Gene/Proteome Database (LMPD)

LMPD ID
LMP010162
Gene ID
819388
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
Gene Symbol
ALG3
Synonyms
Arabidopsis thaliana asparagine-linked glycosylation 3; asparagine-linked glycosylation 3; AtALG3; F17A22.15
Alternate Names
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
Chromosome
2
EC Number
2.4.1.258

Proteins

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
Refseq ID NP_001031556
Protein GI 79324943
UniProt ID O82244
mRNA ID NM_001036479
Length 437
RefSeq Status REVIEWED
MAGASSPASLRASRSRRLGKETNRSDLFKKPAVPFAFALILADAILVALIIAYVPYTKIDWDAYMSQVSGFLGGERDYGNLKGDTGPLVYPAGFLYVYSAVQNLTGGEVYPAQILFGVLYIVNLGIVLIIYVKTDVPWWALSLLCLSKRIHSIFVLRLFNDCFAMTLLHASMALFLYRKWHLGMLVFSGAVSVKMNVLLYAPTLLLLLLKAMNIIGVVSALAGAALVQILVGLPFLITYPVSYIANAFDLGRVFIHFWSVNFKFVPERVFVSKEFAVCLLIAHLFLLVAFANYKWCKHEGGIIGFMRSRHFFLTLPSSLSFSDVSASRIITKEHVVTAMFVGNFIGIVFARSLHYQFYSWYFYSLPYLLWRTPFPTWLRLIMFLGIELCWNVYPSTPSSSGLLLCLHLIILVGLWLAPSVDPYQLKEHPKSQIHKKA
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
Refseq ID NP_001031557
Protein GI 79324945
UniProt ID O82244
mRNA ID NM_001036480
Length 380
RefSeq Status REVIEWED
MAGASSPASLRASRSRRLGKETNRSDLFKKPAVPFAFALILADAILVALIIAYVPYTKIDWDAYMSQVSGFLGGERDYGNLKGDTGPLVYPAGFLYVYSAVQNLTGGEVYPAQILFGVLYIVNLGIVLIIYVKTDVVPWWALSLLCLSKRIHSIFVLRLFNDCFAMTLLHASMALFLYRKWHLGMLVFSGAVSVKMNVLLYAPTLLLLLLKAMNIIGVVSALAGAALVQILVGLPFLITYPVSYIANAFDLGRVFIHFWSVNFKFVPERVFVSKEFAVCLLIAHLFLLVAFANYKWCKHEGGIIGFMRSRHFFLTLPSSLSFSDVSASRIITKEHVVTAMFVGNFIGIVFARSLHYQFYSWYFYSLPYLLWRTPFPTWLR
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
Refseq ID NP_182297
Protein GI 15227129
UniProt ID O82244
mRNA ID NM_130343
Length 438
RefSeq Status REVIEWED
MAGASSPASLRASRSRRLGKETNRSDLFKKPAVPFAFALILADAILVALIIAYVPYTKIDWDAYMSQVSGFLGGERDYGNLKGDTGPLVYPAGFLYVYSAVQNLTGGEVYPAQILFGVLYIVNLGIVLIIYVKTDVVPWWALSLLCLSKRIHSIFVLRLFNDCFAMTLLHASMALFLYRKWHLGMLVFSGAVSVKMNVLLYAPTLLLLLLKAMNIIGVVSALAGAALVQILVGLPFLITYPVSYIANAFDLGRVFIHFWSVNFKFVPERVFVSKEFAVCLLIAHLFLLVAFANYKWCKHEGGIIGFMRSRHFFLTLPSSLSFSDVSASRIITKEHVVTAMFVGNFIGIVFARSLHYQFYSWYFYSLPYLLWRTPFPTWLRLIMFLGIELCWNVYPSTPSSSGLLLCLHLIILVGLWLAPSVDPYQLKEHPKSQIHKKA

Gene Information

Entrez Gene ID
819388
Gene Name
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
Gene Symbol
ALG3
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:TAIR C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0000033 IDA:TAIR F alpha-1,3-mannosyltransferase activity
GO:0052925 IEA:UniProtKB-EC F dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity
GO:0097502 IDA:GOC P mannosylation
GO:0006486 IMP:TAIR P protein glycosylation

KEGG Pathway Links

KEGG Pathway ID Description
ath01100 Metabolic pathways
ath00510 N-Glycan biosynthesis
ko00510 N-Glycan biosynthesis
ath_M00055 N-glycan precursor biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR007873 Glycosyltransferase, ALG3

UniProt Annotations

Entry Information

Gene Name
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
Protein Entry
ALG3_ARATH
UniProt ID
O82244
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=O82244-1; Sequence=Displayed; Name=2; IsoId=O82244-2; Sequence=VSP_041719; Name=3; IsoId=O82244-3; Sequence=VSP_041720;
Catalytic Activity Dolichyl beta-D-mannosyl phosphate + D-Man- alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha- (1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc- diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man- alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta- (1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. {ECO:0000269|PubMed:18567790}.
Disruption Phenotype No obvious phenotype under normal and high temperature or salt/osmotic stress conditions. {ECO:0000269|PubMed:20356820}.
Function Required for N-linked oligosaccharide assembly. Adds the sixth mannose residue in an alpha-1,3 linkage onto the dolichol- PP-oligosaccharide precursor dolichol-PP-Man(5)GlcNAc(2). {ECO:0000269|PubMed:18567790, ECO:0000269|PubMed:20356820}.
Miscellaneous In the absence of ALG3 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant.
Pathway Protein modification; protein glycosylation.
Similarity Belongs to the glycosyltransferase 58 family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:18567790, ECO:0000269|PubMed:20356820}; Multi- pass membrane protein {ECO:0000269|PubMed:18567790, ECO:0000269|PubMed:20356820}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010162 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15227129 RefSeq NP_182297 438 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
79324943 RefSeq NP_001031556 437 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
79324945 RefSeq NP_001031557 380 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

Identical Sequences to LMP010162 proteins

Reference Database Accession Length Protein Name
GI:15227129 EMBL CBN61860.1 438 unnamed protein product [Arabidopsis thaliana]
GI:15227129 GenBank AEC10883.1 438 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [Arabidopsis thaliana]
GI:79324943 GenBank AEC10884.1 437 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [Arabidopsis thaliana]
GI:79324945 GenBank AEC10885.1 380 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [Arabidopsis thaliana]
GI:15227129 GenBank AHL38790.1 438 glycosyltransferase, partial [Arabidopsis thaliana]
GI:15227129 gnl TIGR 438 Not56-like protein [Arabidopsis thaliana]
GI:15227129 SwissProt O82244.1 438 RecName: Full=Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase; AltName: Full=Alpha-1,3-mannosyltransferase ALG3; AltName: Full=Asparagine-linked glycosylation protein 3; Short=AtALG3; AltName: Full=Not56-like protein [Arabidopsis thaliana]

Related Sequences to LMP010162 proteins

Reference Database Accession Length Protein Name
GI:79324943 EMBL CBN61860.1 438 unnamed protein product [Arabidopsis thaliana]
GI:79324945 EMBL CBN61860.1 438 unnamed protein product [Arabidopsis thaliana]
GI:15227129 GenBank AAL16193.1 438 At2g47760/F17A22.15 [Arabidopsis thaliana]
GI:15227129 GenBank AAP37662.1 438 At2g47760/F17A22.15 [Arabidopsis thaliana]
GI:15227129 GenBank EFH58390.1 439 At2g47760/F17A22.15 [Arabidopsis lyrata subsp. lyrata]
GI:79324945 GenBank AEC10883.1 438 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [Arabidopsis thaliana]
GI:79324943 GenBank AEC10883.1 438 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [Arabidopsis thaliana]
GI:15227129 GenBank AEC10884.1 437 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [Arabidopsis thaliana]
GI:79324945 GenBank AHL38790.1 438 glycosyltransferase, partial [Arabidopsis thaliana]
GI:79324943 GenBank AHL38790.1 438 glycosyltransferase, partial [Arabidopsis thaliana]
GI:79324945 gnl TIGR 438 Not56-like protein [Arabidopsis thaliana]
GI:79324943 gnl TIGR 438 Not56-like protein [Arabidopsis thaliana]
GI:79324945 RefSeq NP_182297.1 438 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [Arabidopsis thaliana]
GI:79324943 RefSeq NP_182297.1 438 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [Arabidopsis thaliana]
GI:15227129 RefSeq NP_001031556.1 437 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [Arabidopsis thaliana]
GI:15227129 RefSeq XP_002882131.1 439 At2g47760/F17A22.15 [Arabidopsis lyrata subsp. lyrata]
GI:79324943 SwissProt O82244.1 438 RecName: Full=Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase; AltName: Full=Alpha-1,3-mannosyltransferase ALG3; AltName: Full=Asparagine-linked glycosylation protein 3; Short=AtALG3; AltName: Full=Not56-like protein [Arabidopsis thaliana]
GI:79324945 SwissProt O82244.1 438 RecName: Full=Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase; AltName: Full=Alpha-1,3-mannosyltransferase ALG3; AltName: Full=Asparagine-linked glycosylation protein 3; Short=AtALG3; AltName: Full=Not56-like protein [Arabidopsis thaliana]