.4.99.18
Summary
Encodes homolog of yeast STT3, a subunit of oligosaccharyltransferase.
Orthologs
View orthologs and multiple alignments for STT3B

Proteins

dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Refseq ID NP_174675
Protein GI 30693010
UniProt ID Q9FX21
mRNA ID NM_103136
Length 735
RefSeq Status REVIEWED
MGGKSEPAKSESMATKPDLLNTSFFSFKSLKLKTKQQELLLRISILGLVYILAFIARLFSVLRYESMIHEFDPYFNYRTTLFLTEKGFYEFWNWFDSESWYPLGRIIGGTLYPGLMVTAALIYWTLRFLRFFVHIREVCVLTAPFFASNTTLVAYFFGKELWDTGAGLVAAVLIAICPGYISRSVAGSYDNEAVAIFALLLTFYLFVKAVNTGSLAWALASAFGYFYMVSAWGGYVFIINLVPLYVLVLLITGRYSMRLYIAYNCMYILGMLLAMQIRFVGFQHVQSGEHMGAMGVFLLMQVFYFLDWVKYQLNDTKLFQTFLRITVTSAILVGGVAVGVGTASGYISPWTGRFYSLLDPTYAKDHIPIIASVSEHQPTAWSSFMFDYHILLFLFPAGLYFCFKRLTDATIFIVMYGLTSLYFAGVMVRLILVATPAVCLISAIAVSATIKNLTSLLRTKQKVSQTGSTKGAGSSKASSKVTLDQSQPFQKNGAIALLVGVFYLLSRYAIHCTWVTAEAYSSPSIVLAARGAHGNRIIFDDYREAYYWLRQNTATDAKIMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDDAYDIMRSLDVNYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEFRVDKGASPKMLNCLMYKLCYYRFGELTTEYGKPPGYDRARGVEIGNKDIKLEHLEEAYTTSNWIVRIYRVKPPTNRL

Gene Information

Entrez Gene ID
840312
Gene Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Gene Symbol
STT3B
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:TAIR C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 IDA:TAIR C membrane
GO:0005886 IDA:TAIR C plasma membrane
GO:0004576 IEA:InterPro F oligosaccharyl transferase activity
GO:0006486 IEA:UniProtKB-UniPathway P protein glycosylation

KEGG Pathway Links

KEGG Pathway ID Description
ath00510 N-Glycan biosynthesis
ath_M00072 N-glycosylation by oligosaccharyltransferase
ath04141 Protein processing in endoplasmic reticulum
ko04141 Protein processing in endoplasmic reticulum

Domain Information

InterPro Annotations

Accession Description
IPR003674 Oligosaccharyl transferase, STT3 subunit

UniProt Annotations

Entry Information

Gene Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Protein Entry
STT3B_ARATH
UniProt ID
Q9FX21
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
Disruption Phenotype No visible phenotype. {ECO:0000269|PubMed:12972670}.
Function Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity (By similarity). {ECO:0000250}.
Pathway Protein modification; protein glycosylation.
Sequence Caution Sequence=BX816490; Type=Frameshift; Positions=280, 283, 286; Evidence={ECO:0000305};
Similarity Belongs to the STT3 family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:16618929, ECO:0000269|PubMed:22923678}; Multi- pass membrane protein {ECO:0000269|PubMed:16618929, ECO:0000269|PubMed:22923678}.
Subunit Component of the oligosaccharyltransferase (OST) complex. {ECO:0000250}.
Tissue Specificity Expressed preferentially in the root but also in the shoot. {ECO:0000269|PubMed:12972670}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010161 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
30693010 RefSeq NP_174675 735 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B

Identical Sequences to LMP010161 proteins

Reference Database Accession Length Protein Name
GI:30693010 GenBank AAG12524.1 735 Putative integral membrane protein [Arabidopsis thaliana]
GI:30693010 GenBank AEE31675.1 735 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [Arabidopsis thaliana]
GI:30693010 GenBank AHL38912.1 735 glycosyltransferase, partial [Arabidopsis thaliana]
GI:30693010 SwissProt Q9FX21.1 735 RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B; Short=Oligosaccharyl transferase subunit STT3B; Short=STT3-B; AltName: Full=Protein STAUROSPORIN AND TEMPERATURE SENSITIVE 3-LIKE B [Arabidopsis thaliana]

Related Sequences to LMP010161 proteins

Reference Database Accession Length Protein Name
GI:30693010 GenBank EFH67337.1 738 hypothetical protein ARALYDRAFT_891007 [Arabidopsis lyrata subsp. lyrata]
GI:30693010 GenBank EOA39754.1 739 hypothetical protein CARUB_v10008402mg [Capsella rubella]
GI:30693010 RefSeq XP_002891078.1 738 hypothetical protein ARALYDRAFT_891007 [Arabidopsis lyrata subsp. lyrata]
GI:30693010 RefSeq XP_006306856.1 739 hypothetical protein CARUB_v10008402mg [Capsella rubella]
GI:30693010 RefSeq XP_010499953.1 738 PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B-like [Camelina sativa]
GI:30693010 RefSeq XP_010478833.1 738 PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B isoform X2 [Camelina sativa]