Gene/Proteome Database (LMPD)

LMPD ID
LMP010172
Gene ID
815152
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
enoyl-[acyl-carrier-protein] reductase [NADH]
Gene Symbol
MOD1
Synonyms
ENOYL-ACP REDUCTASE; ENOYL-ACP REDUCTASE 1; ENR1; MOD1; MOSAIC DEATH 1; T6P5.19; T6P5_19
Alternate Names
enoyl-[acyl-carrier-protein] reductase [NADH]
Chromosome
2
EC Number
1.3.1.9
Summary
Encodes enoyl-ACP reductase a component of the fatty acid synthase complex. A reduced function mutation in this gene, mod1, was found in a screen for premature cell death mutants. Mutant plants have reduced lipid level and pleiotropic morphological defects, including chlorotic and abnormally shaped leaves.
Orthologs
View orthologs and multiple alignments for MOD1

Proteins

enoyl-[acyl-carrier-protein] reductase [NADH]
Refseq ID NP_565331
Protein GI 18396215
UniProt ID Q9SLA8
mRNA ID NM_126612
Length 390
RefSeq Status REVIEWED
MAATAASSLQIATRRPSMSSPSKILKAGTYIVGANPGNASWDKLSCTRQLSNLGCLRNHSAVPTCKRPFSFSTRAMSESSENKAPSGLPIDLRGKRAFIAGIADDNGYGWAIAKSLAAAGAEILVGTWVPALNIFETSLRRGKFDQSRVLPDGSLMEIKKVYALDAVFDNPEDVPEDVKTNKRYAGSSNWTVQEAAECVKKDFGSIDILVHSLANGPEVSKPLLETSRKGYLAAISASSYSFVSLLRHFLPIMNPGGASISLTYIASERIIPGYGGGMSSAKAALESDTRVLAYEAGRKSNIRVNTISAGPLGSRAAKAIGFIDTMIEYSYNNGPIQKTLTADEVGNAAAFLASPLASAITGATIYVDNGLNAMGVALDSPVFKDLNSKN
enoyl-[acyl-carrier-protein] reductase [NADH]
Refseq ID NP_849940
Protein GI 30678246
UniProt ID Q9SLA8
mRNA ID NM_179609
Length 390
RefSeq Status REVIEWED
Protein sequence is identical to GI:18396215 (mRNA isoform)

Gene Information

Entrez Gene ID
815152
Gene Name
enoyl-[acyl-carrier-protein] reductase [NADH]
Gene Symbol
MOD1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009507 IDA:TAIR C chloroplast
GO:0009941 IDA:TAIR C chloroplast envelope
GO:0009570 IDA:TAIR C chloroplast stroma
GO:0005835 TAS:TAIR C fatty acid synthase complex
GO:0009579 IDA:TAIR C thylakoid
GO:0005507 IDA:TAIR F copper ion binding
GO:0016631 IMP:TAIR F enoyl-[acyl-carrier-protein] reductase activity
GO:0004318 IEA:UniProtKB-EC F enoyl-[acyl-carrier-protein] reductase (NADH) activity
GO:0006633 IMP:TAIR P fatty acid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath00780 Biotin metabolism
ath00061 Fatty acid biosynthesis
ath_M00083 Fatty acid biosynthesis, elongation
ath01212 Fatty acid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR002347 Glucose/ribitol dehydrogenase
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
enoyl-[acyl-carrier-protein] reductase [NADH]
Protein Entry
FABI_ARATH
UniProt ID
Q9SLA8
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=288 uM for crotonyl-CoA {ECO:0000269|PubMed:18467464};
Catalytic Activity An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.
Disruption Phenotype Premature cell death in several organs, chlorotic and curly leaves, semidwarfism, distorted siliques, premature senescence of primary inflorescences, reduced fertility and decrease in total lipid content. {ECO:0000269|PubMed:10715326}.
Enzyme Regulation Inhibited by the phytotoxin cyperin and the synthetic antimicrobial compound triclosan. {ECO:0000269|PubMed:18467464}.
Function Catalyzes the NAD-dependent reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Catalyzes the last reduction step in the de novo synthesis cycle of fatty acids. Involved in the elongation cycle of fatty acids which are used in lipid metabolism. Required for normal plant growth. {ECO:0000269|PubMed:10715326, ECO:0000269|PubMed:18467464}.
Pathway Lipid metabolism; fatty acid biosynthesis.
Sequence Caution Sequence=AAG40070.1; Type=Frameshift; Positions=150; Evidence={ECO:0000305};
Similarity Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily. {ECO:0000305}.
Subcellular Location Plastid, chloroplast {ECO:0000305}.
Subunit Homotetramer. {ECO:0000250}.
Tissue Specificity Expressed in flowers and siliques and at lower levels in roots and leaves (at protein level). {ECO:0000269|PubMed:10380806, ECO:0000269|PubMed:10715326}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010172 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18396215 RefSeq NP_565331 390 enoyl-[acyl-carrier-protein] reductase [NADH]

Identical Sequences to LMP010172 proteins

Reference Database Accession Length Protein Name
GI:18396215 GenBank AAM45010.1 390 putative enoyl-ACP reductase enr-A [Arabidopsis thaliana]
GI:18396215 GenBank ACH12874.1 390 Sequence 1828 from patent US 7396979
GI:18396215 GenBank AEC05988.1 390 enoyl-[acyl-carrier-protein] reductase [NADH] [Arabidopsis thaliana]
GI:18396215 GenBank AEC05989.1 390 enoyl-[acyl-carrier-protein] reductase [NADH] [Arabidopsis thaliana]
GI:18396215 RefSeq NP_849940.1 390 enoyl-[acyl-carrier-protein] reductase [NADH] [Arabidopsis thaliana]
GI:18396215 SwissProt Q9SLA8.1 390 RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic; Short=ENR; AltName: Full=NADH-dependent enoyl-ACP reductase 1; AltName: Full=Protein MOSAIC DEATH 1; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP010172 proteins

Reference Database Accession Length Protein Name
GI:18396215 EMBL CAA74175.1 390 enoyl-ACP reductase [Arabidopsis thaliana]
GI:18396215 EMBL CAC24636.1 390 unnamed protein product [Arabidopsis thaliana]
GI:18396215 GenBank AAF37208.1 390 enoyl-ACP reductase [Arabidopsis thaliana]
GI:18396215 GenBank ACH12873.1 390 Sequence 1827 from patent US 7396979
GI:18396215 GenBank ACH12875.1 390 Sequence 1829 from patent US 7396979
GI:18396215 GenBank AEL84957.1 390 Sequence 448 from patent US 7989676